Critical steps in electronic volume quantification of gingival crevicular fluid: the potential impact of evaporation, fluid retention, local conditions and repeated measurements

Abstract

The findings of the present study confirm the reliability of triplicate readings, and uniqueness of each device and electronic data and the distinct impact of local environmental conditions on the generation/maintenance of calibration scores for each particular device. Furthermore, they underline time-dependent evaporation and fluid retention as additional technical concerns and once again highlight the importance of methodological standardization of the electronic volume quantification process.

Biochemical markers of bone metabolism during early orthodontic tooth movement with aligners

“…To overcome this limitation, we calibrated the Periotron before each GCF sampling. 21 In conclusion, aligners seem to be capable of inducing the same biological responses described for other appliances, at least in the very early stages of orthodontic treatment.…”

“…The readings from the Periotron were converted to an actual volume (microliters) by reference to the standard curve calibrated with human serum. 21 The GCF absorbed on each paper point was diluted with 250 lL of sterile phosphate-buffered saline (pH 7.4), centrifuged (13,000 g at 48C for 15 minutes), and stored at À808C until analysis. 22 The Periotron was always calibrated before GCF sampling.…”

Cytokine measurements in gingival crevicular fluid and periodontal ligament: Are they correlated?

“…Readings from the Periotron were converted to an actual volume (microliters) by reference to a standard curve calibrated with human serum. 13,14 The PDL of each extracted tooth was taken from all root surfaces and immediately scraped using a 13/14 Gracey curette (Maximus, Contagem, Minas Gerais, Brazil) (Fig 1, C). All tooth extractions and sample collections were performed by 1 professional (D.F.M.…”

Biochemical markers of bone metabolism in gingival crevicular fluid during early orthodontic tooth movement

“…In an attempt to reduce this bias, the Periotron was always calibrated before GCF sampling. 27,28 As a pilot experiment for detecting GCF components after the application of force to the teeth by means of elastic separators, this study included a small sample size, measurements of both tension and compression sides, one sampling per site, and a very simple initial tooth displacement (eg, insertion of an elastic separator). In addition, only two time intervals were evaluated-baseline and 7 days for the control sites, and 24 hours and 7 days for the test sites-because these time points were used in similar studies and provided the most significant changes for other biochemical factors.…”

“…The readings from the Periotron were converted to an actual volume (microliters) by reference to the standard curve calibrated with human serum. 27,28 Biochemical Analysis Each GCF sample was diluted in 100 mL 1% Triton X-100/50 mM Tris-HCl (pH 7.5) (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland) and centrifuged at 12,500 rpm for 5 minutes. This procedure was repeated after the addition of another 100 mL of buffer, resulting in a total volume of 200 mL.…”