Cryopreservation of Atlantic salmon <i>Salmo salar</i> sperm: effects on sperm physiology

Figueroa, Elías; Valdebenito, Iván; Merino, O.; Ubilla, Andrea; Risopatrón, Jennie; Farías, Jorge G.

doi:10.1111/jfb.13052

Cited by 60 publications

(45 citation statements)

References 75 publications

(152 reference statements)

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“…(2015b) in European Perch and Figueroa et al. () in Atlantic Salmon. Different cryoprotectant and cryopreservation method combinations did not have a significant effect on sperm motility as there was no significant difference in motility between the cryopreserved treatment groups.…”

Section: Discussionmentioning

confidence: 94%

Yellow Perch Sperm Motility, Cryopreservation, and Viability of Resulting Larvae and Juveniles

et al. 2018

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The sperm of Yellow Perch Perca flavescens of two different age-classes, age 0 and 3, were cryopreserved using two different cryoprotectants (dimethylsulfoxide [DMSO] and methanol [MetOH]) with two freezing methods (pellet and vial). The viability and quality of the progenies obtained from fertilization with cryopreserved sperm were then examined. The motility of Walleye Sander vitreus sperm was examined following cryopreservation and ultraviolet (UV) irradiation, with the aim of using heterologous cryopreserved sperm to inseminate Yellow Perch eggs to ensure gynogenesis. The first experiment compared the motility of fresh sperm and pellet method cryopreserved sperm devoid of salmon seminal plasma. Despite the high motility of fresh sperm-75% and 100%-of males age 0 and 3, respectively, the postthaw motility of seminal plasma devoid cryopreserved sperm was 0%. The second experiment addressed the efficiency of pellet and vial freezing methods with DMSO and MetOH supplemented with salmon seminal plasma. Cryopreserved sperm was thawed and motility measured. Sperm motility was not significantly different between pellet (13.3 ± 10.4%) and vial (10.3 ± 12.9%) methods in the absence of sperm extender; however, sperm motility of the pellet method was further improved (20 ± 8.7%) with the addition of sperm extender after thawing, while motility of the vial method sperm (10 ± 7.1%) was not. Cryopreserved sperm was further evaluated based on fertilization rate and ultimate survival and growth of larvae and juveniles through 14 d posthatch. Effects of UV exposure on fresh and pellet method cryopreserved sperm following UV irradiation were also examined. The motility of control sperm cryopreserved with DMSO and MetOH, and UV-exposed sperm cryopreserved with DMSO decreased from 100% motility before cryopreservation (fresh sperm) to 75% following cryopreservation, while UV-exposed sperm cryopreserved with MetOH decreased from 100% to 50%. This experiment provides significant new data to improve the effectiveness of straightforward cryopreservation techniques for Yellow Perch.Yellow Perch Perca flavescens is a North American game fish as well as an important species to aquaculturists. Advancements in artificial spawning, incubation and hatching of perch embryos, and larviculture over the past few decades have promoted the development of Yellow Perch aquaculture; however, several challenges and

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Section: Discussionmentioning

confidence: 94%

Yellow Perch Sperm Motility, Cryopreservation, and Viability of Resulting Larvae and Juveniles

et al. 2018

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“…Suquet et al (2016) reported 75% lower velocity in frozen/thawed spermatozoa of the great scallop compared to fresh. Linhart et al (2005) (Figueroa et al 2016), 97 µm s -1 to 134 µm s -1 in Prochilodus lineatus (Viveiros and Leal 2016;Viveiros et al 2017), 57 µm s -1 in rainbow trout Oncorhynchus mykiss (Suquet et al 2012b) and µm s -1 in Siberian sturgeon Acipenser baerii spermatozoa (Sarosiek et al 2014). VSL of sea urchin spermatozoa was shown to be 170-240 µm sec -1 in Hemicentrotus pulcherrimus (Hiramoto and Baba 1978) and 150-200 µm sec -1 in Paracentrotus lividus (Fabbrocini and D'Adamo 2017).…”

Section: Discussionmentioning

confidence: 97%

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

Demoy-Schneider¹,

Schmitt²,

Pennec

et al. 2018

Aquaculture

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High quality of sperm is essential to a high fertilization rate, especially post-cryopreservation. Assessment of sperm integrity, motility and energy reserves before cryopreservation is necessary for selection of milt with optimal fertilizing potential. We describe the effect of cryopreservation on the quality of black-lip pearl oyster, Pinctada margaritifera var. cumingii sperm. Evaluated quality indices of fresh and frozen/thawed P. margaritifera spermatozoa, included morphology, ultrastructure and motility characteristics relative to the energy content (ATP) and its capacity to be sustained by mitochondrial respiration. Morphology and ultrastructure were quantitatively evaluated using images obtained by optical microscopy assisted by the Image J software and TEM, respectively. Sperm motility was assessed using Image J software combined with a computer assisted sperm analysis plugin adapted for assessing P. margaritifera spermatozoa. Other sperm quality parameters evaluated included O 2 consumption, ATP content, and creatine kinase activity. Frozen/thawed spermatozoa exhibited damage to the head but retained a compact spherical shape. Sperm motility indicators showed a significant decrease in quality resulting from the freeze/thaw process. The percent of motile cells was 54% compared to 84% in fresh sperm, O 2 consumption was 4.8 compared to 44 nanomol min −1 , ATP content was 0.72 nmol/10 9 spermatozoa in the activating medium compared to 4.54 nmol/10 9 spermatozoa, and creatine kinase activity was 9.06 × 10 −5 IU mg −1 protein compared to 12.5 × 10 −5 IU mg −1 protein. The cryopreservation protocol allowed obtaining an acceptable motility rate after thawing, confirming the predictive value of sperm motility measurements before cryopreservation in terms of their ability to withstand freezing process. Highlights ► Quality of the frozen/thawed black-lip pearl oyster Pinctada margaritifera spermatozoa. ► Progress to an efficient cryoconservation method for P. margaritifera spermatozoa. ► Preservation of P. margaritifera through the durable development of the pearl oyster culture in French Polynesia.

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“…Mystus nemurus [26,38], African catfish Clarias gariepinus [35,[39][40][41][42][43], giant goramy Osphronemus goramy [44,45], lampam Barbonymus gonionotus [46,47], salmonid spermatozoa [48][49][50][51][52], common carp Cyprinus carpio [53][54][55][56][57][58], zebra fish, Danio rerio [59][60][61][62][63] Mozambique tilapia, Sarotherodon mossambicus [64,65], rainbow trout, Oncorhynchus mykiss [66][67][68][69][70][71], and seurukan Osteochillus vittatus [72]. However, cryopreservation of botia sperm has never been reported previously.…”

Section: Several Species Of Fish Sperm Have Been Successfully Cryoprementioning

confidence: 99%

The effect of combination of methanol with several concentration of honey solution on sperm quality of botia Chromobotia macracanthus Bleeker 1852 after short-term storage at −80 ℃

Abinawanto

Musthofa

Lestari

et al. 2019

Preprint

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Background: Clown loach or botia, Chromobotia macracanthus Bleeker 1852, is a popular freshwater ornamental fish. Lack of high quality broodstock and asynchronous gonad maturation are the main problem in breeding of this species. Cryopreservation is one of the methods to overcome this problem, and cryoprotectant plays a vital role in this process. Herein, we tested two types of cryoprotectants, namely methanol as a permeating cryoprotectant and honey solution as a natural non-permeating cryoprotectant. Hence, the objective of the present study was to determine best combination of honey solution and methanol for short-term storage of C. macracanthus sperm. Methods: One level of methanol (10%) combined with six levels of honey solution (0%, 0.1%, 0.3%, 0.5%, 0.7% and 0.9%) were tested in this study. Ringer’s solution was used as an extender. The diluted sperms were equilibrated for 25 min at 4 °C and then kept at −80 ℃ for 48 h. Sperms was thawed for 13 s at 40 °C. Spermatozoa viability, motility, and fertilization rates were then observed. Results: The one-way ANOVA showed that combination of methanol with several concentrations of honey had a significant effect on sperm viability and fertilization rates (P<0.05), but did have a significant effect on sperm motility (P>0.05). The study revealed that the 0.1% honey solution combined with 10% methanol resulted in the highest rates of motility (89.4 ± 5.45%), viability (85.75 ± 4.79 %), and fertilization (98.55 ± 1.69 %). Conclusion: A 0.1% honey solution combined with 10% methanol in Ringer’s was the best cryoprotectant for C. macracanthus spermatozoa preserved at -80 ℃.

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Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

Cited by 60 publications

References 75 publications

Yellow Perch Sperm Motility, Cryopreservation, and Viability of Resulting Larvae and Juveniles

Yellow Perch Sperm Motility, Cryopreservation, and Viability of Resulting Larvae and Juveniles

Quality assessment of cryopreserved black-lip pearl oyster Pinctada margaritifera spermatozoa

The effect of combination of methanol with several concentration of honey solution on sperm quality of botia Chromobotia macracanthus Bleeker 1852 after short-term storage at −80 ℃

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