Application of Tilapia Skin Acellular Dermal Matrix to Induce Acute Skin Wound Repair in Rats

Abstract

Extracellular matrix (ECM) material with good biological activity is essential to simulate cell growth microenvironment, induce cell infiltration and angiogenesis, and promote the repair of large area acute skin wound. In this study, tilapia skin acellular dermal matrix (TADM) was prepared to simulate ECM microenvironment, which can promote substantial area acute wound healing in rats. The main component of TADM is type I collagen, which has good physical and chemical properties, biological activity and cell adhesion. TADM is a form of biomaterial with low immunogenicity, low risk of prion infection and lower economic cost than other related materials such as mammalian collagen biomaterials. Our results show that TADM can guide cell infiltration, angiogenesis, regulate the expression and secretion of inflammatory and skin repair correlated factors to promote tissue healing.

Decellularized tilapia fish skin: A novel candidate for tendon tissue engineering

“…Marine-derived acellular collagen scaffolds are emerging as attractive alternatives to traditional mammal-derived materials because they have similar structures and functions to the ECM, lower the risk of disease transmission, and are less likely to be contraindicated on religious grounds [ 21 ]. The scale of the fishery industry has rapidly increased, and approximately 70–85% of all products generated are waste or byproducts, including skin, scales, and skeletons [ 22 , 34 , 35 ]. Converting these unused resources into products that could aid economic or environmental development is of great interest.…”

“…By contrast, decellularized marine collagen scaffolds could overcome these disadvantages [ 21 ]. Decellularized tilapia fish skin (DTFS) has recently received considerable attention because it is easily obtained and processed, and displays excellent biosafety; moreover, its use is not contraindicated on ethical or religious grounds [ 20 , 22 ]. DTFS has been used successfully for burn treatment, bone regeneration, and wound healing [ 20 , 22 , 23 ].…”

“…Decellularized tilapia fish skin (DTFS) has recently received considerable attention because it is easily obtained and processed, and displays excellent biosafety; moreover, its use is not contraindicated on ethical or religious grounds [ 20 , 22 ]. DTFS has been used successfully for burn treatment, bone regeneration, and wound healing [ 20 , 22 , 23 ]. Besides, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide (EDC-NHS) is one of the most common crosslinking agents for collagen materials, offering a non-cytotoxic and zero length crosslinking option, since it is highly water soluble so that it could be easily and thoroughly removed by repeated post-crosslinking rinsing with water [ [24] , [25] , [26] ].…”

Preparation of Nile tilapia skin collagen powder by low-temperature and comprehensive evaluation of hemostasis and wound healing

“…Indicating that fish collagen powder does not cause intradermal irritation. 38 Subcutaneous implantation experiment. An excellent method for assessing the biodegradability and biocompatibility of the evaluated materials is subcutaneous implantation.…”

“…According to the obtained results, TSCP has a positive therapeutic activity on full-thickness skin wounds by inducing and promoting the invasive, and proliferative abilities of skin fibroblasts, significantly promoting wound contraction and healing, promoting skin structure reconstruction, preventing wound infection, encouraging angiogenesis, and promoting collagen deposition. 23,38 The potent biological activity makes TSCP a promising skin repair dressing.…”