Cross effects of heat stress and three insecticides on the survival of the codling moth Cydia pomonella (L.): Investigating the molecular and biochemical mechanisms

Perrin, Marie; Moiroux, Joffrey; Maugin, Sandrine; Olivares, Jérôme; Rault, Magali; Siegwart, Myriam

doi:10.1016/j.pestbp.2022.105139

Cited by 7 publications

(4 citation statements)

References 64 publications

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“…7,36,37 Pests have developed multiple complex regulatory mechanisms to enhance their activity of detoxification enzymes to address persecution caused by insecticide exposure. 38,39 In this study, we characterized the transcription factor CpAhR and confirmed that it can effectively activate the expression of key resistance detoxifying genes belonging to GST and P450 superfamilies as well as the enzyme activity of GST but not the P450, thereby increasing the resistance to lambda-cyhalothrin in C. pomonella. Although evolution will gradually differentiate the homologous genes in different species, the transcription factor AhR remains relatively conserved in performing specific functions.…”

Section: ■ Discussionmentioning

confidence: 59%

“…pomonella is a destructive pest of pome fruits worldwide, and the use of chemical insecticides has been considered the most effective and direct control measure against this pest. ,, It has been demonstrated that the overuse of insecticides inevitably leads to the challenging problems of insecticide resistance, which causes almost all pests to be uncontrollable again, including C. pomonella. ,, Pests have developed multiple complex regulatory mechanisms to enhance their activity of detoxification enzymes to address persecution caused by insecticide exposure. , In this study, we characterized the transcription factor CpAhR and confirmed that it can effectively activate the expression of key resistance detoxifying genes belonging to GST and P450 superfamilies as well as the enzyme activity of GST but not the P450, thereby increasing the resistance to lambda -cyhalothrin in C. pomonella.…”

Section: Discussionmentioning

confidence: 99%

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Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella

Liu

Zhang

et al. 2023

J. Agric. Food Chem.

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Aryl hydrocarbon receptor (AhR) enhances insect resistance to insecticides by regulating the detoxification network. Our previous studies have confirmed that overexpressions of cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs) are involved in lambda-cyhalothrin resistance in Cydia pomonella. Here, we report that CpAhR regulates the expression of GST and P450 genes, thus conferring resistance. Expression patterns indicated that the expression of CpAhR was highly induced by lambda-cyhalothrin exposure and upregulated in a lambda-cyhalothrin-resistant population. RNA interference (RNAi) of CpAhR decreases the expression of key resistance-related genes (CpGSTe3, CpCYP9A121, and CpCYP9A122) and the activity of the GST enzyme, reducing the tolerance to lambda-cyhalothrin. Furthermore, β-naphthoflavone, a novel agonist of AhR, was first proven to be effective in increasing CpAhR expression and larval tolerance to lambda-cyhalothrin. These results demonstrate that CpAhR regulates the expression of key detoxifying genes and GST activity, resulting in the development of resistance to lambda-cyhalothrin in C. pomonella.

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Section: ■ Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella

Liu

Zhang

et al. 2023

J. Agric. Food Chem.

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“…It has been demonstrated that prolonged exposure to insecticides inevitably leads to the problem of an increase in insecticide resistance, which causes almost all pests will be uncontrollable again, including C. pomonella [7,36,37]. Pests have developed a variety of complex regulatory mechanisms to enhance the activity of detoxification metabolic enzymes to solve the problem of persecution caused by toxin exposure [38,39]. In this study, we characterized the transcription factor CpAhR and confirmed that it can effectively activate the expression and enzyme activity of GSTs, but not the P450s, and thus increase the resistance of C. pomonella to lambda -cyhalothrin.…”

Section: Discussionmentioning

confidence: 99%

Transcription factor AhR regulates glutathione S-transferases (GSTs) conferring resistance tolambda-cyhalothrin inCydia pomonella

Hu¹,

Liu²,

Zhang³

et al. 2023

Preprint

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Transcription factor aryl hydrocarbon receptor (AhR) can enhance insect resistance to insecticides by regulating the detoxification metabolic network. Our previous studies have confirmed that overexpression of cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs) are both involved in lambda-cyhalothrin resistance in Cydia pomonella. In this study, we report that AhR regulates GSTs thus conferring lambda-cyhalothrin resistance in C. pomonella. Spatiotemporal expression patterns indicated that AhR gene of C. pomonella (CpAhR) was highly expressed in the Malpighian tubules of larvae. Moreover, the expression of CpAhR was induced by lambda-cyhalothrin exposure and was up-regulated in a lambda-cyhalothrin-resistant population. RNA interference (RNAi) of the expression of CpAhR could effectively decrease the relative expression level of CpGSTe3 and enzyme activity of GSTs, but not P450s, further reducing the tolerance of larvae to lambda-cyhalothrin. Furthermore, β-naphthoflavone (BNF), a novel agonist of AhR, can effectively increase the expression of CpAhR and the activity of the GSTs enzyme, resulting in the enhancement of larvae tolerance to lambda-cyhalothrin. These results demonstrate that lambda-cyhalothrin exposure can effectively activate the expression of CpAhR and increase GSTs enzyme thus leading to the development of resistance to lambda-cyhalothrin, which enriches the theory of insecticide resistance regulation in C. pomonella.

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“…In Russian and foreign literature, there is data on effective way of coddling moth` control without negative effect on human health and environment with the use of granulovirus Cydia pomonella (CpGV), a virus with double-chained DNA from the genus Betabaculovirus of the Baculoviridae family. Granulovirus causes systemic infection in coddling moth`s larvae, including the adipose body, tracheal cells, and malpighian tubules, and usually leads to larvae `s death within 7 days after infection [8,9,10]. In Russia in 2022 two granulosa viruses Madex Twin, SK and Carpovirusin, CS were registered on fruit plantations, the efficacy of these products is up to 92-95% [11].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the apple fruits` quality in orchards with Shin-etsu® MD ST, D.

et al. 2022

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The article presents the results of the industrial field trial with pheromones Shin-Etsu® MD STT, D, conducted in 2019-2022 at JSC «Novomikhailovskoe», Tuapse area, Krasnodar region. Twin tubes were hung at every second tree inside the block in the “rosebud” phenophase before flying of the codling moth’s overwintered generation. To track the flying of the phytophage at the experimental site a monitoring pheromone developed for gardens Shin-Etsu® MD STT, D was hung. It was found that in the trial variant more than 14.5 t/ha of harvest in comparison to the control was saved with standard quality of 98.1-98.9%, with costs reduced by 70%.

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Cross effects of heat stress and three insecticides on the survival of the codling moth Cydia pomonella (L.): Investigating the molecular and biochemical mechanisms

Cited by 7 publications

References 64 publications

Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella

Transcription Factor AhR Regulates Glutathione S-Transferases Conferring Resistance to lambda-Cyhalothrin in Cydia pomonella

Transcription factor AhR regulates glutathione S-transferases (GSTs) conferring resistance tolambda-cyhalothrin inCydia pomonella

Assessment of the apple fruits` quality in orchards with Shin-etsu® MD ST, D.

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