Cryogenic preservation of sperm from lane snapper (Lutjanus synagris): Testing the effects of extenders and freezing rates on sperm quality

Gaitán‐Espitía, Juan Diego; Martínez-Silva, María Angélica; Borrero, Carlos E.; Ramirez, L.; Valencia, Juan P.

doi:10.1016/j.aquaculture.2012.12.002

Cited by 10 publications

(3 citation statements)

References 28 publications

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“…The sperm of lane snapper examined after cryoconservation process, (Riley et al, 2004), but in contrast to that reported for other species of lutjanidae (L. argentimaculatus) from 1.3 to 2.8 × 10 10 (Vuthiphandchai et al, 2009). For lane snapper the Colombian coast, sperm density reported was 1.3 × 10 9 cells mL -1 (Gaitán-Espitia et al, 2013). The variation in sperm density among different species of the same genus may be caused by differences in the geographic range or by sampling at different periods during the spawning season (Lanes et al, 2010).…”

Section: Discussionmentioning

confidence: 53%

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

et al. 2015

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This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min ), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C Criopreservação do sêmen do ariocó Lutjanus synagris (Linnaeus, 1758) ResumoEste estudo teve a finalidade de desenvolver e avaliar um protocolo de crioconservação do sêmen do ariocó Lutjanus synagris. Para caracterizar o sêmen foram avaliados a taxa de motilidade, a duração da motilidade, a concentração espermática e o espermatócrito. Em seis experimentos foram analisados os efeitos de três diluentes, com distintas composições iônicas e valores de pH distintos, combinados com sete concentrações de dimetilsulfóxido (0; 2,5; 5,0; 7,5; 10,0; 12,5 e 15,0%), cinco velocidades de congelamento (-110, -90, -60, -45 e -30°C/min), nove tempos de equilíbrio (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutos) e cinco proporções de sêmen:diluente (1:1; 1:3; 1:6; 1:10 e 1:20) sobre a taxa de motilidade e a duração da motilidade espermáticas. Posteriormente um teste de fertilização foi realizado para avaliar a viabilidade do sêmen crioconservado. O tratamento que propiciou maior taxa de motilidade e duração da motilidade espermáticas (P<0,05) foi aquele proporcionado pelo emprego do diluente com pH 8,2 com dimetilsulfóxido a 10%, em uma velocidade de congelamento de -60°C/min, com tempo de equilíbrio de 1 minuto e na proporção de 1:3 (v/v). O sêmen crioconservado apresentou taxa de fertilização superior a 69% validando o presente protocolo para o ariocó. A crioconservação do sêmen do ariocó é uma alternativa viável, sendo possível manter uma apropriada qualidade espermática.

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Section: Discussionmentioning

confidence: 53%

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

et al. 2015

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“…Commercial culture of snappers ( Lutjanus genus) is increasing worldwide and cryopreservation of spermatozoa is a vital procedure for facilitating the controlled reproduction of fish, overcoming some of the problems associated with broodstock supply (Vuthiphandchai et al, 2009). Research has been conducted in this fish group in order to develop protocols for cryopreservation of sperm in several species of this genus (Gaitán-Espitia et al, 2013). Some snappers ( Lutjanus analis ) are also listed as vulnerable by the IUCN and have been cultured in experimental farms in the Caribbean, Colombia and Brazil and protocols for sperm cryopreservation are under development (Sanches et al, 2013).…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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“…Sperm samples were stored at 4°C for 48 hr and diluted in 5% BTS™ (80% glucose, 12.7% sodium citrate, 2.7% EDTA, 2.7% NaHCO 3 , 1.5% KCl, 0.5% gentamycin sulphate; Beltsville Thawing Solution Minitüb™) and 10% DMSO (dimethyl sulfoxide) at a 1:4 ratio as recommended by Miliorini et al (2011) and Murgas et al (2007). Sperm was equilibrated for 15-20 min (Viveiros, Gonçalves, Nascimento, & Leal, 2015), drawn into sealed 0.5-ml straws, in triplicate, identified and maintained in a freezing equipment (IceCube® 14S) programmed with the cooling rates adapted from Gaitán-Espitia, Martínez-Silva, Borrero, Ramírez, and Valencia (2013) as follows: a slow cooling rate of 15°C/min (CR15) and a fast cooling rate of 30°C/ min (CR30) ( Table 1)…”

Section: Sperm Cryopreservation and Thawingmentioning

confidence: 99%

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

Paula

Murgas

Castro

et al. 2019

Reprod Domestic Animals

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This study aims to investigate the effect of different cooling rates on the semen cryopreservation of curimba (Prochilodus lineatus). Nineteen ejaculates were obtained from adults males and cryopreserved at 15°C/min (CR15), 30°C/min (CR30) (controlled temperature inside and outside straw, speed was stable during freezing) and direct freezing in liquid nitrogen vapour (~35.6°C/min) (CRNV). The straws were thawed and seminal parameters evaluated. DNA fragmentation through the comet assay was assessed. A fresh sperm sample was not frozen and used for analyses. Data were submitted to an analysis of variance (ANOVA), and means were compared by Scott–Knott test (p < 0.05) using the R Software. Mean motility percentage was 100%, and motility duration was 39.5 ± 5.7 s for the fresh sperm (subjective analysis); 58.9 ± 8.0% and 24.5 ± 5.7 s for CR15; 64.8 ± 4.8% and 26.5 ± 7.1 s for CR30; and 50.1 ± 16% and 25.7 ± 4.7 s for CRNV, respectively. Motility percentages were higher and equal between CR15 and CR30 compared to CRNV (p < 0.05). Some sperm motion kinetics, namely average path velocity (VAP) and straight line velocity (VAS), were higher for CR30 (p < 0.05), while curvilinear velocity (VCL) and velocity progression (PRO) were lower for CRNV (p < 0.05). Straightness (STR) and wobble (WOB) were the same among treatments (p > 0.05). Sperm morphology results indicated higher means for total morphological sperm alterations in CRNV. All cooling rates caused sperm DNA fragmentation, although CR30 provided a less harmful effect. This is the first report for cryopreserved P. lineatus sperm preserved under different controlled cooling rates. The cooling rate of 30°C/min is indicated for the cryopreservation of this fish sperm as it led to the lowest detrimental spermatozoa effects.

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Cryogenic preservation of sperm from lane snapper (Lutjanus synagris): Testing the effects of extenders and freezing rates on sperm quality

Cited by 10 publications

References 28 publications

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

Sperm cryopreservation of lane snapper Lutjanus synagris(Linnaeus, 1758)

Cryobanking of aquatic species

Effects of cooling rates on the quality of Prochilodus lineatus (Valenciennes, 1836) sperm

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