Crosslinker-free silk/decellularized extracellular matrix porous bioink for 3D bioprinting-based cartilage tissue engineering

Zhang, Xiao; Liu, Yang; Luo, Chunyang; Zhai, Chenjun; Li, Zuxi; Zhang, Yi; Yuan, Tao; Dong, Shi-Lei; Zhang, Jiyong; Fan, Weimin

doi:10.1016/j.msec.2020.111388

Cited by 108 publications

(94 citation statements)

References 62 publications

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“…Zhang et al developed a crosslinker-free bioink by combining a cartilage extracellular matrix with silk fibroin (SF-dECM bioinks) to promote chondrogenic differentiation in a cartilage-specific microenvironment. The mechanical strength and shape tenability of the bioink were enhanced; this strategy can be regarded as a promising approach for cartilage regeneration [123]. While proliferation and chondrogenic differentiation of mesenchymal stem cells were promoted, their mechanical properties were not comparable to those of native cartilage tissue.…”

Section: Cartilagementioning

confidence: 99%

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Park

Gao

Cho

2021

IJMS

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The musculoskeletal system is a vital body system that protects internal organs, supports locomotion, and maintains homeostatic function. Unfortunately, musculoskeletal disorders are the leading cause of disability worldwide. Although implant surgeries using autografts, allografts, and xenografts have been conducted, several adverse effects, including donor site morbidity and immunoreaction, exist. To overcome these limitations, various biomedical engineering approaches have been proposed based on an understanding of the complexity of human musculoskeletal tissue. In this review, the leading edge of musculoskeletal tissue engineering using 3D bioprinting technology and musculoskeletal tissue-derived decellularized extracellular matrix bioink is described. In particular, studies on in vivo regeneration and in vitro modeling of musculoskeletal tissue have been focused on. Lastly, the current breakthroughs, limitations, and future perspectives are described.

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Section: Cartilagementioning

confidence: 99%

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Park

Gao

Cho

2021

IJMS

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“…Physical crosslinking is a way to produce network of polymer chains by physical treatments like heating, cooling, freeze-drying, or ultrasonication. In this way, the polymer networks are connected by reversible bonds such as ionic interaction, hydrogen bonds, or crystallization ( Zhang et al, 2021 ). The advantage of physical crosslinking methods is that each component does not produce chemical reaction during crosslinking.…”

Section: Cartilage Tissue Engineering For Gp Injuriesmentioning

confidence: 99%

Enlightenment of Growth Plate Regeneration Based on Cartilage Repair Theory: A Review

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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The growth plate (GP) is a cartilaginous region situated between the epiphysis and metaphysis at the end of the immature long bone, which is susceptible to mechanical damage because of its vulnerable structure. Due to the limited regeneration ability of the GP, current clinical treatment strategies (e.g., bone bridge resection and fat engraftment) always result in bone bridge formation, which will cause length discrepancy and angular deformity, thus making satisfactory outcomes difficult to achieve. The introduction of cartilage repair theory and cartilage tissue engineering technology may encourage novel therapeutic approaches for GP repair using tissue engineered GPs, including biocompatible scaffolds incorporated with appropriate seed cells and growth factors. In this review, we summarize the physiological structure of GPs, the pathological process, and repair phases of GP injuries, placing greater emphasis on advanced tissue engineering strategies for GP repair. Furthermore, we also propose that three-dimensional printing technology will play a significant role in this field in the future given its advantage of bionic replication of complex structures. We predict that tissue engineering strategies will offer a significant alternative to the management of GP injuries.

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“…The main advantage of using dECM as a biomaterial is the mimicking of the structure and biological cues of the native tissue that allows for the induction of growth and differentiation of the cellular contingent. For cartilage TE, dECM is already used as a bioink to produce 3D printed cartilaginous substitutes [ 106 , 107 , 108 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…Due to their potential, BM-MSCs could engineer different layers of native cartilage in vitro. MSCs are usually embedded in a hydrogel to reproduce the hyaline-like cartilaginous matrix, due to their excellent hydration properties, such as alginate [ 162 ] GelMA [ 163 ] or dECM-based bioinks [ 106 ]. An important aspect of tissue mimetism is the fiber organization within the different layers; the bioextrusion process can print layers with different alignments, making it possible to reproduce the collagen fibers’ natural organization within the cartilaginous ECM [ 103 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…Very few studies used low cell densities, but they achieved good chondrogenic results [ 154 , 158 ]. For BM-MSCs, the range of densities is comparable to that of chondrocytes, starting from 4 × 10 6 cells/mL [ 175 ] and increasing to as high as 20 × 10 6 cells/mL [ 163 ]; most of the studies were between those two values, thus achieving the best chondrogenic induction possible [ 106 , 162 , 165 , 166 ]. Recently, some research aimed to compare two very low cell densities, 1 and 2 × 10 6 cells/mL, to assess better options for obtaining good chondrogenesis, and they showed that the lowest density seemed to be optimal [ 60 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

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Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Messaoudi

Henrionnet

Bourge

et al. 2020

Cells

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Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

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Crosslinker-free silk/decellularized extracellular matrix porous bioink for 3D bioprinting-based cartilage tissue engineering

Cited by 108 publications

References 62 publications

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Enlightenment of Growth Plate Regeneration Based on Cartilage Repair Theory: A Review

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

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