Crop optimization and pre-steps standardization to get a Bipolaris euphorbiae-based bioherbicide

Abstract

Making bioproducts available to the market requires finding appropriate processes for mass production and formulation of biological agents. This study aimed at evaluating the Bipolaris euphorbiae production in a solid medium (fermentation in solid substrate) and in a biphasic system (growth in a liquid medium followed by growth in a solid medium), as well as determining the processes for collecting and drying conidia, under laboratory conditions. The influence of the incubation period and inoculum quantity were also investigated. The conidia were dried by using an oven (30ºC, 35ºC, 40ºC, 45ºC, 50ºC, 55ºC and 60ºC), and laminar flow, continuous air flow and aseptic chamber at room temperature. Dry conidia were obtained by sieving and grinding in a ball mill, hammer mill or grain grinder. The conidia viability and sporulation efficiency were evaluated in the solid medium and in the biphasic system. For growth period, the best sporulation on solid medium was obtained after 10 days of incubation, reaching 8.3 x 10(7) conidia g-1 of substrate. The biphasic system did not increase the B. euphorbiae sporulation (4.5 x 10(7) conidia g-1 of substrate), after 14 days, and the amount of liquid inoculum used in this system was not an important factor for increasing its production. The continuous air flow and laminar flow preserved the conidial viability (94.6% and 99.1%, respectively), while promoting a great moisture loss (62.6% and 54.0%, respectively). All the grinding processes reduced the conidia germination (86.2%, 10.5% and 12%, respectively), while sieving allowed the collecting of powdered conidia with high viability (94.8%).

ANÁLISE E DIMENSIONAMENTO DE ESTRUTURA EM MADEIRA Manilkara spp

“…Aspectos como o alto custo e a atual dificuldade de se encontrar moléculas para desenvolvimento de inseticidas; exigências do mercado de exportação por produtos livres de resíduos e outras demandas fitossanitárias, estimulam ainda mais o mercado na busca de alternativas sustentáveis e ecologicamente viáveis (MACHADO et al, 2013;PARRA, 2014). (REDDY et al, 2008;MASCARIN et al, 2014).…”

Formulation and pathogenicity of a bioherbicide for wild poinsettia control

“…The flasks were inoculated with three mycelial discs of 8mm diameter taken from fungal colonies grown on Pontecorvo minimal medium. The flasks were incubatedunder the same conditions described above.After incubation, the colonized mixture was dried, and the conidia were extracted according to Machado et al (2013).…”

Métodos de controle de plantas daninhas em sistemas orgânicos: breve revisão

“…O uso de organismos vivos como estratégia de controle para as plantas daninhas demonstra interesse por apresentar reduzido impacto ambiental, custo acessível, e resultados semelhantes aos obtidos com herbicidas no sistema convencional. Entretanto, organismos vivos exigem cuidados redobrados, sejam relacionados às condições de cultivo, de conservação, de estocagem, sendo que, cada organismo possui condições ótimas de desenvolvimento, que iram proporcionar maior viabilidade e produção (Machado et al, 2013;Moraes et al, 2014). O uso de fungos, bactérias, vírus e insetos para o controle de plantas daninhas se enquadra dentro da perspectiva de controle biológico, e usualmente está sendo conhecida como bioherbicida (Bittencourt e Franzener, 2013; Galon et al, 2016).…”