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Sedimentation and diffusion measurements have been made on solutions of bovine fibrinogen, using several experimental methods. The resulting sedimentation constant, extrapolated to infinite dilution and corrected to standard conditions, was 7.80 X 10-12 sec.-1 in the Spinco ultracentrifuge and 7.95 X 10-1* sec.-1 in the oil-turbine ultracentrifuge. The diffusion constant at a concentration of 4.0 g./l. was 2.02 X 10-7 cm.2 sec.-1, using a schlieren optical system, and 2.01 X 10-7 cm.2 sec.-1, employing a Gouy interferometer optical system. Appropriate combination of these results gives a molecular weight of 330,000 ± 10,000 and a frictional ratio of 2.34. The intrinsic viscosity was found to be 0.25. Deductions as to the molecular shape and dimensions have been made by use of several hydrodynamic theories.(1) This is Paper No. 26 of a series on ''The Formation of Fibrin and the Coagulation of Blood*' from the University of Wisconsin, sup*

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