A new murine model of allergic rhinitis by repeated intranasal Cry j 1 challenge

Tsunematsu, Miwako; Yamaji, Taketo; Kozutsumi, Daisuke; Murakami, Rika; Nagai, Hiroichi; Kino, Kohsuke

doi:10.2220/biomedres.29.119

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…Sera were prepared from blood obtained from the tail vein of mice two weeks after the intranasal antigen challenge for each therapeutic trial term, at which time the expression of antigen-specific IgE levels peak [31]. Total and Cry j 1-specific IgE in the serum was measured using an enzyme-linked immunosorbent assay (ELISA) and alphaLISA (PerkinElmer, Waltham, MA, USA), respectively.…”

Section: Methodsmentioning

confidence: 99%

Oral Immunotherapy for Pollen Allergy Using T-Cell Epitope-Containing Egg White Derived from Genetically Manipulated Chickens

et al. 2012

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Peptide immunotherapy using T-cell epitopes is expected to be an effective treatment for allergic diseases such as Japanese cedar (Cryptomeria japonica; Cj) pollinosis. To develop a treatment for pollen allergy by inducing oral tolerance, we generated genetically manipulated (GM) chickens by retroviral gene transduction, to produce a fusion protein of chicken egg white lysozyme and a peptide derived from seven dominant human T-cell epitopes of Japanese cedar pollen allergens (cLys-7crp). The transgene sequence was detected in all chickens transduced with the retroviral vector. Transduction efficiency in blood cells correlated to transgene expression. Western blot analysis revealed that cLys-7crp was expressed in the egg white of GM hens. Mice induced to develop allergic rhinitis by Cj pollinosis were fed with cLys-7crp-containing egg white produced by GM chickens. Total and Cj allergen (Cry j 1)-specific IgE levels were significantly decreased in allergic mice fed with cLys-7crp-containing egg white compared with allergic mice fed with normal egg white. These results suggest that oral administration of T-cell epitope-containing egg white derived from GM chickens is effective for the induction of immune tolerance as an allergy therapy.

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Section: Methodsmentioning

confidence: 99%

Oral Immunotherapy for Pollen Allergy Using T-Cell Epitope-Containing Egg White Derived from Genetically Manipulated Chickens

et al. 2012

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“…We generated a novel JCP model by modifying previously reported rhinitis models [26][27][28][29][30]. The use of natural pollen grains, adjuvant-free immunization, and local sensitization via the nasal mucosa provides a more natural exposure to pollen, which resembles cedar pollen sensitization in humans.…”

Section: Discussionmentioning

confidence: 99%

Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets

Zhang

Ohno

Kang

et al. 2014

Vaccine

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“…Optical density at 450 nm was measured by microplate reader (BioTek Instruments, Winooski, VT, USA). Serum Cry j 1-specific IgE was measured according to the previous report (23). Briefly, plates were coated with anti-mouse IgE, washed, and blocked.…”

Section: Measurement Of Total Ige and Cry J 1-specific Igementioning

confidence: 99%

Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology

et al. 2015

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Abstract. Peptide immunotherapy is an attractive approach to relieve allergic symptoms such as rhinitis and asthma. Treatment of Japanse cedar pollinosis (Cryptomeria japonica; Cj), from which over one quarter of Japanese population suffer, is becoming a great concern. Recently, oral feeding of a peptide (7crp) consisting of seven immunodominant human T cell epitopes derived from two enzymes present in Cj pollen was demonstrated to have a benefit in treating Cj pollinosis. In this work, we aimed to apply a novel transcutaneous administration system as a simple and easy peptide delivery for an immunotherapy using a T cell epitope peptide. A modified 7crp peptide (7crpR) which contained triarginine linkers between each epitopes was designed to increase water solubility and was encapsulated in a unique solid-in-oil (S/O) nanodispersion. The S/O nanodispersion consists of a nano-sized peptide-surfactant complex dispersed in an oil vehicle. The S/O nanopartilces having an average diameter of 230 nm facilitated the permeation of the peptide 7crpR into the skin and suppressed serum total IgE and antigen-specific IgE levels in a Cj pollinosis mouse model. Transcutaneous administration of the T cell epitope peptide using the S/O nanodispersion system has potential for future simple and easy immunotherapy of Cj pollinosis.

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A new murine model of allergic rhinitis by repeated intranasal Cry j 1 challenge

Cited by 10 publications

References 17 publications

Oral Immunotherapy for Pollen Allergy Using T-Cell Epitope-Containing Egg White Derived from Genetically Manipulated Chickens

Oral Immunotherapy for Pollen Allergy Using T-Cell Epitope-Containing Egg White Derived from Genetically Manipulated Chickens

Repeated antigen painting and sublingual immunotherapy in mice convert sublingual dendritic cell subsets

Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology

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