The aim of this study was to determine the response of the growth of internode explants and leaves of Stevia (Stevia Rebaudiana Bertoni), which were cultured at several BAP concentrations. This study was designed using a non Factorial Complete Randomized Design that is by testing the internode and leaf explants at 3 different BAP concentration levels of 2, 3 and 4 ppm and will be repeated 10 times. The parameters observed related to callus growth include: emergence, type, color and percentage of callus formation. In addition, observations were also made on the parameters of bud growth including the appearance of shoots, number and length of shoots. The results of this study indicate that 100% of leaf and internode explants are able to form callus. The color of the callus explants on average leaves are yellowish green while the internode explants are yellowish white and all have compact textures. In shoot growth parameters, only internode explants were able to grow shoots while leaf explants were not. The emergence of shoots (3 DAP), number of shoots (8.70), and shoot length (2.81cm) were produced by MS media with the addition of 3ppm BAP.
Tujuan penelitian ini untuk mengetahui mengenai multiplikasi tunas tanaman kapas (gossypium spp.) varietas Kanesia 15 menggunakan kombinasi BAP dan NAA secara in vitro. Penelitian ini dilaksanakan pada bulan Oktober 2017 Sampai Dengan Januari 2018 bertempat di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial yang terdiri atas 2 faktor dengan 4 ulangan. Faktor pertama adalah BAP (B) yaitu B1= 0,00 ppm, B2= 1,50 ppm, B3= 3,00 ppm, dan B4= 4,50 ppm. Faktor kedua adalah zat pengatur tumbuh NAA (N) yaitu N1= 0,00 ppm, N2= 0,10 ppm, dan N3= 0,50 ppm. Data pengamatan yang diperoleh, diuji menggunakan Analysis of Varians (ANOVA), jika berpengaruh nyata dilanjutkan dengan uji lanjut Duncan Multiple Range Test (DMRT) pada taraf 5%. Hasil penelitian menunjukkan bahwa pemberian BAP pada media memberikan pengaruh yang berbeda tidak nyata terhadap kedinian tunas, jumlah tunas, dan diameter eksplan, namun memberikan pengaruh yang sangat nyata terhadap tinggi eksplan, jumlah ruas, jumlah akar, dan panjang akar.
Penelitian ini bertujuan untuk mengetahui konsentrasi 2,4-D yang tepat untuk menumbuhkan kalus pada beberapa kultivar tanaman kentang dataran medium seperti DTO-28 dan Desiree. Penelitian ini menggunakan Rancangan Acak Lengkap faktorial. Faktor yang dicoba adalah konsentrasi 2,4-D: 4,53; 9,05; 13,58; 18,10 μM dan Kultivar Kentang: DTO-28 dan Desiree. Terdapat total 8 perlakuan dengan 5 kali ulangan. Parameter yang diukur meliputi saat muncul kalus, berat segar kalus, persentase terbentuknya kalus, serta parameter kualitif terhadap morfologi kalus, seperti tekstur, warna, jenis kalus, jumlah tunas dan akar. Data hasil pengamatan terhadap morfologi kalus disajikan secara deskriptif, sedangkan data yang lain dianalisis dengan analisis ragam (ANOVA). Untuk mengetahui perbedaan antara perlakuan dilakukan uji DMRT pada taraf 5%.Hasil penelitian (33 HST) menunjukkan bahwa semua semua eksplan dari Kultivar DTO-28 dan Desiree mampu membentuk kalus, dengan tekstur remah berair dan rata-rata berwarna putih dan hijau kekuningan. Kultivar DTO-28 lebih responsif terhadap ZPT 2,4-D dan mampu memunculkan kalus lebih cepat yaitu pada 5 HST. Kedua kultivar yang diujikan mampu menghasilkan kalus terberat pada konsentrasi 18,10 μM 2,4-D.Dari hasil tersebut dapat disimpulkan bahwa konsentrasi 18,10 μM 2,4-D merupakan konsentrasi terbaik dalam memacu pertumbuhan kalus kentang DTO-28 dan Desiree.
Penelitian ini bertujuan mengetahui pengaruh penggunaan beberapa jenis sitokinin dan beberapa levelkonsentrasi air kelapa terhadap respon pertumbuhan tunas mikro stevia. Rancangan Penelitian ini menggunakanRancangan Acak Lengkap (RAL), meliputi 3 jenis sitokinin (2 ppm Kinetin, 2ppm BAP, 2ppm TDZ) dan 4 levelkonsentrasi air kelapa (0%; 5%; 10%; 15%) dengan 5 ulangan. Parameter pengamatan meliputi persentasekontaminasi, persentase browning, saat muncul tunas, jumlah tunas, panjang tunas dan jumlah ruas. Data dianalisisdengan analisis ragam (ANOVA) dan uji DMRT pada taraf 5%. Hasil penelitian pada 30 HST untuk parametersaat muncul tunas, jumlah tunas, panjang tunas dan jumlah ruas, interaksi antara BAP dan air kelapa menunjukkanperbedaan yang tidak nyata. Namun hasil analisis menunjukkan pengaruh tunggal sitokinin dan air kelapa dalampembentukan dan pertumbuhan tunas. Media perlakuan dengan menggunakan MS+2ppm BAP tanpa air kelapaterbukti memacu kemunculan tunas lebih cepat, yaitu rata-rata 3,05 Hari Setelah Kultur (HSK) serta memacupertambahan jumlah ruas (2,6 ruas per tunas) dan tunas (3,7 tunas per eksplan). Penambahan air kelapamenghambat kecepatan pertumbuhan tunas. Parameter pertumbuhan tunas menunjukkan semakin besarkonsentrasi air kelapa yang ditambahkan semakin menurunkan pertumbuhan tunas baik dari jumlah, panjang atauruas.Kata kunci: Stevia, in vitro, air kelapa, sitokininABSTRACTThis study aimed to determine effect of use of several types of cytokinin and levels of coconut waterconcentration on stevia micro shoot growth response. This study was arranged in a Randomized Block Design,including 3 types of cytokines (2 ppm Kinetin, 2ppm BAP, 2ppm TDZ) and 4 levels of coconut water concentration(0%, 5%, 10%, 15%) with 5 replications. The parameters included percentage of contamination, percentage ofbrowning, time to form shoots, number of shoots, shoot length and number of nodes. Analyzed by variance analysis(ANOVA) and DMRT test at 5% level. For analysis results of time to form shoots, number of shoots, shoot lengthand number of segments showed that the interaction between BAP and coconut water gave no significantdifference. However, the results of the analysis showed a single effect of cytokinin and coconut water in shootformation and growth. Treatment media using MS + 2ppm BAP without coconut water proved to accelerate theappearance of shoot more rapidly, that is average 3.05 days after culture and induced the increase of the numberof node (2.6 node per shoot) and shoot number (3.7 shoots per explant). The addition of coconut water inhibitedthe growth rate of buds. Shoot growth of the number, length, and node number of shoots decline due to highconcentration of coconut water added further decrease the growth of shoot either from the number, length, andnode number of shoots.Key words: Stevia, in vitro, coconut water, cytokinins
Indonesia is one of the centers of durian diversity in the world and one of the regions that makes durian a superior local fruit crop is Jember and Banyuwangi. Clustering is useful to facilitate the search for certain features of the type of durian. This study aims to determine the durian grouping in two districts namely Jember and Banyuwangi. The materials used were local superior durian fruit and characterization questionnaire. Research using descriptive methods. Characterization data will be analyzed using NTSYS software.The results showed that local superior durian fruit were grouped into 2 groups based on morphological characters. Cluster 1 consists of 4 durians namely Klemben, Hijau, Bajul, and Lambau. Cluster 2 only consists of durian Musang King. Based on the chemical content of durian fruit, durian Lambau from Jember has the highest water content of 75.52%, Musang King from Banyuwangi has a fat content (2.29%), Bajul from Jember has the highest fiber content (11.89%), and Hijau from Banyuwangi has the highest protein (3.14%) and sugar (26.08%) content. Based on PCA showed that Musang King and Hijau have similirity on some morphological characters and chemical fruit contents. The results of correlation analysis of morphological characters and chemical content of durian fruit showed that leaf length was positively correlated with leaf width and protein content. Leaf width is positively correlated with sugar content. Fruit weight is positively correlated with water content.
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