CRISPR/Cas9 Tool for MicroRNAs Editing in Cardiac Development, Function, and Disease

Abstract

CRISPR/Cas9 is a powerful gene-editing technology. Extensive scientific data exist that the CRISPR/Cas9 system can target small, non-coding, active RNA molecules including microRNAs [miRNAs]. miRNAs have been recognized as key regulators of different cell biological processes, such as modulation of fibrosis and cardiac hypertrophy, as well as the regulation of cardiomyocytes. Also, it has been demonstrated that miRNAs strongly affect organ evolution and the concentration of miRNAs can involve in the differentiation, development, and function of different organs. In addition, the current findings clearly indicate that miRNAs can select and control their targets based on their own concentrations. CRISPR/Cas9 genome-editing technology is a stronger system for stopping miRNAs than previous methods including antisense inhibitors. CRISPR/Cas9 tools can be used to eliminate small areas of DNA and determine miRNA in cases where similar groups of miRNAs are in the same strand. Herein, besides other emerging strategies we critically summarize the recent investigations linking miRNA-targeted therapeutics and CRISPR/Cas9 system, to precisely clarify and combine different delivery platforms and cell-fate engineering of miRNAs function and miRNA-based therapeutic intervention in cardiac development, function, and disease. Based on our findings from the literature, it appears that the use of the CRISPR/Cas technology provides new perspectives for understanding the molecular mechanism of cardiovascular disease and can be effective in the treatment and control of cardiac development, function, and disease in the future.

Long Non-Coding RNAs (lncRNAs) in Heart Failure: A Comprehensive Review

“…In order to ensure the safety of experimental treatments, it is imperative that both preclinical and clinical trials thoroughly evaluate potential off-target effects and long-term repercussions [ 46 , 93 ]. The rapid progress of technology, shown by the emergence of single-cell sequencing and CRISPR-based genome editing, has the potential to expedite the investigation of lncRNA and the creation of therapeutic interventions in a safer and more specific manner [ 94 ]. These techniques have the capability to offer a more profound understanding of lncRNA functionality and facilitate accurate modulation of lncRNA expression.…”