Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

Chaudhary, Neelkamal; Mahajan, Lakshna; Madan, Taruna; Kumar, Anil; Raghava, G. P. S.; Katti, S. B.; Haq, W.; Sarma, P. Usha

doi:10.4110/in.2009.9.5.179

Cited by 4 publications

(5 citation statements)

References 53 publications

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“…Thus, they establish the use of allergen peptides to control allergenic reactions in mice and open the way for human study. 27 Our analysis also predicted the same B cell and T cell (MHC-II class) epitopic peptides that are used by Chaudhary et al and suggested a strong correlation between in silico prediction and experimental evidences. We further analyze the epitopic data to screen common epitopic peptides for mouse and human so that they can be tested first on mouse model of A. fumigatus -induced allergy or infection model, and then the promising results from these studies can go for clinical trials for human use.…”

Section: Resultssupporting

confidence: 67%

“…A. fumigatus not only causes ABPA but also is responsible for allergic Aspergillus sinusitis, hypersensitivity pneumonitis, and IgE-mediated asthma. 27 Various strategies have been used to treat allergies such as allergen avoidance and elimination, subcutaneous injection of allergenic extract, and allergen shots. 28 Immunotherapy involves the subcutaneous administration of gradually increasing quantities of allergens or allergen epitopic peptides until a dose has been reached that is effective enough to induce immunologic tolerance to these allergens.…”

Section: Resultsmentioning

confidence: 99%

“…7,44 Furthermore, allergen SIT or allergen shots balance the immune response, specially T H 1 and T H 2 immune response, and control the undesirable immune reactions. 27,45 …”

Section: Resultsmentioning

confidence: 99%

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In silico Identification of Potential Peptides or Allergen Shot Candidates Against Aspergillus fumigatus

Thakur

Shankar

2016

BioResearch Open Access

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Aspergillus fumigatus is capable of causing invasive aspergillosis or acute bronchopulmonary aspergillosis, and the current situation is alarming. There are no vaccine or allergen shots available for Aspergillus-induced allergies. Thus, a novel approach in designing of an effective vaccine or allergen shot candidate against A. fumigatus is needed. Using immunoinformatics approaches from the characterized A. fumigatus allergens, we have mapped epitopic regions to predict potential peptides that elicit both Aspergillus-specific T cells and B cell immune response. Experimentally derived immunodominant allergens were retrieved from . A total of 23 allergenic proteins of A. fumigatus were retrieved. Out of 23 allergenic proteins, 13 of them showed high sequence similarity to both human and mouse counterparts and thus were eliminated from analysis due to possible cross-reactivity. Remaining allergens were subjected to T cell (major histocompatibility complex class I and II alleles) and B cell epitope prediction using immune epitope database analysis resource. Only five allergens have shown a common B and T cell epitopic region between human and mouse. They are Asp f1 {147–156 region (RVIYTYPNKV); Mitogillin}, Asp f2 {5–19 region (LRLAVLLPLAAPLVA); Hypothetical protein}, Asp f5 {305–322 region (LNNYRPSSSSLSFKY); Metalloprotease}, Asp f17 {98–106 region (AANAGGTVY); Hypothetical protein}, and Asp f34 {74–82 region (YIQDGSLYL); PhiA cell wall protein}. The epitopic region from these five allergenic proteins showed potential for development of single peptide- or multipeptide-based vaccine or allergen shots for experimental prioritization.

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Section: Resultssupporting

confidence: 67%

Section: Resultsmentioning

confidence: 99%

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In silico Identification of Potential Peptides or Allergen Shot Candidates Against Aspergillus fumigatus

Thakur

Shankar

2016

BioResearch Open Access

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“…Asp F1 is a ribotoxin and considered the main allergen of A. fumigatus . It was shown that the Asp F1 P1 peptide was able to regulate the allergic response in animals challenged with A. fumigatus allergens and antigens ( Chaudhary et al., 2009 ).…”

Section: Discussionmentioning

confidence: 99%

Pre-Exposure With Extracellular Vesicles From Aspergillus fumigatus Attenuates Inflammatory Response and Enhances Fungal Clearance in a Murine Model Pulmonary Aspergillosis

Souza

Gurgel

Malacco

et al. 2022

Front. Cell. Infect. Microbiol.

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Aspergillus fumigatus is a ubiquitous and saprophytic filamentous fungus and the main etiologic agent of aspergillosis. Infections caused by A. fumigatus culminate in a strong inflammatory response that can evolve into respiratory failure and may be lethal in immunocompromised individuals. In the last decades, it has been demonstrated that extracellular vesicles (EVs) elicit a notable biological response in immune cells. EVs carry a variety of biomolecules, therefore are considered potential antigen delivery vehicles. The role of EVs as a strategy for modulating an effective response against infections caused by A. fumigatus remains unexplored. Here we investigate the use of EVs derived from A. fumigatus as an immunization tool to induce a more robust immune response to A. fumigatus pulmonary infection. In order to investigate that, male C57BL/6 mice were immunized with two doses of EVs and infected with A. fumigatus. Pre-exposure of mice to EVs was able to induce the production of specific IgG serum for fungal antigens. Besides that, the immunization with EVs reduced the neutrophilic infiltrate into the alveoli, as well as the extravasation of total proteins and the production of proinflammatory mediators IL-1β, IL-6, and CXCL-1. In addition, immunization prevented extensive lung tissue damage and also improved phagocytosis and fungus clearance. Noteworthy, immunization with EVs, associated with subclinical doses of Amphotericin B (AmB) treatment, rescued 50% of mice infected with A. fumigatus from lethal fungal pneumonia. Therefore, the present study shows a new role for A. fumigatus EVs as host inflammatory response modulators, suggesting their use as immunizing agents.

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“…Hence, these five proteins with high affinity binding to MHC class I or II epitopes could be used to characterize constituent peptides and develop vaccine candidates for invasive Aspergillus infections or therapeutics for allergy immunotherapy for chronic allergic bronchopulmonary aspergillosis. The promise of this approach is supported by prior work demonstrating that peptides from the protein Asp f1 stimulate the production of Th1 cytokines [60]. However, these proteins have not yet been validated as effective vaccine components.…”

Section: Aspergillosismentioning

confidence: 99%

Advances in Fungal Peptide Vaccines

Silva

Taborda

Nosanchuk

2020

JoF

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Vaccination is one of the greatest public health achievements in the past century, protecting and improving the quality of life of the population worldwide. However, a safe and effective vaccine for therapeutic or prophylactic treatment of fungal infections is not yet available. The lack of a vaccine for fungi is a problem of increasing importance as the incidence of diverse species, including Paracoccidioides, Aspergillus, Candida, Sporothrix, and Coccidioides, has increased in recent decades and new drug-resistant pathogenic fungi are emerging. In fact, our antifungal armamentarium too frequently fails to effectively control or cure mycoses, leading to high rates of mortality and morbidity. With this in mind, many groups are working towards identifying effective and safe vaccines for fungal pathogens, with a particular focus of generating vaccines that will work in individuals with compromised immunity who bear the major burden of infections from these microbes. In this review, we detail advances in the development of vaccines for pathogenic fungi, and highlight new methodologies using immunoproteomic techniques and bioinformatic tools that have led to new vaccine formulations, like peptide-based vaccines.

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Prophylactic and Therapeutic Potential of Asp f1 Epitopes in Naïve and Sensitized BALB/c Mice

Cited by 4 publications

References 53 publications

In silico Identification of Potential Peptides or Allergen Shot Candidates Against Aspergillus fumigatus

In silico Identification of Potential Peptides or Allergen Shot Candidates Against Aspergillus fumigatus

Pre-Exposure With Extracellular Vesicles From Aspergillus fumigatus Attenuates Inflammatory Response and Enhances Fungal Clearance in a Murine Model Pulmonary Aspergillosis

Advances in Fungal Peptide Vaccines

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