Creatine kinase activity in the detection of carriers of Duchenne muscular dystrophy: comparison of two methods

Tippett, Patricia; Dennis, N R; Machín, David; Price, Christopher P.; Clayton, Barbara E.

doi:10.1016/0009-8981(82)90244-3

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…The results from 50 samples analysed by three different formu lations of the same method were compared with one kit from another manufacturer. The clinical need for this assay was an attem pt to detect female carriers of muscular dystrophy, where very small elevations in the serum creatine kinase levels are seen (Tippett 1982). The lack of good comparability between the methods means that each laboratory would have to establish its own experience with normal controls and carriers and one assay kit before being able to use the test with any confidence.…”

Section: R Ea G En T Mediummentioning

confidence: 99%

Enzymes as reagents in clinical chemistry

Cp¹

1983

Phil. Trans. R. Soc. Lond. B

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Clinical chemistry is concerned with the measurement of substances in biological matter, predominantly blood, serum or plasma. Significant, though small, changes may take place as a prelude to a life-threatening situation. Therefore analytical techniques in clinical chemistry must be sensitive, specific and rapid. Many features of an enzyme-catalysed reaction are incorporated in the design of diagnostic reagents. The specificity of an enzyme may be employed to measure a substrate, or to remove interferents in another reaction. The measurement of substances that act as cofactors, inhibitors or activators can be achieved by the use of the appropriate enzyme. Finally, the enzyme, as a catalyst, can be used as a label in various immunoassay techniques. Clinical chemistry tests are carried out in a wide variety of environments, from the large laboratory undertaking many hundreds of analyses down to a clinic performing only a few tests. Enzymes are therefore employed in analytical systems based on widely differing presentations. Thus enzymes may be employed in a solution medium, immobilized on a surface of the reaction vessel or in a reagent strip. The requirements imposed on the reagent enzyme may be different in all of these situations.

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Section: R Ea G En T Mediummentioning

confidence: 99%

Enzymes as reagents in clinical chemistry

Cp¹

1983

Phil. Trans. R. Soc. Lond. B

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“…However these prior probabilities may be modified following the determination of serum creatinine kinase (CK) concentration, since this metabolite is known to be distributed differently in DMD carriers and control women. Figure 1 shows the distribution of log CK for 48 control women and 22 DMD carriers investigated by Tippett et a1., 3 together with the corresponding fitted Normal curves. There are several alternative statistical methods of calculating the carrier probability, given an individual CK concentration.…”

Section: Introductionmentioning

confidence: 99%

On the standard error of the probability of a particular diagnosis

Machín

Dennis

Tippett

et al. 1983

Statistics in Medicine

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Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase

et al. 1985

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Carbonic anhydrase III (CAIII) and creatine kinase (CK) were measured in plasma samples from a series of females at-risk as carriers of Duchenne muscular dystrophy and compared with control groups. Both plasma CAIII and CK levels were raised in a proportion of carriers. Although measurement of CAIII and CK was no more successful in identifying carriers than CK alone, CAIII could fulfill a useful confirmatory role, particularly for cases with a marginally elevated CK or where the sample is poorly preserved. The difference between the CK and CAIII results could indicate biochemical heterogeneity in the expression of Duchenne muscular dystrophy.

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Creatine kinase activity in the detection of carriers of Duchenne muscular dystrophy: comparison of two methods

Cited by 4 publications

References 22 publications

Enzymes as reagents in clinical chemistry

Enzymes as reagents in clinical chemistry

On the standard error of the probability of a particular diagnosis

Evaluation of carrier detection of Duchenne muscular dystrophy using carbonic anhydrase III and creatine kinase

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