Cryoconservation and regeneration of axillary shoot meristems of Indigofera tinctoria (L.) by encapsulation–dehydration technique

Nair, Deepa S.; Reghunath, B. R.

doi:10.1007/s11627-009-9244-4

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…It also enables exchange of pathogen free propagules across international borders avoiding bulk transportation of plants, quarantine and spread of diseases (Bhanuprakash 2015). As the plant conversion ability is retained even after aseptic storage under culture room conditions (25 ± 2°C) or in liquid nitrogen (-196°C) it is being used for conservation of many endangered medicinal plant species (Nair and Reghunath 2009;Maqsood et al 2012, Gantait et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Calcium alginate encapsulated synthetic seed production in Plumbago rosea L. for germplasm exchange and distribution

Prakash

Nair

Alex

et al. 2018

Physiol Mol Biol Plants

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L. (Plumbaginaceae), is a medicinal shrub commercially exploited for its naphthoquinone principle, plumbagin, extracted from the roots especially for treating skin disorders. As the plant is exploited from the wild without being replenished, conservation of the species becomes inevitable. Synthetic seeds would provide for effective conservation, germplasm exchange and distribution of this species. A reliable protocol for synthetic seed production in has been developed encapsulating the axillary buds. The axillary buds from cultures established and multiplied using the nodal explants in Murashige and Skoog (MS) medium supplemented with Benzyl Adenine (BA) 1.5 mg/L and Indole 3-Acetic acid 1.0 mg/L, were used for synseed production. The plantlet conversion efficiency was the highest in synthetic seeds developed with sodium alginate 2.5% in modified MS with 0.4 M sucrose and CaCl 100 mM. This combination gave the earliest bud initiation (9.19 ± 0.39 days) and maximum number of shoots per explant (2.31 ± 0.16 shoots). Microshoots from the culture, when inoculated on to MS medium supplemented with Naphthalene Acetic Acid 1.0 mg/L gave the best rooting response with 10.67 ± 0.94 roots per plant and 5.42 ± 0.29 cm root length. This is the first report of synthetic seed production in using axillary buds as explant.

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Section: Introductionmentioning

confidence: 99%

Calcium alginate encapsulated synthetic seed production in Plumbago rosea L. for germplasm exchange and distribution

Prakash

Nair

Alex

et al. 2018

Physiol Mol Biol Plants

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“…Also effective, but to a lesser degree was the 1 mg/L IAA with 1 mg/L BA treatment, producing 36 ± 6.82 shoots per explants. In other related studies, proper combination of cytokinin and auxin formulations have shown to be critical for shoot elongation in many other plant species for their in vitro propagation (Rout et al, 2000;Karim et al, 2003;Skala and Wysokińska, 2004;Nair and Reghunath, 2009). The correct ratio of cytokinin and auxin is important as their interaction can promote the development of shoots (Kamat and Rao, 1978).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of different ratios of auxin and cytokinin for the in vitro propagation of Streptocarpus rexii Lindl.

North¹

2012

Int. J. Phys. Sci.

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A successful means of in vitro propagation of Streptocarpus rexii Lindl. using leaf tissue as the explants is described. Secondary explants were placed on several regeneration media supplemented with various concentrations of indole-acetic acid (IAA) and benzyladenine (BA). Optimal shoot proliferation was obtained on Murashige and Skoog basal media containing 0.1 mg/L IAA in combination with 0.5 mg/L BA. The growth of explants, as quantified by their fresh and dry weights, was significantly higher in the 1 mg/L IAA with 1 mg/L BA treatment as compared with the 0.1 mg/L IAA plus 1.0 mg/L BA. The increasing concentrations of BA reduced the percentage of explants forming roots. From regeneration media, shoots were transferred to Murashige and Skoog medium supplemented with 1 mg/L IAA for root induction. After six weeks, the rooted plantlets were removed from culture and successfully hardened off in the shade house. For the multiplication of S. rexii Lindl., the best combination was the one involving 1 mg/L IAA and BA. This treatment obtained a highest shoot count and had the highest growth rate. This could play a key role in the large scale mass propagation of this plant.

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“…The results show no hindrance in rooting of BAP-NAA induced shoots on I. potaninii. Reghunath (2009) andNair et al (2016) studied on I. tinctoria and noted that in vitro axillary shoot proliferation was achieved from single-node explants on MS medium containing various concentrations of 1 mg/L BA and IAA acid. Rajabudeen et al (2014) reported axillary buds of I. viscosa from nodal explants proliferated on 1 mg/L BAP and 1.5 mg/L NAA underwent multiplication of shoots.…”

Section: Discussionmentioning

confidence: 99%

“…A few in vitro culture studies have been reported for other Indigofera species like I. enneaphylla, I. potaninii, I. tinctoria, I. viscosa and I. tirta (Bharal and Rashid, 1978;Howell et al, 1986;Nair and Reghunath, 2009;Rajabudeen et al, 2014;Senthilkumar et al, 2015;Nair et al, 2016). All of them indicated that Indigofera species are highly recalcitrant.…”

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confidence: 99%

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

Özel¹,

Maesaroh²

2018

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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Indigofera zollingeriana is an important protein rich forage and dye plant species that grows widely in Indonesia. It is difficult to propagate the plant through seeds due to high dormancy. Therefore, there is need to develop both in vitro and ex vitro propagation techniques of I. zollingeriana. This study aimed to develop a protocol for multiple shoot regeneration of the plant under in vitro conditions using different combinations of BAP+0.01 mg/L NAA. The results showed that both epigeal and cotyledon node explants have high potential to regenerate with maximum number of regenerated shoots noted on the epigeal and the cotyledon node explants using MS medium containing 1.5 mg/L BAP + 0.01 mg/L of NAA and 1.0 mg/L BAP + 0.01 mg/L of NAA in the same order. The shoots regenerated on all combinations of BAP+NAA were rooted using 0.5 mg/L IBA that promoted rooting in effective manner. The rooted plants were easily acclimatized to external conditions in an environmental chamber. The results suggest that I. zollengriana plant species is no more difficult to regenerate under in vitro conditions. This research will aid in future studies related to breeding, propagation, biosynthesis and extraction of active compounds.

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Cryoconservation and regeneration of axillary shoot meristems of Indigofera tinctoria (L.) by encapsulation–dehydration technique

Cited by 13 publications

References 31 publications

Calcium alginate encapsulated synthetic seed production in Plumbago rosea L. for germplasm exchange and distribution

Calcium alginate encapsulated synthetic seed production in Plumbago rosea L. for germplasm exchange and distribution

Evaluation of different ratios of auxin and cytokinin for the in vitro propagation of Streptocarpus rexii Lindl.

Önemli bir ekvator yem bitkisi olan Indigofera zollingerian Miq.’nın çoklu sürgün rejenerasyonu

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