CRISPR-based rapid and ultra-sensitive diagnostic test for
            <i>Mycobacterium tuberculosis</i>

Ai, Jingwen; Zhou, Xian; Teng, Xu; Yang, Minling; Chen, Yuanyuan; He, Guiqing; Pan, Ningp; Cai, Yuwei; Li, Yongjun; Wang, Xiaorui; Su, Hang; Wang, Ting; Zeng, Weiqi; Zhang, Wenhong

doi:10.1080/22221751.2019.1664939

Cited by 139 publications

(118 citation statements)

References 17 publications

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“…(D) Schematic illustration of CRISPR‐MTB. Reprinted with permission from Taylor & Francis Group 63 . (E) Schematic of CRISPR‐Cas–based particular gene sequences detection.…”

Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

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CRISPR‐Cas system for biomedical diagnostic platforms

Wang

Cui

2020

VIEW

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Clustered short palindrome repeats with regular intervals, abbreviated as CRISPR, and functions as a self‐defense system for prokaryotes, detecting particular pathogenic nucleic acid, interfering with the functions of exoteric DNA, and protecting them against foreign invaders. In recent years, CRISPR has attracted increasing interests in the in vitro diagnostic field because of its inherent allele specificity, which is one of the critical factors for the successful application of this technology in the development of high‐precision treatment and diagnosis. Herein, this review article aims to provide an overview of CRISPR‐CRISPR associated proteins (Cas) based biomedical diagnostics, including the biological mechanism, biomaterials, and applications. This paper first briefly introduces the development history and biological characteristics of the CRISPR‐Cas system, and then summarizes the application status and development trend of the CRISPR‐Cas system in the detection and identification of particular pathogens, specifically displaying a brilliant prospect in the most recent outbreak of novel coronavirus (formerly named 2019‐nCoV). Moreover, its potential diagnostic power in oncogene mutations and single nucleotide variations detecting are assembled. Finally, we discuss challenges and future prospects of CRISPR‐Cas system based diagnostic platforms in biomedicine, hoping to further inspire the development of biomedical diagnostics.

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“…(D) Schematic illustration of CRISPR‐MTB. Reprinted with permission from Taylor & Francis Group 63 . (E) Schematic of CRISPR‐Cas–based particular gene sequences detection.…”

Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

“…Another method was obtained by Ai et al developing a rapid assay basing on CRISPR for identifying tuberculosis 63 . Tuberculosis, with a higher risk of death than HIV globally, is the main cause of death in infections 64,65 .…”

Section: Crispr/cas–based In Vitro Diagnosticsmentioning

confidence: 99%

CRISPR‐Cas system for biomedical diagnostic platforms

Wang

Cui

2020

VIEW

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“…Also, we demonstrated that the CRISPR-Cas12a platform could detect the KPC gene encoded by a K. pneumoniae isolate even in complex samples such as blood, emulating a clinical sample. In this line, the potential of the Cas12a effector to detect targets in patient samples has been recently demonstrated by other authors [21].…”

Section: Discussionmentioning

confidence: 61%

CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease

Curti

Pereyra-Bonnet

Repizo

et al. 2020

Emerging Microbes & Infections

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“…Cas9 is also used in cellular models to study for the treatment and diagnosis of polygenic diseases such as diabetes and autism due to multiplexing properties [24]. CRISPR/Cas biotechnology is now expansively and broadly applied across numerous disciplines, including basic sciences, food/crop development, fuel generation, drug development, human genome engineering and gene editing technology against novel diseases like coronavirus (COVID-19 or SARS-CoV2) [8,14,19,22,25,26].…”

Section: Crispr Applications and Designmentioning

confidence: 99%

“…In 2018, several articles were published on the use of CRISPR in diagnostic tests for early-stage cancer detection or for infectious diseases. Further, it was seen as the most important innovation used to develop a rapid CRISPR-based assay for TB detection [25].…”

Section: The Capacity Of Crispr Diagnosticsmentioning

confidence: 99%

CRISPR/Cas9 gene editing technology and its application to the coronavirus disease (COVID-19), a review.

CHEKANI-AZAR¹,

Mombeni²,

BIRHAN³

2020

jwpr

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Introduction. Clustered-Regularly Interspaced Short Palindromic Repeats (CRISPR), and CRISPR associated (Cas) protein (CRISPR/Cas) structures were first identified in E. coli in 1987 and guard prokaryotic cells from any invading pathogens, harmful events and plasmids by recognizing and cutting foreign nucleic acid sequences that contain short palindromic repeats spacer sequences. Several genome editing approaches have been developed based on these mechanisms; the most recent is known as CRISPR/Cas. Before the CRISPR technique was revealed in 2012, editing the genomes of plants and animals took many years and cost hundreds of thousands of dollars. Thus, CRISPR/Cas has attracted significant interest in the scientific community, especially for disease diagnosis and treatment, as it is quicker, less expensive and more precise than other genome editing approaches. The evidence from gene mutations in specific patients generated using CRISPR/Cas can assist in the prediction of the optimal treatment schedule for individual patients and for innovation purposes in other researches like replication in cell culture of coronaviruses like severe acute respiratory syndrome coronavirus-2 (SARS-CoV2 or COVID-19). However, in numerous situations, the effects of the furthermost significant driver mutations are not yet understood and interpretation of the optimal treatment is impossible. CRISPR/Cas classifications feature highly sensitive and selective tools for the detection of various target genes. When we see the next steps of genomic research, it is obvious that genome-wide association studies are relatively new way to identify the genes involved in human disease. Furthermore, CRISPR/Cas provides a tool to manipulate non-coding regions and will thus accelerate examination of these poorly characterized regions of the genome and play a vital role in the progress of whole genome libraries. Aim. We aimed to review the history of CRISPR/Cas, the mechanisms of CRISPR techniques, its current status as a tool for studying both natural mutations and genomic manipulations, and explore how CRISPR/Cas may improve the treatment of diseases.

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CRISPR-based rapid and ultra-sensitive diagnostic test for Mycobacterium tuberculosis

Cited by 139 publications

References 17 publications

CRISPR‐Cas system for biomedical diagnostic platforms

CRISPR‐Cas system for biomedical diagnostic platforms

CRISPR-based platform for carbapenemases and emerging viruses detection using Cas12a (Cpf1) effector nuclease

CRISPR/Cas9 gene editing technology and its application to the coronavirus disease (COVID-19), a review.

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