Cryoalgotox: Use of cryopreserved alga in a semistatic microplate test

Benhra, Ali; Radetski, Claudemir M.; Férard, Jean-François

doi:10.1002/etc.5620160316

Cited by 10 publications

(4 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Toxicity results obtained with unadjusted pH were hardly consistent with the pure metal toxicity data found in the literature. In fact, the copper 72-h EC50 value reported by Benhra et al [31] was 28.5 g/L. Comparing this value to the copper concentration in the leachate (891 g/L), the maximum dilution factor able to induce an EC50 is 31.…”

Section: Discussionmentioning

confidence: 88%

Ecotoxicological assessment of solid wastes: A combined liquid‐ and solid‐phase testing approach using a battery of bioassays and biomarkers

Ferrari¹,

Radetski²,

Veber³

et al. 1999

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

Abstract-Municipal solid waste incinerator bottom ash (MSWIBA) was used as a test matrix for comparing the responses of different variables (solid phase vs leachate media) through toxicity tests and resulting endpoints. Toxicity of leachate and solidphase MSWIBA was evaluated with three terrestrial plants, namely oats (Avena sativa L.), Chinese cabbage (Brassica campestris L. cv. chinensis), and lettuce (Lactuca sativa L.). Assessment endpoints for these plant tests were biomass fresh weight, germination rate, and oxidant stress enzyme activities (superoxide dismutase, catalase, peroxidase, and glutathione reductase). Leachate MSWIBA toxicity was tested with bacteria (Vibrio fischeri), algae (Pseudokirchneriella subcapitata), and microcrustaceans (Daphnia magna and Ceriodaphnia dubia). A large range of toxicity responses was obtained with the various bioassays. With plant tests, the phytotoxicity of MSWIBA was revealed by the solid-phase approach, whereas no toxicity was observed with the MSWIBA leachate. The increase of oxidant stress enzyme activities was demonstrated to be a good indicator of solid or leachate phase toxicity.

show abstract

Section: Discussionmentioning

confidence: 88%

Ecotoxicological assessment of solid wastes: A combined liquid‐ and solid‐phase testing approach using a battery of bioassays and biomarkers

Ferrari¹,

Radetski²,

Veber³

et al. 1999

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…Conservation of endangered species, DNA or germaplasm cryobanking of marine organisms additional applications (Odintsova et al 2001, Hagedorn et al 2006, Rhodes et al 2006, Hagedorn et al 2014, Odintsova and Boroda 2012, Paredes et al 2013, Ohki et al 2014. Cryopreservation of marine organisms/cells has also been applied to ecotoxicology, as embryo-larval bioassays are a very widespread and useful tool for marine water toxicity assessment (Grout and McFadzen 1991, McFadzen 1992, McFadzen and Clearly 1994, Benhra et al 1997, Paredes and Bellas 2009, Bellas and Paredes 2011, Fabbrocini et al 2012.…”

Section: Facts Trends and Constraintsmentioning

confidence: 99%

Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

Paredes

2015

Cryobiology

View full text Add to dashboard Cite

“…Use of cryopreserved freshwater algae Selenastrum capricornutum in ecotoxicity testing has been evaluated by Benhra et al (1997). Experiments compared the performance of this method, named Cryoalgotox, versus the classic microplate test using fresh algae.…”

Section: Cryopreserved Microalgaementioning

confidence: 99%

The Use of Cryopreserved Biological Material for Water Quality Assessment

Paredes

Bellas

2019

Front. Mar. Sci.

View full text Add to dashboard Cite

The stated aim of this perspective article is to present new developments and discuss future directions on the applications of cryopreserved organisms to marine water quality assessment. To facilitate this, the authors provide a background of essential knowledge of cryopreservation when applied to ecotoxicology, as well as, practical examples available in literature. An integrated approach with combined monitoring of chemical status plus measurements of biological effects has been recommended extensively by international institutions for the assessment of marine pollution. Among the available techniques, bioassays have been considered as sufficiently robust to be incorporated in marine pollution monitoring programs. However, the routine application of bioassays has also allowed the identification of one of the factors that limits a more extensive use of such biological methods: the availability of biological material throughout the year, regardless of natural spawning periods. A solution to this limitation is the application of cryopreservation techniques. Cryopreservation may, for instance, provide access to stable quality biological material when test species are out of the reproductive season, without the need for maintaining and conditioning organisms in the laboratory. It also guarantees access to a large variety of species that might not be available at the same time of the year and, on top of that, cryopreservation provides opportunities to laboratories that might not have the facilities to keep all these organisms in culture.

show abstract

Cryoalgotox: Use of cryopreserved alga in a semistatic microplate test

Cited by 10 publications

References 9 publications

Ecotoxicological assessment of solid wastes: A combined liquid‐ and solid‐phase testing approach using a battery of bioassays and biomarkers

Ecotoxicological assessment of solid wastes: A combined liquid‐ and solid‐phase testing approach using a battery of bioassays and biomarkers

Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

The Use of Cryopreserved Biological Material for Water Quality Assessment

Contact Info

Product

Resources

About