Iron Acquisition Strategies of Vibrio anguillarum

Li, Yingjie; Ma, Qingjun

doi:10.3389/fcimb.2017.00342

Cited by 34 publications

(25 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…anguillarum 775 carries both chromosomal (Chr I) and plasmid-born (pJM1) genes for anguibactin biosynthesis, most of them on the plasmid (i.e., angB, angD, angCE, angN, angR, angM, angH, angT, angU) [ 40 , 65 , 66 ]. The plasmid-carried genes have been hypothesized to spread e.g., into Vibrio harveyi , or vice versa (see [ 67 ]). Similarly, a piscibactin biosynthesis cluster is located on a conjugative plasmid (pPHDP70) of a highly virulent P .…”

Section: Resultsmentioning

confidence: 99%

Distribution of siderophore gene systems on a Vibrionaceae phylogeny: Database searches, phylogenetic analyses and evolutionary perspectives

et al. 2018

View full text Add to dashboard Cite

Siderophores are small molecules synthesized and secreted by bacteria and fungi to scavenge iron. Extracellular ferri-siderohores are recognized by cognate receptors on the cell surface for transport over membranes. Several siderophore systems from Vibrionaceae representatives are known and well understood, e.g., the molecular structure of the siderophore, the biosynthesis gene cluster and pathway, and the gene expression pattern. Less is known about how these systems are distributed among the ~140 Vibrionaceae species, and which evolutionary processes contributed to the present-day distribution. In this work, we compiled existing knowledge on siderophore biosynthesis systems and siderophore receptors from Vibrionaceae and used phylogenetic analyses to investigate their organization, distribution, origin and evolution. Through literature searches, we identified nine different siderophore biosynthesis systems and thirteen siderophore receptors in Vibrionaceae. Homologs were identified by BLAST searches, and the results were mapped onto a Vibrionaceae phylogeny. We identified 81 biosynthetic systems distributed in 45 Vibrionaceae species and 16 unclassified Vibrionaceae strains, and 409 receptors in 89 Vibrionaceae species and 49 unclassified Vibrionaceae strains. The majority of taxa are associated with at least one type of siderophore biosynthesis system, some (e.g., aerobactin and vibrioferrin) of which are widely distributed in the family, whereas others (i.e., bisucaberin and vibriobactin) are found in one lineage. Cognate receptors are found more widespread. Phylogenetic analysis of three siderophore systems (piscibactin, vibrioferrin and aerobactin) show that their present-day distribution can be explained by an old insertion into Vibrionaceae, followed mainly by stable vertical evolution and extensive loss, and some cases of horizontal gene transfers. The present work provides an up to date overview of the distribution of siderophore-based iron acquisition systems in Vibrionaceae, and presents phylogenetic analysis of these systems. Our results suggest that the present-day distribution is a result of several evolutionary processes, such as old and new gene acquisitions, gene loss, and both vertical and horizontal gene transfers.

show abstract

Section: Resultsmentioning

confidence: 99%

Distribution of siderophore gene systems on a Vibrionaceae phylogeny: Database searches, phylogenetic analyses and evolutionary perspectives

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It is well known that bacterial virulence is a multifactorial trait and that pathogenic bacteria must have a number of factors that enable them to colonize the host and establish an infection ( Miller et al, 1989 ). The virulence-related factors identified in V. anguillarum so far include those related to chemotaxis and motility ( O’Toole et al, 1996 ; Ormonde et al, 2000 ), adhesion ( Wang et al, 1998 ), invasion ( Croxatto et al, 2007 ), secretion of extracellular enzymes ( Denkin and Nelson, 2004 ; Rodkhum et al, 2005 ; Li et al, 2008 ) and several iron uptake mechanisms ( Li and Ma, 2017 ). Despite this knowledge, the pathogenesis of V. anguillarum is not completely understood ( Hickey and Lee, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…Iron restriction is an important host defense strategy, thus successful pathogens must possess mechanisms to acquire iron from host sources in order to cause disease. In V. anguillarum two catechol siderophores, vanchrobactin and anguibactin, have been described ( Actis et al, 1986 ; Soengas et al, 2006 ; Lemos et al, 2010 ; Li and Ma, 2017 ). While anguibactin is encoded by pJM1-type plasmids and is restricted to some virulent strains of serotype O1, vanchrobactin is a chromosomally encoded siderophore that is widespread in all V. anguillarum isolates either environmental or pathogenic ( Lemos et al, 2010 ; Li and Ma, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

The Siderophore Piscibactin Is a Relevant Virulence Factor for Vibrio anguillarum Favored at Low Temperatures

et al. 2018

View full text Add to dashboard Cite

Vibrio anguillarum causes vibriosis, a hemorrhagic septicaemia that affects many cultured marine fish species worldwide. Two catechol siderophores, vanchrobactin and anguibactin, were previously identified in this bacterium. While vanchrobactin is a chromosomally encoded system widespread in all pathogenic and environmental strains, anguibactin is a plasmid-encoded system restricted to serotype O1 strains. In this work, we have characterized, from a serotype O2 strain producing vanchrobactin, a novel genomic island containing a cluster of genes that would encode the synthesis of piscibactin, a siderophore firstly described in the fish pathogen Photobacterium damselae subsp. piscicida. The chemical characterization of this siderophore confirmed that some strains of V. anguillarum produce piscibactin. An in silico analysis of the available genomes showed that this genomic island is present in many of the highly pathogenic V. anguillarum strains lacking the anguibactin system. The construction of single and double biosynthetic mutants for vanchrobactin and piscibactin allowed us to study the contribution of each siderophore to iron uptake, cell fitness, and virulence. Although both siderophores are simultaneously produced, piscibactin constitute a key virulence factor to infect fish, while vanchrobactin seems to have a secondary role in virulence. In addition, a transcriptional analysis of the gene cluster encoding piscibactin in V. anguillarum showed that synthesis of this siderophore is favored at low temperatures, being the transcriptional activity of the biosynthetic genes three-times higher at 18°C than at 25°C. We also show that iron levels and temperature contribute to balance the synthesis of both siderophores.

show abstract

“…In sea bass, V. anguillarum evades the innate immune response by inhibiting the respiratory burst reaction in macrophages and by downregulating the expression of apoptotic caspases (7). In addition, many Vibrio strains have siderophore-dependent and/or siderophore-independent ironacquisition mechanisms [reviewed in (8,9)], which enables them to use iron for growth even if iron-binding, fish proteins such as ferritin and transferrin are present.…”

Section: Introductionmentioning

confidence: 99%

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

et al. 2020

View full text Add to dashboard Cite

Vibrio anguillarum causes high mortality in European sea bass (Dicentrarchus labrax) larviculture and is a hindering factor for successful sustainable aquaculture of this commercially valuable species. Priming of the innate immune system through administration of immunostimulants has become an important approach to control disease outbreaks in marine fish larviculture. This study was conducted to evaluate immunostimulation by Escherichia coli HSP70 (DnaK) in axenic European sea bass larvae in order to protect the larvae against vibriosis. DnaK stimulates the immune response in crustaceans and juvenile fish against bacterial infections. The use of axenic fish larvae allows to study immunostimulation in the absence of an interfering microbial community. At 7 days post-hatching, larvae received a single dose of alginate encapsulated recombinant DnaK. Two non-treated control groups in which animals either received empty alginate microparticles (C1) or no alginante microparticles (C2 and C3) were included in the study. Eighteen hours later, all larvae, except the ones from group C3 (non-infected control) were challenged with V. anguillarum (10 5 CFU, bath infection). Mortality was daily recorded until 120 h post infection and at 18, 24, and 36 h post infection, larvae were sampled for expression of immune related genes. Results showed that V. anguillarum induced an immune response in axenic sea bass larvae but that the innate immune response was incapable to protect the larvae against deadly septicaemic disease. In addition, we showed that administration of alginate encapsulated DnaK to axenic European sea bass larvae at DAH7 resulted in a significant, DnaK dose dependent, upreglation of immune sensor, regulatory and effector genes. Significant upregulation of cxcr4, cas1 and especially of hep and dic was correlated with significant higher survival rates in V. anguillarum infected larvae. In the future recombinant DnaK might perhaps be used as a novel immunostimulant in sea bass larviculture.

show abstract

Iron Acquisition Strategies of Vibrio anguillarum

Cited by 34 publications

References 102 publications

Distribution of siderophore gene systems on a Vibrionaceae phylogeny: Database searches, phylogenetic analyses and evolutionary perspectives

Distribution of siderophore gene systems on a Vibrionaceae phylogeny: Database searches, phylogenetic analyses and evolutionary perspectives

The Siderophore Piscibactin Is a Relevant Virulence Factor for Vibrio anguillarum Favored at Low Temperatures

Recombinant DnaK Orally Administered Protects Axenic European Sea Bass Against Vibriosis

Contact Info

Product

Resources

About