Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

Paredes, Estefanía

doi:10.1016/j.cryobiol.2015.08.011

Cited by 36 publications

(48 citation statements)

References 94 publications

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“…Among marine invertebrates, the edible oysters are most widely studied [36], but 95% of the work has focused on broadcasting species [18]. The post-thaw sperm motility achieved in spermcasting species is low [21,39] compared to broadcasting species [13,41] probably because of the difference in their spermatological characteristics.…”

Section: Introductionmentioning

confidence: 99%

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Hassan

Qin

2017

Cryobiology

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Low survival of cryopreserved sperm impedes the application of cryopreservation technique in spermcasting oyster species. This study developed a simple method of liquid nitrogen vapor freezing to improve post-thaw sperm survival in the spermcasting oyster Ostrea angasi. The results indicate that the permeable cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG) and propylene glycol (PG) were non-toxic to sperm up to 20% concentration and 90 min exposure whereas methanol at 10% or higher was toxic to sperm for any exposure over 30 min. Among the treatments with permeable cryoprotectants, 15% EG produced the highest post-thaw sperm motility. Sperm motility was further improved by the addition of non-permeable cryoprotectants (trehalose and glucose), with 15% EG + 0.2 M trehalose resulting in the highest post-thaw sperm motility among all the combinations evaluated. The durations of 20, 30 and 60 min equilibrations produced a higher post-thaw sperm motility and plasma membrane integrity (PMI) than 10 min. Higher post-thaw motility and PMI were achieved by freezing sperm at the 8 cm height from the liquid nitrogen surface than at the 2, 4, 6, 10 or 12 cm height. Holding sperm for 10 min in liquid nitrogen vapor produced higher post-thaw motility and PMI than for 2, 5 or 20 min. The cryopreservation protocol developed in this study improved both post-thaw motility and PMI of O. angasi sperm at least 15% higher than those cryopreserved using programmable freezing method. Liquid nitrogen vapor freezing might have greater applicability in improving post-thaw sperm quality of spermcasting oyster species.

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Section: Introductionmentioning

confidence: 99%

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Hassan

Qin

2017

Cryobiology

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“…Currently, DMSO is recognized as the most successful CPA out of many CPAs tested in various studies published on marine species (Liu et al, ; Paredes, ), because it offers the highest post‐freeze survival reputation. In this study, the results indicated that the cryoprotectant DMSO may be more suitable for Chlamys nobilis sperm programmable cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

Sperm cryopreservation of the noble Scallop Chlamys nobilis by a programmable freezing method: Effect of cryoprotectant

et al. 2019

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A study on Chlamys nobilis sperm cryopreservation by a programmable freezing method was conducted under laboratory condition. Four cryoprotectant agents (dimethyl sulfoxide [DMSO], methanol [MET], propanediol[PG] and ethylene glycol [EG]) and four concentrations (5%, 10%, 20% and 30%) were evaluated for their ability to retain sperm motility, movement characteristics and fertility. Results showed that cryopreserved sperm total motility produced by DMSO and MET at 5%, 10% and 20% were higher than other cryoprotectant treatment groups (CPA groups), as well as rapid sperm percentage. The curvilinear (VCL) and straight line (VSL) velocity produced by DMSO at 5% significantly higher than other CPA groups (p < 0.05), while no significant differences were found for average path (VAP) velocity. The lateral head displacement (ALH) in all CPA groups was similar and without significant difference (p > 0.05), as well as the beat‐cross frequency (BCF). A significant higher fertilization rate was produced in DMSO than that in MET at same concentration (p < 0.05), and no significant differences were found for differing concentrations of the same cryoprotectant (p > 0.05). Overall, 5%‐20% DMSO was more suitable for Chlamys nobilis sperm programmable cryopreservation when the calcium‐free Hanks’ balanced salt solution was used as the extender, and 10°C/min from 0°C to −80°C was used as freezing rate. The findings presented in this study will benefit conservation programs for Chlamys nobilis.

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“…Last but not least, it also aligns with the 3R's of animal welfare principle of reduction, by allowing the storage of unused material for other experiments therefore reducing the number of animals used per trial. As more marine organisms have been successfully cryopreserved, including different cells or development stages, there is great potential for this to continue to develop (Suquet et al, 2000;Paredes, 2015b).…”

Section: Discussionmentioning

confidence: 99%

“…Many of the microalgae currently held in culture collections are kept cryopreserved, there are also available protocols for different molluscs (Wang et al, 2011;Paredes et al, 2013) and being mussels the most widely used organism for biomonitoring, this is another potential candidate for the development of a cryopreserved toxicity test in the near future. Regarding sea urchins, right now there are cryopreservation protocols developed or under development for different cells for 10-14 different sea urchin species, and being sea urchins a highly demanded model soon more applications will be developed, including toxicology (Paredes, 2015b).…”

Section: Discussionmentioning

confidence: 99%

The Use of Cryopreserved Biological Material for Water Quality Assessment

Paredes

Bellas

2019

Front. Mar. Sci.

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The stated aim of this perspective article is to present new developments and discuss future directions on the applications of cryopreserved organisms to marine water quality assessment. To facilitate this, the authors provide a background of essential knowledge of cryopreservation when applied to ecotoxicology, as well as, practical examples available in literature. An integrated approach with combined monitoring of chemical status plus measurements of biological effects has been recommended extensively by international institutions for the assessment of marine pollution. Among the available techniques, bioassays have been considered as sufficiently robust to be incorporated in marine pollution monitoring programs. However, the routine application of bioassays has also allowed the identification of one of the factors that limits a more extensive use of such biological methods: the availability of biological material throughout the year, regardless of natural spawning periods. A solution to this limitation is the application of cryopreservation techniques. Cryopreservation may, for instance, provide access to stable quality biological material when test species are out of the reproductive season, without the need for maintaining and conditioning organisms in the laboratory. It also guarantees access to a large variety of species that might not be available at the same time of the year and, on top of that, cryopreservation provides opportunities to laboratories that might not have the facilities to keep all these organisms in culture.

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Exploring the evolution of marine invertebrate cryopreservation – Landmarks, state of the art and future lines of research

Cited by 36 publications

References 94 publications

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Improvement of post-thaw sperm survivals using liquid nitrogen vapor in a spermcasting oyster Ostrea angasi

Sperm cryopreservation of the noble Scallop Chlamys nobilis by a programmable freezing method: Effect of cryoprotectant

The Use of Cryopreserved Biological Material for Water Quality Assessment

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