Cry-Consensus Peptide, a Novel Peptide for Immunotherapy of Japanese Cedar Pollinosis, Induces Th1-Predominant Response in Cry j 1-Sensitized B10.S Mice

Kozutsumi, Daisuke; Tsunematsu, Miwako; Yamaji, Taketo; Murakami, Rika; Yokoyama, Minehiko; Kino, Kohsuke

doi:10.1248/bpb.29.2506

Cited by 9 publications

(8 citation statements)

References 45 publications

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“…Kozutsumi et al studied the effect of Japanese cedar pollinosis Cry-consensus peptide (CCP) on Cry j1-specific TH1/TH2 responses using B10.S as a mice model. Corroborating with our results, CCP-treated (subcutaneously) mice exhibited increase in Cry j1-specific IgG2a antibodies, decreased IgG1 and IgE production, and increased secretion of IFN-γ cytokine by splenocytes (43). Similarly, treatment of PLA2 (a major bee venom allergen)-sensitized mice with intraperitoneal injections of a mixture of three overlapping peptides (44~60 mer) spanning the entire PLA2 molecule (100 µg/peptide) induced a sharp drop in PLA2-specific IgE antibodies, an increase in specific IgG2a antibodies with T cell cytokine secretion shifting from a TH2 to TH1 profile (44).…”

Section: Discussionsupporting

confidence: 89%

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

et al. 2009

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BackgroundThe present study examines a hypothesis that short allergen-derived peptides may shift an Aspergillus fumigatus (Afu-) specific TH2 response towards a protective TH1. Five overlapping peptides (P1-P5) derived from Asp f1, a major allergen/antigen of Afu, were evaluated for prophylactic or therapeutic efficacy in BALB/c mice.MethodsTo evaluate the prophylactic efficacy, peptides were intranasally administered to naïve mice and challenged with Afu-allergens/antigens. For evaluation of therapeutic efficacy, the mice were sensitized with Afu-allergens/antigens followed by intranasal administration of peptides. The groups were compared for the levels of Afu-specific antibodies in sera and splenic cytokines evaluated by ELISA. Eosinophil peroxidase activity was examined in the lung cell suspensions and lung inflammation was assessed by histopathogy.ResultsPeptides P1-, P2- and P3 decreased Afu-specific IgE (84.5~98.9%) and IgG antibodies (45.7~71.6%) in comparison with Afu-sensitized mice prophylactically. P1- and P2-treated ABPA mice showed decline in Afu-specific IgE (76.4~88%) and IgG antibodies (15~54%). Increased IgG2a/IgG1 and IFN-γ/IL-4 ratios were observed. P1-P3 prophylactically and P1 therapeutically decreased IL-5 levels and eosinophil peroxidase activity. P1 decreased inflammatory cells' infiltration in lung tissue comparable to non-challenged control.ConclusionAsp f1-derived peptide P1, prophylactically and therapeutically administered to Balb/c mice, is effective in regulating allergic response to allergens/antigens of Afu, and may be explored for immunotherapy of allergic aspergillosis in humans.

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Section: Discussionsupporting

confidence: 89%

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

et al. 2009

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“…Novel animal tests are necessary to elucidate if HFMD and EI may be also pollen-induced delayed-type hypersensitivity diseases in which patients are first sensitized with pollens and then resensitized with continuous pollen exposure during the following years, leading to the onset of these diseases. It is also necessary to study and demonstrate blastoid transformation of lymphocytes sensitized with specific antigenic constituents of pollens, such as Cryj1 and Cryj2 [21], by conducting lymphocyte stimulation tests [22,23] in KD patients.…”

Section: Discussionmentioning

confidence: 99%

A Combination of Cross Correlation and Trend Analyses Reveals that Kawasaki Disease is a Pollen-Induced Delayed-Type Hyper-Sensitivity Disease

Awaya

Nishimura²

2014

IJERPH

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Based on ecological analyses we proposed in 2003 the relation of Kawasaki Disease (KD) onset causing acute febrile systemic vasculitis, and pollen exposure. This study was aimed at investigating the correlation between pollen release and the change in the numbers of KD patients from 1991 to 2002 in Kanagawa, Japan. Short-term changes in the number of KD patients and medium- to long-term trends were analyzed separately. Short-term changes in the number of KD patients showed a significant positive cross correlation (CC) with 9- to 10-month delay following pollen releases, and a smaller but significant CC with 3- to 4-month delay. Further, a temporal relationship revealed by positive CC distribution showed that pollen release preceded KD development, suggesting that pollen release leads to KD development. A trend in patient numbers was fitted by an exponential curve with the time constant of 0.005494. We hypothesized that the trend was caused by the cumulative effects of pollen exposure for elapsed months on patients who may develop KD. By comparing the time constants of fitted exponential curve for each pollen accumulation period with 0.005494, the exposure period was estimated to be 21.4 months, which explains why approximately 50% of patients developed KD within 24 months from birth.

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“…This model of allergic rhinitis produces sneezing, eosinophil infiltration into the nasal mucosa, and Cry j 1-specific IgE production, and should prove useful in studying human cedar pollinosis. We also examined the efficacy of an immunotherapeutic peptide in this model (7,17). However, this model and others only mimic short-term rhinitis and not seasonal rhinitis.…”

Section: Discussionmentioning

confidence: 99%

A new murine model of allergic rhinitis by repeated intranasal Cry j 1 challenge

et al. 2008

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To evaluate the long-lasting effects of new therapeutic approaches to allergies, we established a new model of allergic rhinitis by repeated challenges with intranasal Cry j 1, a common Japanese cedar (Cryptomeria japonica) pollen allergen, in B10.S mice. We sensitized B10.S mice subcutaneously with Cry j 1/alum three times at 1-week intervals. Five weeks after the final sensitization, we challenged the mice by instilling Cry j 1 intranasally for 5 consecutive days starting 1 day after intranasal histamine pretreatment (challenge-1). We challenged the mice by instilling histamine and Cry j 1 intranasally again 12 weeks later (challenge-2). There were significantly more sneezes after challenge-2 than challenge-1. Cry j 1-specific IgE levels in serum were significantly increased in both challenge-1 and 2 after continuous nasal antigen challenge. Serum levels of antiCry j 1 IgE in challenge-2 was 2.3 times higher than after challenge-1. Thus, we have established a new model of seasonal allergic rhinitis in B10.S mice by repeated intranasal antigen challenge, and this model may help elucidate mechanisms of allergic rhinitis and the development of new drugs.

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Cry-Consensus Peptide, a Novel Peptide for Immunotherapy of Japanese Cedar Pollinosis, Induces Th1-Predominant Response in Cry j 1-Sensitized B10.S Mice

Cited by 9 publications

References 45 publications

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

A Combination of Cross Correlation and Trend Analyses Reveals that Kawasaki Disease is a Pollen-Induced Delayed-Type Hyper-Sensitivity Disease

A new murine model of allergic rhinitis by repeated intranasal Cry j 1 challenge

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