Complete genome sequence of the marine fish pathogen Vibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential for in vivo infection

Yang, Guanhua; Wang, Cheng; Wang, Xuetong; Ma, Ruiqing; Zheng, Huajun; Liu, Qin; Zhang, Yuanxing; Ma, Yue; Wang, Qiyao

doi:10.1016/j.micres.2018.08.011

Cited by 11 publications

(18 citation statements)

References 78 publications

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“…While major porines OmpA and OmpV were 3.5- and 6.4-fold induced under iron starvation at 25°C, transcription of ompC increased only at low temperature (11.5-fold). Vibrio anguillarum genome harbors two T6SS (Guanhua et al, 2018). T6SS1 and T6SS2 were induced 3- and 10-fold, respectively, under iron starvation.…”

Section: Resultsmentioning

confidence: 99%

“…We found a significant up-regulation of genes involved in LPS biosynthesis, assembly, and export under iron starvation conditions, but additionally, their expression increased at low temperature (Supplementary Table S4). Chemotaxis and motility have been recognized as virulence-related factors (Larsen, 2004; Guanhua et al, 2018). Although V. anguillarum RV22 is motile under the three conditions tested (Supplementary Figure S2), our results showed that chemotaxis and flagellum-related genes were significantly down-regulated under both low iron availability and low temperature.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, our results show that while T6SS1 is preferentially expressed at 25°C, T6SS2 shows the highest expression at 15°C. Although no significant loss of virulence was observed in V. anguillarum when T6SS2 was inactivated (Weber et al, 2009; Tang et al, 2016), recent works showed that inactivation of either T6SS1 or T6SS2 decreased the fitness of the bacterium within the fish (Guanhua et al, 2018). It was also reported that T6SS2 would regulate the expression of extracellular proteases via the stress-response regulator RpoS and the quorum sensing regulator VanT (Weber et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…It was postulated that piscibactin production would enhance niche flexibility conferring wide virulence properties even at low temperatures (Balado et al, 2018). In addition, some recent studies have demonstrated that environmental stress could affect the expression of virulence genes that are involved in the ability of V. anguillarum to cause infection (Crisafi et al, 2014; Guanhua et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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The Expression of Virulence Factors in Vibrio anguillarum Is Dually Regulated by Iron Levels and Temperature

2019

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Vibrio anguillarum causes a hemorrhagic septicemia that affects cold- and warm-water adapted fish species. The main goal of this work was to determine the temperature-dependent changes in the virulence factors that could explain the virulence properties of V. anguillarum for fish cultivated at different temperatures. We have found that although the optimal growth temperature is around 25°C, the degree of virulence of V. anguillarum RV22 is higher at 15°C. To explain this result, an RNA-Seq analysis was performed to compare the whole transcriptome profile of V. anguillarum RV22 cultured under low-iron availability at either 25 or 15°C, which would mimic the conditions that V. anguillarum finds during colonization of fish cultivated at warm- or cold-water temperatures. The comparative analysis of transcriptomes at high- and low-iron conditions showed profound metabolic adaptations to grow under low iron. These changes were characterized by a down-regulation of the energetic metabolism and the induction of virulence-related factors like biosynthesis of LPS, production of hemolysins and lysozyme, membrane transport, heme uptake, or production of siderophores. However, the expression pattern of virulence factors under iron limitation showed interesting differences at warm and cold temperatures. Chemotaxis, motility, as well as the T6SS1 genes are expressed at higher levels at 25°C than at 15°C. By contrast, hemolysin RTX pore-forming toxin, T6SS2, and the genes associated with exopolysaccharides synthesis were preferentially expressed at 15°C. Notably, at this temperature, the siderophore piscibactin system was strongly up-regulated. In contrast, at 25°C, piscibactin genes were down-regulated and the vanchrobactin siderophore system seems to supply all the necessary iron to the cell. The results showed that V. anguillarum adjusts the expression of virulence factors responding to two environmental signals, iron levels and temperature. Thus, the relative relevance of each virulence factor for each fish species could vary depending on the water temperature. The results give clues about the physiological adaptations that allow V. anguillarum to cause infections in different fishes and could be relevant for vaccine development against fish vibriosis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Expression of Virulence Factors in Vibrio anguillarum Is Dually Regulated by Iron Levels and Temperature

2019

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“…The predisposing factors inducing vibriosis include bad water quality, pollution, high temperatures, osmotic changes, periods of fish nutrient debrivation, inadequate diet composition, and high population density [2]. These environmental stressors could affect the expression of virulence genes that are involved in the pathogenicity of V. anguillarum [9]. Saprolegniasis is responsible for significant economic losses in hatcheries and freshwater fish farms across the globe.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Pathogenic Vibrio anguillarumAmong Oreochromis niloticusFish Fingerlings Infected with Saprolegniasis Around Qarun Lake

et al. 2021

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V IBRIO anguillarum causes severe economic losses in fish and shellfish species and poses public health concerns. This study aimed to provide some information about the prevalence and virulence determinants of V. anguillarum isolated from the farmed Oreochromisniloticus fish fingerlings nearby Qarun Lake, El-Fayoum Governorate, Egypt. PCRtargeting the rpoA (RNA polymerase alpha subunit)gene was done forconfirmation of Vibriospp. Moreover, V. anguillarum isolates were biochemically identified and confirmed by 16S rRNA gene sequence.V. anguillarum isolates produced caseinase, gelatinase, lipase, lecithinase, haemolysins, and possessed biofilm formation. The virulence-related genes;empA, vah1, vah3 and vah4, flaA,angM, and angRwere detected in all tested isolates. Therefore, proper identification of V.anguillarumis crucial to better understand the ecology and distribution patterns of such pathogen within Egyptian aquaculture. Moreover, the water quality in these farms near Qarun Lake was less supportive for larval rearing activities of O. niloticus fish.

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Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

Song,

Soomro,

Dong

et al. 2023

J. Ocean. Limnol.

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Complete genome sequence of the marine fish pathogen Vibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential for in vivo infection

Cited by 11 publications

References 78 publications

The Expression of Virulence Factors in Vibrio anguillarum Is Dually Regulated by Iron Levels and Temperature

The Expression of Virulence Factors in Vibrio anguillarum Is Dually Regulated by Iron Levels and Temperature

Prevalence of Pathogenic Vibrio anguillarumAmong Oreochromis niloticusFish Fingerlings Infected with Saprolegniasis Around Qarun Lake

Transcriptome analysis reveals immune-related genes in tissues of Vibrio anguillarum-infected turbot Scophthalmus maximus

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