CTX-M-Type Extended-Spectrum β-Lactamases Present in<i>Escherichia coli</i>from the Feces of Cattle in Ohio, United States

Wittum, Thomas E.; Mollenkopf, Dixie F.; Daniels, Joshua B.; Parkinson, Anne; Mathews, Jennifer; Fry, Pamela R.; Abley, Melanie; Gebreyes, Wondwossen A.

doi:10.1089/fpd.2010.0615

Cited by 74 publications

(70 citation statements)

References 21 publications

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“…The results highlighted that 52 isolates were positive for ESBL-producing. ESBL producing E. coli High contamination of raw-cow-milk was also reported by [19][20][21][22][23][24][25][26][27][28]. E. coli is accepted as a fecal contamination indicator in foods because of its presence in the intestinal tract.…”

Section: Discussionmentioning

confidence: 94%

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

Badri¹,

Ibrahim²,

Mohamed³

et al. 2017

Mol Biol

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Milk play a major role in human sources of nutrition and remain as the most important prominent in the Sudanese diet. Escherichia coli and Klebsiella pneumoniae are humans and animals opportunistic pathogens, responsible for a wide range of infections. The aim of this study was to evaluate the quality of the commercial available milk and to detect ESBL producing E. coli and K. pneumoniae from raw milk samples of cow in Al Jazirah state, Sudan. Seventy fresh row cow milk samples were collected and examined using standard microbiological methods, ESBL detection was performed on all the isolates by Ceftazidime screening test, those shows positive results by screening method were subjected to ESBL confirmatory test using Double-Disk Synergy Test and Molecular base detection using conventional PCR. Out of the 70 collected samples, 58 (82.8%) showed positive isolating result, the highest prevalence of the isolates was K. pneumoniae 36 (62%) followed by E. coli 22 (38%). The most resistance antibiotics against isolates was Ampicillin (98%), ESBL production was detected among 17 out of the 22 isolated E. coli (29.3%) and 26 (44.8%) out of the 36 isolated K. pneumoniae. The ESBL gene encoding the ESBL isolates was CTX-M gene representing 61% fellows by SHV gene (23%) and TEM gene (16%). ESBL-producing bacteria may also be transferred via waste milk to calves, thus further spreading antibiotic resistance in the farm environment.

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Section: Discussionmentioning

confidence: 94%

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

Badri¹,

Ibrahim²,

Mohamed³

et al. 2017

Mol Biol

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“…The ESBLs detected in food-producing animals or food in other non-European countries were: China (CTX-M-3, -13, -14, -15, -22, -24, -55, -64, -65; SHV-2, -12; TEM-52), Japan (CTX-M-2 and -14), and Senegal (SHV-12) (Ben Slama et al, 2010;Cardinale et al, 2001;Doi et al, 2009;Duan et al, 2006;Jouini et al, 2007;Kojima et al, 2005;Li et al, 2010a;Li et al, 2010b;Lim et al, 2009;Matsumoto et al, 2007;Rayamajhi et al, 2008;Shiraki et al, 2004;Tian et al, 2008;Wittum et al, 2010). These data show that mainly in Asian countries a higher diversity of CTX-Mvariants was observed.…”

Section: Esbl-subtypes Detected In E Coli and Salmonella From Food-pmentioning

confidence: 99%

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

2011

EFSA Journal

240

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The potential contribution of food-producing animals or foods to public health risks by ESBL and/or AmpCproducing bacteria is related to specific plasmid-mediated ESBL and/or AmpC genes encoded by a number of organisms. The predominant ESBL families encountered are CTX-M, TEM, and SHV; the predominant AmpCfamily is CMY. The most common genes associated with this resistance in animals are bla CTX-M-1 (the most commonly identified ESBL), and bla CTX-M-14 , followed by bla and bla Among the genes encoding AmpC-type β-lactamases, bla CMY-2 is the most common.The bacterial species most commonly identified with these genes are Escherichia coli and non-typhoidal Salmonella. ESBL/AmpC transmission is mainly driven by integrons, insertion sequences, transposons and plasmids, some of which are homologous in isolates from both food-production animals and humans. Cefotaxime is used as the drug of choice for optimum detection of bla ESBL and/or bla AmpC genes. The preferred method for isolation of ESBL-and/or AmpC-producers is screening on selective agar preceded by selective enrichment in a broth.The establishment of risk factors for occurrence of ESBL/AmpC-producing bacteria is particularly complicated by the data unavailability or lack of its accuracy. The use of antimicrobials is a risk factor for the selection and spread of resistant clones, resistance genes and plasmids. Since most ESBL-and AmpC-producing strains carry additional resistances to other commonly-used veterinary drugs, generic antimicrobial use is a risk factor for ESBL/AmpC and it is not restricted specifically to the use of cephalosporins. An additional risk factor is extensive trade of animals in EU MS. There are no data on the comparative efficiency of individual control options in reducing public health risks caused by ESBL and/or AmpC-producing bacteria related to food-producing animals. Prioritisation is complex, but it is considered that a highly effective control option would be to stop all uses of cephalosporins/systemically active 3 rd /4 th generation cephalosporins, or to restrict their use (use only allowed under specific circumstances). As co-resistance is an important issue, it is also of high priority to decrease the total antimicrobial use in animal production in the EU. KEY WORDSResistance, ESBLs, AmpC, occurrence, transmission, control options, public health microbiology. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum beta (β)-lactamases (ESBL) and/or AmpC β-lactamases (AmpC) in food and foodproducing animals. In particular, the Panel was asked: (i) to propose a definition of the ESBL-and/or AmpC-producing bacterial strains and genes relevant for public health and linked to food-producing animals or food borne transmission; (ii) to review the information on the epidemiology of acquired resistance to broad spectrum cephalosporins including the genes coding for ...

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“…With the current global target of "one world, one health", efforts are being intensified to detect sources of antibacterial resistance (especially β-lactam resistance) in order to curb its spread in both humans and animals (Wall, 2014). Surveillance studies to assess foodproducing animals as potential reservoirs and disseminators of ESBL-resistant bacteria have been conducted in several countries in Europe (Aestrup et al, 2006;Meunier et al, 2006;Cloeckaert et al, 2007;Girlich et al, 2007;Smet et al, 2008;Wu et al, 2008;Cortes et al, 2010;Goncalves et al, 2010;Geser et al, 2011;Geser et al 2012;Hammerrum et al, 2014), Asia (Duan et al, 2006;Tian et al, 2009;Yuan et al, 2009;Li et al, 2014;Wang et al, 2014), Africa (Jouini et al, 2007;Ben Sallem et al, 2012), North America (Wittum et al, 2010) and South America (Fernandes et al, 2009). Unfortunately, there is paucity of information on ESBLresistant bacteria in food-producing animals in Nigeria.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

et al. 2015

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This study was conducted to isolate generic extended-spectrum β-lactam (ESBL)-resistant enterobacteria from pigs reared in Enugu State Southeast, Nigeria and determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 190, randomly selected, apparently healthy pigs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 2 µg/ml of cefotaxime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 46 ESBL-resistant enterobacterial isolates, 4 (8.7%) were Escherichia coli, 11 (23.9%) were Salmonella species, while 31 (67.4%) were Klebsiella species. Resistance of the Salmonella isolates was 45.5% to ciprofloxacin, 36.4% to ofloxacin and levofloxacin, 9.1% to norfloxacin, amikacin and gentamicin, 27.3% to streptomycin, 72.7% to chloramphenicol and 90.9% to tetracycline. Resistance of the Klebsiella isolates was 93.5% to ampicillin, 12.9% to ciprofloxacin, 19.4% to ofloxacin and levofloxacin, 9.7% to norfloxacin and streptomycin, 64.5% to chloramphenicol and 38.7% to tetracycline. Resistance of the E. coli isolates was 100% to gentamicin, 75% to ampicillin and streptomycin, 50% to ciprofloxacin, norfloxacin, chloramphenicol and tetracycline, and 25% to ofloxacin, levofloxacin and amikacin. All the isolates were resistant to ceftriaxone, cefotaxime, ceftazidime, cefepime, cefpodoxime, amoxicillin/clavulanic acid and aztreonam. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 54.8% for Klebsiella, 90.9% for Salmonella and 100% for E. coli, respectively. This study has shown that pigs reared in Enugu State Southeast, Nigeria, are colonized by ESBL-resistant Enterobactericeae and are potential reservoirs and disseminators of these organisms.

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CTX-M-Type Extended-Spectrum β-Lactamases Present inEscherichia colifrom the Feces of Cattle in Ohio, United States

Cited by 74 publications

References 21 publications

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumoniae Isolated from Raw Milk Samples in Al Jazirah State, Sudan

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

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