Crystal structures of the isochorismatase domains from Vibrio anguillarum

Chorismate- and isochorismate converting enzymes: versatile catalysts acting on an important metabolic node

“…Isochorismatases have the characteristic a/b-fold of the isochorismatase hydrolase superfamily but lack the catalytic triad that is common to most members of this superfamily. 44,98,99,101 The structure is characterised by a central six-stranded b-sheet that is covered on one side by three a-helices and a loop; this also defines the active site. On the opposite side, usually one large a-helix is spanned over the central sheet (Fig.…”

“…3F). 44,98,99,101 In the active site, isochorismatases feature a hydrophobic pocket stabilising the non-substituted side of the cyclohexadiene ring (Ile, Val, Leu, 2Â Phe, 2Â Tyr, Trp). A positively charged (Arg) and a polar (Gln) residue coordinate the carboxyl group at C1 while the enolpyruvyl carboxylate is held in place by backbone interactions (Fig.…”

Iron Acquisition Strategies of Vibrio anguillarum

“…Most are located on the plasmid pJM1 or pJM1-like plasmids while some are on the chromosomes (Figure 1). First, chorismate is catalyzed stepwise by a series of proteins, AngC/VabC (isochrorismate synthase), AngB/VabB (isochrorismatase; Du et al, 2017), and VabA (2,3-dihydro-2,3-dehydroxybenzoate dehydrogenase), to synthesize DHBA (Figure 2; Alice et al, 2005; Balado et al, 2008). In later steps, phosphopantetheine transferase AngD is required to transfer a phosphopantetheinyl moiety to a serine residue of AngB and AngM (Balado et al, 2008).…”