Evaluation of different ratios of auxin and cytokinin for the in vitro propagation of Streptocarpus rexii Lindl.

Abstract

A successful means of in vitro propagation of Streptocarpus rexii Lindl. using leaf tissue as the explants is described. Secondary explants were placed on several regeneration media supplemented with various concentrations of indole-acetic acid (IAA) and benzyladenine (BA). Optimal shoot proliferation was obtained on Murashige and Skoog basal media containing 0.1 mg/L IAA in combination with 0.5 mg/L BA. The growth of explants, as quantified by their fresh and dry weights, was significantly higher in the 1 mg/L IAA with 1 mg/L BA treatment as compared with the 0.1 mg/L IAA plus 1.0 mg/L BA. The increasing concentrations of BA reduced the percentage of explants forming roots. From regeneration media, shoots were transferred to Murashige and Skoog medium supplemented with 1 mg/L IAA for root induction. After six weeks, the rooted plantlets were removed from culture and successfully hardened off in the shade house. For the multiplication of S. rexii Lindl., the best combination was the one involving 1 mg/L IAA and BA. This treatment obtained a highest shoot count and had the highest growth rate. This could play a key role in the large scale mass propagation of this plant.

Organogenesis and Antioxidant Capacity of Streptocarpus ×hybridus In Vitro under Different LED Light Spectrum Composition

“…A shoot height at a statistically high level was also obtained under the influence of red light (R-5.83), red with blue (RB-5.33), the addition of green (RBG-5.47) LED and under fluorescence (Fl-6.53). In the studies carried out to date on plant in vitro cultures of Streptocarpus by various authors, there have been no reported studies that have examined the effect of different quality of LED light on its developmental parameters [13,[35][36][37]. Moreover, as for the in- In the studies carried out to date on plant in vitro cultures of Streptocarpus by various authors, there have been no reported studies that have examined the effect of different quality of LED light on its developmental parameters [13,[35][36][37].…”

“…In the studies carried out to date on plant in vitro cultures of Streptocarpus by various authors, there have been no reported studies that have examined the effect of different quality of LED light on its developmental parameters [13,[35][36][37]. Moreover, as for the in- In the studies carried out to date on plant in vitro cultures of Streptocarpus by various authors, there have been no reported studies that have examined the effect of different quality of LED light on its developmental parameters [13,[35][36][37]. Moreover, as for the influence of light alone on the biometrics of proliferated plants of different species, the majority of analyses carried out by various authors concern the effect of red and blue LED light and their combinations in various proportions ( [21,[38][39][40][41][42] and many others).…”