Abstract:Introduction. Clustered-Regularly Interspaced Short Palindromic Repeats (CRISPR), and CRISPR associated (Cas) protein (CRISPR/Cas) structures were first identified in E. coli in 1987 and guard prokaryotic cells from any invading pathogens, harmful events and plasmids by recognizing and cutting foreign nucleic acid sequences that contain short palindromic repeats spacer sequences. Several genome editing approaches have been developed based on these mechanisms; the most recent is known as CRISPR/Cas. Before the … Show more
“…Finally, the fraction of undetected asymptomatic must not exceed 45%: we assume availability of an easy, rapid and cheap test with a rate of false negative not exceeding 55%, such as CRISPR [29] .…”
Learning and education are two of the biggest world issues of the current pandemic. Unfortunately, it is seen in this work that, due to the length of the incubation period of Covid-19, full opening of schools in the Fall of 2020 seems to be impractical unless the spread of the virus is completely under control in the surrounding region (e.g. with fewer than 5 active cases every million people).
In order to support the possibility of some in-person learning, we model the diffusion of the epidemic within each single school by a SEAIR model with an external source of infection and a suitable loss function, and then evaluate sustainable opening plans. It turns out that blended models, with almost periodic alternations of in-class and remote teaching days or weeks, are generally (close to) optimal. In a prototypical example, the optimal strategy prescribes a school opening of 90 days out of 200 with the number of Covid-19 cases among the individuals related to the school increasing by about 71%, instead of the about 390% increase that would have been a consequence of full opening. As clinical fraction is low in children, these solutions could lead to very few or no symptomatic cases within the school during the whole school year.
Using the density of active cases as a proxy for the number of pre- and asymptomatic, we get an indication for each country of whether either full opening, or blended opening with frequent testing, or no school opening at all, are advisable.
“…Finally, the fraction of undetected asymptomatic must not exceed 45%: we assume availability of an easy, rapid and cheap test with a rate of false negative not exceeding 55%, such as CRISPR [29] .…”
Learning and education are two of the biggest world issues of the current pandemic. Unfortunately, it is seen in this work that, due to the length of the incubation period of Covid-19, full opening of schools in the Fall of 2020 seems to be impractical unless the spread of the virus is completely under control in the surrounding region (e.g. with fewer than 5 active cases every million people).
In order to support the possibility of some in-person learning, we model the diffusion of the epidemic within each single school by a SEAIR model with an external source of infection and a suitable loss function, and then evaluate sustainable opening plans. It turns out that blended models, with almost periodic alternations of in-class and remote teaching days or weeks, are generally (close to) optimal. In a prototypical example, the optimal strategy prescribes a school opening of 90 days out of 200 with the number of Covid-19 cases among the individuals related to the school increasing by about 71%, instead of the about 390% increase that would have been a consequence of full opening. As clinical fraction is low in children, these solutions could lead to very few or no symptomatic cases within the school during the whole school year.
Using the density of active cases as a proxy for the number of pre- and asymptomatic, we get an indication for each country of whether either full opening, or blended opening with frequent testing, or no school opening at all, are advisable.
“…Another aspect to be explored for detecting COVID-19 is making use of CRISPR-Cas gene editing tool which can create wonders in the field of detection (Chekani-Azar et al 2020 ). Making use of CRISPR-Cas has been reported for use in medicine and diagnostics over the years.…”
Section: Current Diagnostic Tests For Covid-19mentioning
The COVID-19 caused by a novel coronavirus, named Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2) has taken a great toll of life affecting lakhs of people around the globe. It was detected initially in Wuhan, China and has spread rapidly to more than 208 countries to date. A range of molecular and immunoassay-based techniques ranging from central laboratory testing to point-of-care tests is urgently needed for the diagnosis and management of COVID-19 patients. Intensive research is going on for the rapid and highly sensitive detection of COVID 19 using varied approach. Hence, this review will focus on the structure of SARS-CoV-2 and recent progress of different detection tool for the detection of COVID-19. This review will also stimulate academics and researcher to update their current technology. Additionally, we also state about the future revolving around the detection of the novel coronavirus. Lately, the way ahead for better management are also put forward.
“…In a sea of different platforms for the possible treatment of COVID-19, CRISPR-Cas mediated gene editing technology remains promising which can be used to manipulate target gene using guide RNA and Cas protein (cleavage protein) [144][145][146]. CRISPR technology using Cas9 [147], Cas12a [84] and Cas13 [148] protein have shown to be used to knock out genes in coronaviruses. Use of CRISPR-Cas13 is advantageous over CRISPR-Cas9 as thousands of Cas13 target sites have been identified in a number of human infection coronaviruses [148] and can be programmed and easily updates CRISPR RNA sequence in response to the change in viral genome sequence which may be due to response to therapy, thus inhibiting viral escape by evolving resistance [149].…”
Section: Emerging Strategies For Covid-19mentioning
COVID-19 manifests regarding extreme acute respiratory conditions caused by a novel beta coronavirus (SARS-CoV-2) which is reported to be the seventh coronavirus to infect humans. Like other SARS-CoVs it has a large positive-stranded RNA genome. But specific furin site in the spike protein, mutation prone and phylogenetically mess Orf1ab separates SARS-CoV-2 from other RNA viruses. Since, the outbreak (February - March 2020) which originated in China, researchers, scientists, and medical professionals are inspecting all possible facts from every possible aspects including its replication, detection and prevention strategies. This led to the prompt identification of its basic biology, genome characterization, structural based functional information of proteins and strategies to prevent its spread. Due to rapid mutation rate, functional characterization of few proteins is still lagging. This review summarizes the recent updates on basic molecular biology of SARS-CoV-2 and prevention strategies undertaken worldwide to tackle COVID-19. This recent information can be implemented for the development and designing of therapeutics against SARS-CoV-2.
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