Cryopreserved skin epithelial cell sheet combined with acellular amniotic membrane as an off-the-shelf scaffold for urethral regeneration

Zhang, Dongliang; Gu, Yubo; Shao, Jianhua; Zhou, Shengyan; Hou, Junyi; Song, Lujie; Wang, Xiang

doi:10.1016/j.msec.2021.111926

Cited by 15 publications

(12 citation statements)

References 26 publications

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“…Cell metabolism can be affected by cytotoxic materials. After the Calcein AM-PI staining, the living cells were green and the dead cells were red ( Zhang et al, 2021 ). From the staining results of live/dead cells cultured to 3 days ( Figure 5 ), the cells cultured by FDBM extraction solution grow well compared with the control group, and there is no significant difference in the proportion of dead cells, indicating that the FDBM scaffold has no cytotoxicity.…”

Section: Resultsmentioning

confidence: 99%

Preparation of fish decalcified bone matrix and its bone repair effect in rats

Liu

Jiang²,

Wang³

et al. 2023

Front. Bioeng. Biotechnol.

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Decalcified bone matrix has great potential and application prospects in the repair of bone defects due to its good biocompatibility and osteogenic activity. In order to verify whether fish decalcified bone matrix (FDBM) has similar structure and efficacy, this study used the principle of HCl decalcification to prepare the FDBM by using fresh halibut bone as the raw material, and then degreasing, decalcifying, dehydrating and freeze-drying it. Its physicochemical properties were analyzed by scanning electron microscopy and other methods, and then its biocompatibility was tested by in vitro and in vivo experiments. At the same time, an animal model of femoral defect in rats was established, and commercially available bovine decalcified bone matrix (BDBM) was used as the control group, and the area of femoral defect in rats was filled with the two materials respectively. The changes in the implant material and the repair of the defect area were observed by various aspects such as imaging and histology, and its osteoinductive repair capacity and degradation properties were studied. The experiments showed that the FDBM is a form of biomaterial with high bone repair capacity and lower economic cost than other related materials such as bovine decalcified bone matrix. FDBM is simpler to extract and the raw materials are more abundant, which can greatly improve the utilization of marine resources. Our results show that FDBM not only has a good repair effect on bone defects, but also has good physicochemical properties, biosafety and cell adhesion, and is a promising medical biomaterial for the treatment of bone defects, which can basically meet the clinical requirements for bone tissue repair engineering materials.

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Section: Resultsmentioning

confidence: 99%

Preparation of fish decalcified bone matrix and its bone repair effect in rats

Liu

Jiang²,

Wang³

et al. 2023

Front. Bioeng. Biotechnol.

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“…Cytotoxic materials can affect cell metabolism. After the Calcein AM-PI staining, the living cells were green and the dead cells were red ( Zhang et al, 2021 ). Figure 2A shows that both the control group and the TADM group have obvious proliferation trend within three and 5 days.…”

Section: Resultsmentioning

confidence: 99%

Application of Tilapia Skin Acellular Dermal Matrix to Induce Acute Skin Wound Repair in Rats

Wang

et al. 2022

Front. Bioeng. Biotechnol.

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Extracellular matrix (ECM) material with good biological activity is essential to simulate cell growth microenvironment, induce cell infiltration and angiogenesis, and promote the repair of large area acute skin wound. In this study, tilapia skin acellular dermal matrix (TADM) was prepared to simulate ECM microenvironment, which can promote substantial area acute wound healing in rats. The main component of TADM is type I collagen, which has good physical and chemical properties, biological activity and cell adhesion. TADM is a form of biomaterial with low immunogenicity, low risk of prion infection and lower economic cost than other related materials such as mammalian collagen biomaterials. Our results show that TADM can guide cell infiltration, angiogenesis, regulate the expression and secretion of inflammatory and skin repair correlated factors to promote tissue healing.

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“…Zhang et al [ 92 ] combined rabbit skin epithelial cell sheets (SECs) with decellularized AMs for freezing treatment to reconstruct the rabbit urethra. The study found that the combination with decellularized AMs can reduce the cryopreservation damage of SECs.…”

Section: Scaffolded Graftsmentioning

confidence: 99%

Tailor-made natural and synthetic grafts for precise urethral reconstruction

Tan

Tang

et al. 2022

J Nanobiotechnol

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Injuries to the urethra can be caused by malformations, trauma, inflammation, or carcinoma, and reconstruction of the injured urethra is still a significant challenge in clinical urology. Implanting grafts for urethroplasty and end-to-end anastomosis are typical clinical interventions for urethral injury. However, complications and high recurrence rates remain unsatisfactory. To address this, urethral tissue engineering provides a promising modality for urethral repair. Additionally, developing tailor-made biomimetic natural and synthetic grafts is of great significance for urethral reconstruction. In this work, tailor-made biomimetic natural and synthetic grafts are divided into scaffold-free and scaffolded grafts according to their structures, and the influence of different graft structures on urethral reconstruction is discussed. In addition, future development and potential clinical application strategies of future urethral reconstruction grafts are predicted.

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Cryopreserved skin epithelial cell sheet combined with acellular amniotic membrane as an off-the-shelf scaffold for urethral regeneration

Cited by 15 publications

References 26 publications

Preparation of fish decalcified bone matrix and its bone repair effect in rats

Preparation of fish decalcified bone matrix and its bone repair effect in rats

Application of Tilapia Skin Acellular Dermal Matrix to Induce Acute Skin Wound Repair in Rats

Tailor-made natural and synthetic grafts for precise urethral reconstruction

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