Cryo-Immunology: A Method of Immunization to Autologous Tissue.

Clinical and Experimental Considerations of the Immunologic Response to Prostatic and Other Accessory Glands Tissues of Reproduction

“…Control rabbits were treated in the same manner, but without freezing. Previous reports have described the surgical procedure and temperature measurements in detail [81][82][83]. Tissues subjected to freezing were the coagulating gland and seminal vesicles.…”

“…After the 10th day, there was a steady decline in the titre. Rabbits which were not frozen, but were sham-operated as controls, all showed negative results [81][82][83]. These antibodies were shown to be autoantibodies by virtue of positive reactions obtained in testing sera of the animals which had undergone cryosurgery with extract preparations of their own accessory glands tissue [81][82][83].…”

“…These sera also showed no reactivity when tested with human, guinea-pig, or rat accessory glands tissue. Similar freezing of the kidney failed to lead to any subsequent serum activity against extracts of accessory glands tissue [81][82][83].…”

Cryoimmunotherapy: Continuing Studies toward Determining a Rational Approach for Assessing the Candidacy of the Prostatic Cancer Patient for Cryoimmunotherapy and Postoperative Responsiveness

“…This in situ freezing of tissue constitutes an antigenic stimulus (comparable to that obtained through the parenteral administration of antigen ) which is capable of generating a specific immunologic response against autolo gous antigens of the frozen tissue (37), ergo, cryostimulation.…”

Cryoimmunotherapy: a Conference Report

“…The application of cryosurgery to a variety of normal and pathologic tissues has demonstrated that in situ freezing of many tissues constitutes an antigenic stimulus (comparable to that obtained through the parenteral administration of antigen), capable of generating a specific immunologic response to autologous antigens of the frozen tissue, ergo, cryostimulation [19]. Interest in the phenomenon of cryostimulation, spurred in part by reports of secondary tumor cell destruction beyond the freezing site, i.e., of distant metastases [8,12,20,21], has prompted continuing investigation into the applicability of cryosurgery, not only for destruction of primary tumor, but also for augmentation of induction of host resistance to tumor, ergo, cryoimmunotherapy [2], However, if cryosurgery is to be efficaciously employed not only for ablation of tumor [4,17], but also as a possible means of engendering an immunologic response to tumor (primary as well as secondary, i.e., metastases) [8], several factors must be given careful consideration [1], Evolving from initial experimental studies [10], these factors have been referred to as the 'cryosensitivity' of the host [5], and may tentatively and generally said to be related, as outlined in table I, to: (i) the immunogenicity of the target tissue, i.e., the tissue frozen, and (ii) the immunocompetency of the host.…”