Ecotoxicological assessment of solid wastes: A combined liquid‐ and solid‐phase testing approach using a battery of bioassays and biomarkers

Ferrari, B.; Radetski, Claudemir M.; Veber, Anne-Marie; Férard, Jean-François

doi:10.1002/etc.5620180618

Cited by 42 publications

(47 citation statements)

References 33 publications

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“…Li et al [54] and Bakare et al [55] reported that the possible mechanism of municipal landfill and sludge leachates induced genotoxicity and toxicity in mice was by oxidative damage. Similarly, oxidative stress was implicated in incinerated bottom ash and municipal landfill leachates induced toxicity and genotoxicity in plant test systems [56][57][58]. These are in concert with our findings that e-waste leachate and contaminated well water induced elevated levels of lipid peroxidation and alterations in oxidative stress enzymes in liver of treated mice.…”

Section: Discussionsupporting

confidence: 88%

In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water

et al. 2013

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Environmental, plant and animal exposure to hazardous substances from electronic wastes (e-wastes) in Nigeria is increasing. In this study, the potential cytogenotoxicity of e-wastes leachate and contaminated well water samples obtained from Alaba International Electronic Market in Lagos, Nigeria, using induction of chromosome and root growth anomalies in Allium cepa, and micronucleus (MN) in peripheral erythrocytes of Clarias gariepinus, was evaluated. The possible cause of DNA damage via the assessments of liver malondialdehyde (MDA), catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD) as indicators of oxidative stress in mice was also investigated. There was significant (p < 0.05) inhibition of root growth and mitosis in A. cepa. Cytological aberrations such as spindle disturbance, C-mitosis and binucleated cells, and morphological alterations like tumor and twisting roots were also induced. There was concentration-dependent, significant (p < 0.05) induction of micronucleated erythrocytes and nuclear abnormalities such as blebbed nuclei and binucleated erythrocytes in C. gariepinus. A significant increase (p < 0.001) in CAT, GSH and MDA with concomitant decrease in SOD concentrations were observed in the treated mice. Pb, As, Cu, Cr, and Cd analyzed in the tested samples contributed significantly to these observations. This shows that the well water samples and leachate contained substances capable of inducing somatic OPEN ACCESSChallenges 2013, 4 170 mutation and oxidative stress in living cells; and this is of health importance in countries with risk of e-wastes exposure.

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Section: Discussionsupporting

confidence: 88%

In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water

et al. 2013

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“…Nickel, which has been found to be highly toxic, was also analysed in the sample at levels much higher than in control and maximum permissible limits for leachate discharged by regulatory bodies [17]. Nickel is known to penetrate cells rapidly and causes osmotic lyses of cells resulting in cell death [26].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Cytogenotoxicity of Electronic Waste Leachate from Iloabuchi Electronic Market, Diobu, Rivers State, Nigeria on Allium Cepa

Babatunde

Anabuike

2015

Challenges

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Abstract:The human and environmental impact of electronic waste is increasing due to its careless disposal. Cytogenotoxicity of electronic waste from Iloabuchi electronic market, Diobu, Rivers State was investigated using the Allium cepa bioassay comprised of the root elongation and chromosome aberration tests. Leachate samples of e-waste analysed were above maximum permissible limits. Toxicity to root growth of A. cepa was evaluated at concentrations of 5%, 10%, 25%, 50%, and 100% showed root growth inhibition at all concentrations of the samples compared to the control and root growth inhibition was concentration dependent. An effective concentration (EC50) at which root growth amounted to 50 % of control for the sample was 37.5%. Various morphological defects of the onion roots were observed including short, crochet roots, C-tumor roots and severe toxic effects where no growth was observed. In the in vivo genotoxity assay, all samples lowered the frequency of mitotic cells in the meristematic region of the roots at statistically significant levels (P < 0.05) compared to the control and mitotic inhibition was also concentration dependent. There was significant induction of aberrations at all concentrations tested compared to control. The high metal content of the e-waste leachate may be responsible for observed cytotoxicity in A. cepa roots cells.

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“…Ten percent leachates were prepared from each of the three solid waste samples by inorganic-aqueous extraction using the French standard method [Ferrari et al, 1999], with minor modifications. Briefly, 100 g of solid waste were added to 1000 ml of demineralized water (w/v), and this mixture was shaken continuously for 24 hr at ambient room temperature (25 6 18C).…”

Section: Preparation Of Leachatesmentioning

confidence: 99%

In vivo genotoxic effects of industrial waste leachates in mice following oral exposure

Chandra

Chauhan

Dhawan

et al. 2006

Environ. Mol. Mutagen.

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Contamination of ground water by industrial waste poses potential health hazards for man and his environment. The improper disposal of toxic wastes could allow genotoxic chemicals to percolate into ground waters, and these contaminated ground waters may produce toxicity, including mutation and eventually cancer, in exposed individuals. In the present study, we evaluated the in vivo genotoxic potential of leachates made from three different kinds of industrial waste (tannery waste, metal-based waste, and waste containing dyes and pigments) that are disposed of in areas adjoining human habitation. Three different doses of test leachates were administered by oral gavage for 15 consecutive days to Swiss albino mice; their bone marrow cells were examined for chromosome aberrations (CAs), micronucleated polychromatic erythrocytes (MNPCEs), and DNA damage using the alkaline Comet assay. Exposure to the leachates resulted in significant (P < 0.05 or P < 0.001) dose-dependent increases in chromosome and DNA damage. Fragmented chromosomes and chromatid breaks were the major CAs observed. Chemical analysis of the leachates indicated that chromium and nickel were elevated above the limits established by health organizations. The highest levels of genotoxicity were produced by the metal-based leachate and the tannery-waste leachate, while the dye-waste leachate produced weaker genotoxic responses. The cytogenetic abnormalities and DNA damage produced by the leachates indicate that humans consuming water contaminated with these materials are at increased risk of developing adverse health consequences.

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Ecotoxicological assessment of solid wastes: A combined liquid‐ and solid‐phase testing approach using a battery of bioassays and biomarkers

Cited by 42 publications

References 33 publications

In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water

In Vivo Cytogenotoxicity and Oxidative Stress Induced by Electronic Waste Leachate and Contaminated Well Water

In Vivo Cytogenotoxicity of Electronic Waste Leachate from Iloabuchi Electronic Market, Diobu, Rivers State, Nigeria on Allium Cepa

In vivo genotoxic effects of industrial waste leachates in mice following oral exposure

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