Creatine kinase structural changes induced by substrates

Hornikova, Daniela; Heřman, Petr; Mejsnar, J; Večeř, Jaroslav; Žurmanová, Jitka

doi:10.1016/j.bbapap.2008.10.018

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…Creatine Kinase is found in the skeletal muscles of animals, responsible for maintaining energy homeostasis at the sites of high Adenosine TriPhosphate (ATP) (Dieni and Storey, 2009). The activity of CK found in plasma or serum is used to diagnose muscular damage in many species (Hamburg et al, 1991; Hornikova et al, 2009). When CK is found in the blood plasma, it indicates muscle damage (Sáncheza et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

“…It was noted that CK can either be an M or a B monomer subunit. Muscle creatine kinase (M-CK) which can also be denoted by S-CK is found in the M-line of the H-zone of the sarcomere length (Brancaccio et al, 2007; Hornikova et al, 2009). Therefore, the presence of CK in the blood indicates muscle exertion by adverse conditions and poor sheep welfare during the pre-slaughter period (Tadicha et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

Chulayo

Muchenje

2013

Asian Australas. J. Anim. Sci

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The objective of the current study was to determine the effect of pre-slaughter stress, season and breed on the activity of plasma creatine kinase (CK) and the quality of mutton. One hundred and seventy-three (173) castrated sheep from Dormer (DM), South African Mutton Merino (SAMM), Dorper (DP) and Blackhead Persian (BP) sheep breeds were used in the study. The animals were grouped according to age-groups as follows: Group 1 (6 to 8 months), Group 2 (9 to 12 months) and Group 3 (13 to 16 months). Blood samples were collected during exsanguinations using disposable vacutainer tubes for CK analysis. Representative samples of the Muscularis longissimuss thoracis et. lumborum (LTL) were collected from 84 castrated sheep, of different breeds (28 per breed) 24 h after slaughter. The following physico-chemical characteristics of mutton were determined; meat pH (pH24), color (L*, a* and b*), thawing and cooking losses and Warner Braztler Shear Force (WBSF). The activity of plasma CK was significantly higher (p<0.001) in summer (1,026.3±105.06) and lower in winter (723.3±77.75). There were higher values for L* (33.7±0.94), b* (11.5±0.48) and WBSF (29.5±1.46) in summer season than in winter season; L* (29.4±0.64), b* (10.2±0.33) and WBSF (21.2±0.99). The activity of plasma CK was influenced by the type of breed with Dormer having the highest (p>0.001) levels (1,358.6±191.08) of CK. South African Mutton Merino had higher values for pH24 (5.9±0.06), L* (34.2±0.97), b* (12.2±0.50) and WBSF (26.8±1.51) and Blackhead Persian had higher values (35.5±2.17) for cooking loss (CL%) than the other breeds. Computed Principal Component Analyses (PCA) on the activity of plasma CK and physico-chemical characteristics of mutton revealed no correlations between these variables. However, positive correlations were observed between pH24, L*, a*, b*, CL% and WBSF. Relationships between pre-slaughter stress, CK activity and physico-chemical characteristics of mutton were also observed. It was therefore concluded that although mutton quality and creatine kinase were not related, pre-slaughter stress, season and breed affected the activity of creatine kinase and mutton quality.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

Chulayo

Muchenje

2013

Asian Australas. J. Anim. Sci

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“…B chain is specific for brain tissue and M chain is specific for muscle tissue. CK is present in the blood in small amounts and it exists at high levels in cells with high energy requirements such as skeletal, cardiac and smooth muscles, it is also found in kidneys, brain, neuronal tissues, retinal photo receptor cells, spermatozoa and sensory hair cells of the inner ear (2)(3)(4). Increased CK activity has been reported with some physiological conditions like increased muscle activity and exercise yet some disorders such as myocardial necrosis, acute skeletal muscle atrophy, muscular dystrophy, burns, epilepsy, surgical procedures, streptococcus infections also result in a raised activity of this enzyme (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

Total Creatine Kinase and Its Isoenzymes in Cord Blood of Full Term Newborns

Khan¹,

Rathore²

2017

EJMR

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Creatine kinase (CK) is a key enzyme of energy metabolism, especially in muscle tissue. CK has two polypeptide chains of M and B, and three isomers, CK-BB, CK-MB and CK-MM. In some conditions like acute myocardial infarction and Neuromuscular disorders, increased CK activity is used as a part of diagnosis. CK can also be elevated in absence of neuromuscular diseases or cardiac injury, such as strenuous exercise, intramuscular injections etc.Several reports indicate elevated activity of serum CK in the maternal blood during child birth.

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“…The binding of MM-CK to the M-line is precisely regulated by many factors [14,[18][19][20], and thus a screen of the effects of substrates and pH on the MyBPC1-CK interaction using the GSTpulldown assay was performed to elucidate their physiological relevance. The experiments were carried out by adding each of the four CK substrates, ATP, ADP, Cr or PCr to the binding solutions of the GST-pulldown assay with a physiological concentration of 5 mM [29].…”

Section: The Interaction Between Mybpc1 and Mm-ck Is Cr-and Ph-dependentmentioning

confidence: 99%

“…Among the four CK isoenzymes, the MM-CK (muscle-type CK) specifically binds to the myofibril M-line and is associated with the actin-activated myosin ATPase as an intramyofibrillar ATP regenerator [10,[14][15][16][17]. The binding of MM-CK to the M-line is precisely regulated by pH [14], substrates [18,19] and the oxidative status of the enzyme [20]. In addition to the M-line, MM-CK also exists in the I-band of the sarcomere [21].…”

Section: Introductionmentioning

confidence: 99%

Slow skeletal muscle myosin-binding protein-C (MyBPC1) mediates recruitment of muscle-type creatine kinase (CK) to myosin

Chen

Zhao

et al. 2011

Biochemical Journal

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Muscle contraction requires high energy fluxes, which are supplied by MM-CK (muscle-type creatine kinase) which couples to the myofibril. However, little is known about the detailed molecular mechanisms of how MM-CK participates in and is regulated during muscle contraction. In the present study, MM-CK is found to physically interact with the slow skeletal muscle-type MyBPC1 (myosin-binding protein C1). The interaction between MyBPC1 and MM-CK depended on the creatine concentration in a dose-dependent manner, but not on ATP, ADP or phosphocreatine. The MyBPC1-CK interaction favoured acidic conditions, and the two molecules dissociated at above pH 7.5. Domain-mapping experiments indicated that MM-CK binds to the C-terminal domains of MyBPC1, which is also the binding site of myosin. The functional coupling of myosin, MyBPC1 and MM-CK is further corroborated using an ATPase activity assay in which ATP expenditure accelerates upon the association of the three proteins, and the apparent K(m) value of myosin is therefore reduced. The results of the present study suggest that MyBPC1 acts as an adaptor to connect the ATP consumer (myosin) and the regenerator (MM-CK) for efficient energy metabolism and homoeostasis.

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Creatine kinase structural changes induced by substrates

Cited by 7 publications

References 17 publications

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

The Effects of Pre-slaughter Stress and Season on the Activity of Plasma Creatine Kinase and Mutton Quality from Different Sheep Breeds Slaughtered at a Smallholder Abattoir

Total Creatine Kinase and Its Isoenzymes in Cord Blood of Full Term Newborns

Slow skeletal muscle myosin-binding protein-C (MyBPC1) mediates recruitment of muscle-type creatine kinase (CK) to myosin

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