Crystalline Vitamin B
            <sub>12</sub>

Rickes, Edward L.; Brink, Norman G.; Koniuszy, Frank R.; Wood, Thomas H.; Folkers, Karl

doi:10.1126/science.107.2781.396

Cited by 510 publications

(122 citation statements)

References 5 publications

Supporting

Mentioning

118

Contrasting

Unclassified

Order By: Relevance

“…Three years after the discovery of vitamin B 12 (62,64), pseudovitamin B 12 was isolated from an incompletely identified microorganism that was obtained from rumen contents (20). In 1958 the coenzyme form of pseudovitamin B 12 (i.e., pseudocoenzyme B 12 ) was discovered and identified by UV and visible (UV-Vis) spectroscopy to be the native cofactor of Clostridium tetanomorphum (2).…”

mentioning

confidence: 99%

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B₁₂and Factor A

et al. 2000

View full text Add to dashboard Cite

The corrinoids from the obligate anaerobe Clostridium cochlearium were extracted as a mixture of Co ␤ -cyano derivatives. From 50 g of frozen cells, approximately 2 mg (1.5 mol) of B 12 derivatives was obtained as a crystalline sample. Analysis of the corrinoid sample of C. cochlearium by a combination of high-pressure liquid chromatography and UV-Vis absorbance spectroscopy revealed the presence of three cyano corrinoids in a ratio of about 3:1:1. The spectroscopic data acquired for the sample indicated the main components to be pseudovitamin B 12 (Co ␤ -cyano-7؆-adeninylcobamide) (60%) and factor A (Co ␤ -cyano-7؆-[2-methyl]adeninylcobamide) (20%). Authentic pseudovitamin B 12 was prepared by guided biosynthesis from cobinamide and adenine. Both pseudovitamin B 12 and its homologue, factor A, were subjected to complete spectroscopic analysis by UV-Vis, circular dichroism, mass spectrometry, and by one-and two-dimensional 1 H, 13 C-, and 15 N nuclear magnetic resonance (NMR) spectroscopy. The third component was indicated by the mass spectra to be an isomer of factor A and is likely (according to NMR) to be 7؆-[N 6 -methyl]-adeninylcobamide, a previously unknown corrinoid. C. cochlearium thus biosynthesizes as its native "complete" B 12 cofactors the 7؆-adeninylcobamides and two homologous corrinoids, in which the nucleotide base is a methylated adenine.

show abstract

mentioning

confidence: 99%

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B₁₂and Factor A

et al. 2000

View full text Add to dashboard Cite

show abstract

“…Az antianaemiás hatóanyagot májból és veséből Smith, illetve Rickes és mtsai 1948-ban izolálták [14,15]. A ké-miailag egységes és B 12 -vitamin elnevezést elnyert vegyület szerkezetét Dorothy Hodgkin és mtsai 1956-ban röntgenkrisztallográfi ával igazolták.…”

Section: A Hatóanyag Gyógyszerré Fejlesztéseunclassified

History of the therapy of pernicious anemia

Jeney

2013

Orvosi Hetilap

View full text Add to dashboard Cite

A kivéreztetett kísérleti állatok vérregenerációját Whipple a nyers máj, Jeney és Jobling pedig a máj vizes kivonatának adagolásával tudta fokozni. A májetetés terápiás hatékonyságát Minot és Murphy 45, anaemia perniciosás betegen 1926-ban igazolták. Whipple, Minot és Murphy a májterápia sikeres alkalmazásáért 1934-ben Nobel-díjban része-sült. A B 12 -vitaminnak elnevezett hatóanyagot 1948-ban sikerült májból kivonni. Lucy Wills ugyanezen években alultáplált, terhes asszonyok anaemiáját kedvezően tudta befolyásolni élesztőkivonattal, ami a folsav felfedezéséhez vezetett. Mindkét gyógyszer hatásmódjának tanulmányozása jelentősen gazdagította patofi ziológiai ismereteinket, továbbá újabb terápiás alkalmazásokat tárt fel. Orv. Hetil., 2013, 154, 1754-1758 Kulcsszavak: anaemia perniciosa, májterápia, B 12 -vitamin, folsav History of the therapy of pernicious anemiaIncreased blood cell regeneration in exsanguinated experimental animals treated either with liver or with aqueous liver extracts was reported by Whipple and by Jeney and Jobling, respectively. These fi ndings stimulated Minot and Murphy to provide evidence for the effi cacy of liver against anaemia in clinical studies. After oral administration of liver (45-50 g per day) for 45 patients with anaemia perniciosa improvement of the hematological status was demonstrated. Consequently, for proving the therapeutic value of liver therapy Whipple, Minot and Murphy received Nobel price in 1934. The isolation of the antianemic factor from the liver has been succeded in 1948 and designated as vitamin B12. At the same time Lucy Wills applied yeast for the treatment of pregnant women with anemia related to undernourisment. The conclusions of this study inspired the discovery of folate. The detailed investigation of the mode of action of vitamin B12 and folate enriched our knowledge in the area of pathophysiology and extended the clinical application of these two drugs. A betegek életmódjának megfi gyelése nyomán Osler az anaemia perniciosa kezelésére a Fowler által bevezetett arzéncseppeket ajánlotta, ugyanakkor tankönyvében beszámolt az állati táplálék (hús, máj) fogyasztásának jótékony hatásáról és az alultápláltság kóroki szerepéről [4].

show abstract

“…It took another 22 years for a pure antianaemic factor to be isolated, although, admittedly, the Second World War intervened; in 1948, an American group led by Karl Folkers and an English group led by E. Lester-Smith published, within weeks of each other, the isolation of a red crystalline substance termed vitamin B 12 and subsequently renamed cobalamin (Lester-Smith, l948; Rickes et al, 1948). The slow progress was because the fractions that were isolated from liver had to be tested for activity in untreated PA patients, although this step was bypassed in the final stages using a microbiological assay with Lactobacillus lactis for the antianaemic factor by the American group (Shorb, 1947).…”

Section: Isolation Of the Antianaemic Factormentioning

confidence: 99%

A history of pernicious anaemia

Chanarin¹

2000

Br J Haematol

View full text Add to dashboard Cite

Crystalline Vitamin B ₁₂

Cited by 510 publications

References 5 publications

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B₁₂and Factor A

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B₁₂and Factor A

History of the therapy of pernicious anemia

A history of pernicious anaemia

Contact Info

Product

Resources

About

Crystalline Vitamin B 12

Cited by 510 publications

References 5 publications

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B12and Factor A

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B12and Factor A

History of the therapy of pernicious anemia

A history of pernicious anaemia

Contact Info

Product

Resources

About

Crystalline Vitamin B ₁₂

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B₁₂and Factor A

Native Corrinoids fromClostridium cochleariumAre Adeninylcobamides: Spectroscopic Analysis and Identification of Pseudovitamin B₁₂and Factor A