The substrate is one of the most important factors in the production of seedlings of fruit species, since they guarantee the best conditions of growth and stability of the seedlings in the field. The aim of this study was to evaluate the production of yellow passion fruit seedlings (Passiflora edulis) with different sources and proportions of organic matter.The experimental design was in randomized blocks, in a 3x4 factorial scheme, with 4 replicates, being three sources of organic matter (soil + sand (control), soil + sand + bovine manure, soil + sand + chicken manure and soil + sand + humus) and four ratios (1:1:0, 1:1:1, 1:1:2, 1:1:3), with 10 seedlings per treatment. Daily evaluations were performed to determine the rate of emergence and percentage of germination, and at 60 days after sowing, plant height, leaf number, root length, H/D ratio, stem diameter, fresh mass and dry leaves, stem, root and total. Thus, the organic substrate containing humus and bovine manure provided the best conditions for the production of yellow passion fruit seedlings in a ratio of 1:1:1 to 1:1:2, respectively