Cultivar-specific seedling vigor and expression of a putative oxalate oxidase germin-like protein in sugar beet (Beta vulgaris L.)

Reyes, Benildo G. de los; McGrath, J. Mitchell

doi:10.1007/s00122-003-1229-9

Cited by 32 publications

(16 citation statements)

References 29 publications

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“…It appears that bicarbonate is produced by carbonic anhydrase in the perisperm and distrib-uted to the embryo. Oxalate oxidase behaves as a marker of germination vigor in sugarbeet (54). Note also that formate dehydrogenase (arrow 86 in Fig.…”

Section: Sugarbeet Seed Proteome Exhibits Tissue-specific Featuresmentioning

confidence: 92%

Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression

Catusse

Strub

Job

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

123

124

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Proteomic analysis of mature sugarbeet seeds led to the identification of 759 proteins and their specific tissue expression in root, cotyledons, and perisperm. In particular, the proteome of the perispermic storage tissue found in many seeds of the Caryophyllales is described here. The data allowed us to reconstruct in detail the metabolism of the seeds toward recapitulating facets of seed development and provided insights into complex behaviors such as germination. The seed appears to be well prepared to mobilize the major classes of reserves (the proteins, triglycerides, phytate, and starch) during germination, indicating that the preparation of the seed for germination is mainly achieved during its maturation on the mother plant. Furthermore, the data revealed several pathways that can contribute to seed vigor, an important agronomic trait defined as the potential to produce vigorous seedlings, such as glycine betaine accumulation in seeds. This study also identified several proteins that, to our knowledge, have not previously been described in seeds. For example, the data revealed that the sugarbeet seed can initiate translation either through the traditional cap-dependent mechanism or by a cap-independent process. The study of the tissue specificity of the seed proteome demonstrated a compartmentalization of metabolic activity between the roots, cotyledons, and perisperm, indicating a division of metabolic tasks between the various tissues. Furthermore, the perisperm, although it is known as a dead tissue, appears to be very active biochemically, playing multiple roles in distributing sugars and various metabolites to other tissues of the embryo.) is a dicotyledonous plant of the Amaranthaceae family that has a high economic importance because it is one of the two main sources of sucrose, the other being sugarcane. Furthermore, there is growing interest in the use of this crop to produce bioethanol. The quality of seed germination has a direct impact on the final yield of the culture and is conditioned by the number of plants issued from successful germinations and by the vigor of the seedlings, i.e., the potential to produce vigorous seedlings.The seed is the main form of dissemination of plants. It results from the conversion of a fertilized egg and contains a zygotic embryo (the future plant), one or more storage tissues [a triploid albumen, cotyledon(s), and perisperm] that accumulate the compounds necessary for the embryo's nutrition during germination, and seed coats to ensure the seed's protection against biotic and abiotic stress. A specific feature of sugarbeet is that the maternal nucellus is not fully digested during maturation and gives rise to the central perisperm, in which starch reserves accumulate (1-2). For most plant species growing in temperate climates, seed development ends with a phase of intense desiccation, then the embryo enters a dormant state, allowing its survival for many years. Two phytohormones, abscisic acid (ABA) and gibberellins (GA), play key roles in seed formation, dorm...

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Section: Sugarbeet Seed Proteome Exhibits Tissue-specific Featuresmentioning

confidence: 92%

Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression

Catusse

Strub

Job

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

123

124

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“…It is important to note that both ACH185 and USH20 were once dominant commercial hybrids grown in Michigan, and that emergence and stand establishment were not the primary reasons for their being supplanted by newer hybrids (the reasons were substantially related to disease pressure). These two hybrids were chosen because they differ markedly in their germination characteristics under stress, both in the field and in solution (McGrath et al 2000;de los Reyes and McGrath 2003). Control (unstressed) germination tests were performed by allowing 25 seeds (four replications per seedlot) to germinate on moistened pleated filter paper (TeKrony and Hardin 1966).…”

Section: Seed Germinationmentioning

confidence: 99%

“…2). Five gene products were monitored: aamylase (as a marker for carbohydrate catabolism in germinating seeds); acetyl-CoA acyltransferase (a b-oxidation enzyme that links lipid metabolism with the glyoxylate cycle); ICL and MS as markers for the glyoxylate pathway; and a germin-like protein as a sugar beet cultivar-specific marker for stress response and seedling vigor (de los Reyes and McGrath 2003). Additional transcripts coding for key enzymes of glycolysis (fructose bisphosphate aldolase, phosphoglucomutase, triose phosphate isomerase, and glyceraldehyde 3-phosphate dehydrogenase) were also monitored, but RNA gel blots showed that transcripts coding for these enzymes were highly abundant in both hybrids, with a slight induction in solution, and thus did not exhibit clear cultivar specificity at this level of analysis (data not shown).…”

Section: Gene Expression Profiles During Seedling Germinationmentioning

confidence: 99%

“…Differences in the efficiency of germination in solution between well emerging and poorly emerging cultivars are, however, reduced, and sometimes eliminated, when the germination medium is supplemented with H 2 O 2 (McGrath et al 2000). Cultivar differences in seedling vigor may be due in part to induced expression of a germin-like protein (a putative H 2 O 2 -producing oxalate oxidase), which, in high-vigor germplasm, could be physiologically equivalent to externally supplied H 2 O 2 (de los Reyes and McGrath 2003). Other mechanisms are also likely to influence seedling vigor.…”

Section: Introductionmentioning

confidence: 99%

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Differential induction of glyoxylate cycle enzymes by stress as a marker for seedling vigor in sugar beet (Beta vulgaris)

2003

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Significant differences in seedling vigor exist among sugar beet (Beta vulgaris) hybrids; however, traditional approaches to breeding enhanced vigor have not proven effective. Seedling vigor is a complex character, but presumably includes efficient mobilization of seed storage reserves during germination and efficient seedling growth in diverse environments. The involvement of lipid metabolism during germination of sugar beet under stress conditions was suggested by the isolation at high frequency of Expressed Sequence Tags (ESTs) with similarity to isocitrate lyase (EC 4.1.3.1). High-level expression of this glyoxylate cycle enzyme during germination and seedling emergence was also suggested by nucleotide sequencing of cDNA libraries obtained from a well emerging sugar beet hybrid during germination under stress. Genes involved in carbohydrate and lipid catabolism were differentially expressed in a strongly emerging hybrid, relative to a weakly emerging hybrid, during stress germination. Stress markedly reduced the levels of alpha-amylase transcripts in the weakly emerging hybrid. In contrast, the strongly emerging hybrid exhibited only a moderate reduction in alpha-amylase transcript levels under the same conditions, and showed large increases in the expression of genes involved in lipid metabolism, suggesting compensation by lipid for carbohydrate metabolism in the better emerging hybrid. Differential activity of the glyoxylate cycle thus appears to be a physiological marker that distinguishes between high- and low-vigor sugar beet cultivars. This finding suggests, for the first time, a biochemical target for selection for enhanced germination and improved emergence in sugar beet.

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“…The performance of a native herbivore of Nicotiana attenuata was found to be enhanced by silencing the germin-like gene (Lou and Baldwin, 2006). The recovery of GLPs from Beta vulgaris seedlings germinated under stress support their probable role during stress (De Los Reyes and McGrath, 2003).…”

Section: Introductionmentioning

confidence: 61%

Comparative analysis of regulatory elements in different germin-like protein gene promoters

Mahmood¹,

Nazar²,

Yasmin³

et al. 2010

Afr. J. Biotechnol.

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Germin and germin-like proteins (GLPs) the members of cupin superfamily of proteins, which are functionally most diverse proteins. Germin and GLPs have some unique features as they are highly resistant to proteases and to degradation by heat, high pH and detergents like Sodium dodecyl Sulphate (SDS). They are water soluble extracellular enzymatic protein that may also have Oxalate Oxidase (OxO), Superoxide dismutase (SOD) or ADP-glucose pyrophosphate or phosphodiestrase (AGPPase) activities. At the moment seven GLP gene promoter from different organisms have been studied and published. These all promoter sequences have been analyzed in this study. It was observed that these promoters have important regulatory elements, which are involved in various important functions. These elements have been compared on the basis of location, copy number, and distributed on positive and negative strands. It was also observed that some of these elements are common and remained conserved among all GLP promoters during evolution. Such regulatory elements are commonly observed in seed storage proteins, dehydration in response to light, senescence observed on exposure to dark and in elements specific for expression in pollen. Moreover, these common elements are reported to be expressed under environmental stresses (salt and pathogen attack) and to growth regulators.

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Cultivar-specific seedling vigor and expression of a putative oxalate oxidase germin-like protein in sugar beet (Beta vulgaris L.)

Cited by 32 publications

References 29 publications

Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression

Proteome-wide characterization of sugarbeet seed vigor and its tissue specific expression

Differential induction of glyoxylate cycle enzymes by stress as a marker for seedling vigor in sugar beet (Beta vulgaris)

Comparative analysis of regulatory elements in different germin-like protein gene promoters

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