Tailor-made natural and synthetic grafts for precise urethral reconstruction

Tan, Qinyuan; Le, Hanxiang; Tang, Chao; Zhang, Ming; Yang, Weihua; Hong, Yazhao; Wang, Xiaoqing

doi:10.1186/s12951-022-01599-z

Cited by 8 publications

(9 citation statements)

References 130 publications

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“…The cell sheet technique may be a promising novel approach in the field of urethral regeneration. A cell sheet retains the extracellular matrix with active factors, which facilitates local cell proliferation and regeneration [ 98 ]. It does not require enzymatic digestion and has a higher cell viability rate.…”

Section: Cell Sheet Technologymentioning

confidence: 99%

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Cell-Based Therapy for Urethral Regeneration: A Narrative Review and Future Perspectives

Jin,

Zhao,

Yang

et al. 2023

Biomedicines

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Urethral stricture is a common urological disease that seriously affects quality of life. Urethroplasty with grafts is the primary treatment, but the autografts used in clinical practice have unavoidable disadvantages, which have contributed to the development of urethral tissue engineering. Using various types of seed cells in combination with biomaterials to construct a tissue-engineered urethra provides a new treatment method to repair long-segment urethral strictures. To date, various cell types have been explored and applied in the field of urethral regeneration. However, no optimal strategy for the source, selection, and application conditions of the cells is available. This review systematically summarizes the use of various cell types in urethral regeneration and their characteristics in recent years and discusses possible future directions of cell-based therapies.

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Section: Cell Sheet Technologymentioning

confidence: 99%

“…The advantage of cell sheet technology is that it removes the influence of scaffold material degradation [ 98 ]. Notably, however, the current cost required to produce patient-derived cell sheets limits their widespread use.…”

Section: Cell Sheet Technologymentioning

confidence: 99%

Cell-Based Therapy for Urethral Regeneration: A Narrative Review and Future Perspectives

Jin,

Zhao,

Yang

et al. 2023

Biomedicines

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“…Remarkably, after six years of implantation, all patients exhibited functional urethras, indicating that synthetic polymers such as PLGA can be successfully assimilated by the human body [ 50 ]. It is also possible to combine different types of synthetic materials in order to form a scaffold [ 51 , 52 , 53 ].…”

Section: Principles Of Utementioning

confidence: 99%

Biological Macromolecule-Based Scaffolds for Urethra Reconstruction

et al. 2023

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Urethral reconstruction strategies are limited with many associated drawbacks. In this context, the main challenge is the unavailability of a suitable tissue that can endure urine exposure. However, most of the used tissues in clinical practices are non-specialized grafts that finally fail to prevent urine leakage. Tissue engineering has offered novel solutions to address this dilemma. In this technology, scaffolding biomaterials characteristics are of prime importance. Biological macromolecules are naturally derived polymers that have been extensively studied for various tissue engineering applications. This review discusses the recent advances, applications, and challenges of biological macromolecule-based scaffolds in urethral reconstruction.

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“…Urethroplasty with a buccal mucosal graft may be effective in severe cases or for patients who experience postoperative complications, but there are no generally accepted algorithms yet for the surgical management of hypospadias [1,[3][4][5]. Tissue engineering offers promising solutions to produce grafts that closely resemble the natural architecture of the urethra [6,7].…”

Section: Introductionmentioning

confidence: 99%

Trilayer composite scaffold for urethral reconstruction: in vitro evaluation of mechanical, biological, and angiogenic properties

Abbas,

Parangusan,

Yalcin

et al. 2024

Biomed. Mater.

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Regeneration of damaged urethral tissue remains a major challenge in the field of lower urinary tract reconstruction. To address this issue, various synthetic and natural biodegradable biomaterials are currently being explored for the fabrication of scaffolds that promote urethral regeneration and healing. In this study, we present an approach to fabricate a trilayer hybrid scaffold comprising a central layer of poly(lactic acid) (PLA) between two layers of chitosan. The chitosan/PLA/chitosan (CPC) scaffolds were fabricated by a sequential electrospinning process and their properties were evaluated for their suitability for urethral tissue engineering. The physical and biological properties of the CPC scaffolds were evaluated in comparison to electrospun PLA scaffolds and acellular dermis (Alloderm) as controls for a synthetic and a natural scaffold, respectively. Compared to the controls, the CPC scaffolds exhibited higher elastic modulus and ultimate tensile strength, while maintaining extensibility and suture retention strength appropriate for clinical use. The CPC scaffolds displayed significant hydrophilicity, which was associated with a higher water absorption capacity of the chitosan nanofibres. The degradation products of the CPC scaffolds did not exhibit cytotoxicity and promoted wound closure by fibroblasts in vitro. In addition, CPC scaffolds showed increased growth of smooth muscle cells, an essential component for functional regeneration of urethral tissue. Furthermore, in a chicken embryo-based assay, CPC scaffolds demonstrated significantly higher angiogenic potential, indicating their ability to promote vascularisation, a crucial aspect for successful urethral reconstruction. Overall, these results suggest that CPC hybrid scaffolds containing both natural and synthetic components offer significant advantages over conventional acellular or synthetic materials alone. CPC scaffolds show promise as potential candidates for further research into the reconstruction of the urethra in vivo.

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Tailor-made natural and synthetic grafts for precise urethral reconstruction

Cited by 8 publications

References 130 publications

Cell-Based Therapy for Urethral Regeneration: A Narrative Review and Future Perspectives

Cell-Based Therapy for Urethral Regeneration: A Narrative Review and Future Perspectives

Biological Macromolecule-Based Scaffolds for Urethra Reconstruction

Trilayer composite scaffold for urethral reconstruction: in vitro evaluation of mechanical, biological, and angiogenic properties

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