Preliminary gel diffusion precipitation studies of rabbit antisera to saline extracts of pooled normal human prostatic tissue have demonstrated the presence of three prostate-specific antigens. Two of the antigens were found in prostatic tissue extract, and the third occurred in both prostatic tissue extract and prostatic fluid. Several studies initiated in this laboratory have dealt with the immunological and immunochemical characterization of normal and pathological human prostatic fluid (Soanes, Gabrielli & Felch, 1961 ; Shulman, Bronson, Gonder &
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