Covalent DNA-Streptavidin Conjugates as Building Blocks for Novel Biometallic Nanostructures

Niemeyer, Christof M.; Bürger, Wolfgang; Peplies, Jörg

doi:10.1002/(sici)1521-3773(19980904)37:16<2265::aid-anie2265>3.0.co;2-f

Cited by 229 publications

(121 citation statements)

References 5 publications

Supporting

Mentioning

120

Contrasting

Order By: Relevance

“…Alternatively, nanoparticle derivatization can be carried out in organic solution (Pathak et al 2001) prior to the coupling to amino-modified DNA oligomers, or the 5 end phosphate group of DNA can be reacted with EDC and imidazole to primary amino groups (Hermanson 2008) present on the particle surface (Skaff & Emrick 2003). Apart from covalent conjugation chemistry, avidin-biotin has also been used as the noncovalent receptor-ligand system for the binding of DNA to nanoparticles, both with biotin-modified DNA (Levy et al 2005) and biotin-modified nanoparticles (Niemeyer et al 1998). …”

Section: (I) Biotin Avidin and Derivativesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,396

1,092

View full text Add to dashboard Cite

Inorganic colloidal nanoparticles are very small, nanoscale objects with inorganic cores that are dispersed in a solvent. Depending on the material they consist of, nanoparticles can possess a number of different properties such as high electron density and strong optical absorption (e.g. metal particles, in particular Au), photoluminescence in the form of fluorescence (semiconductor quantum dots, e.g. CdSe or CdTe) or phosphorescence (doped oxide materials, e.g. Y 2 O 3 ), or magnetic moment (e.g. iron oxide or cobalt nanoparticles). Prerequisite for every possible application is the proper surface functionalization of such nanoparticles, which determines their interaction with the environment. These interactions ultimately affect the colloidal stability of the particles, and may yield to a controlled assembly or to the delivery of nanoparticles to a target, e.g. by appropriate functional molecules on the particle surface. This work aims to review different strategies of surface modification and functionalization of inorganic colloidal nanoparticles with a special focus on the material systems gold and semiconductor nanoparticles, such as CdSe/ZnS. However, the discussed strategies are often of general nature and apply in the same way to nanoparticles of other materials.

show abstract

Section: (I) Biotin Avidin and Derivativesmentioning

confidence: 99%

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Sperling

Parak

2010

Phil. Trans. R. Soc. A.

1,396

1,092

View full text Add to dashboard Cite

show abstract

“…In cases where biotinylated particles were combined with substoichiometric amounts of streptavidin, the particles formed streptavidin-linked aggregates as has been previously observed for other biotinylated particles. 35 The stabilities of passively and specifically linked streptavidin-nanoparticles conjugates were quite different. After being heated at 70 °C for 1 h, passively adsorbed streptavidin was completely dissociated from the surface of the encapsulated Au nanoparticles, as indicated by a failure to titrate any biotin binding sites in these samples.…”

Section: Conjugation With Streptavidinmentioning

confidence: 99%

Enhanced Stability and Bioconjugation of Photo-Cross-Linked Polystyrene-Shell, Au-Core Nanoparticles

et al. 2007

View full text Add to dashboard Cite

Encapsulating Au nanoparticles within a shell of photo-cross-linked block copolymer surfactant dramatically improves the physical and chemical stability of the nanoparticles, particularly when they are applied as bioconjugates. Photo-cross-linkable block copolymer amphiphiles [polystyreneco-poly(4-vinyl were assembled around Au nanoparticles ranging from 12 nm to 108 nm in diameter. UV irradiation cross-linked the PVBP groups on the polymer to yield particles that withstood extremes of temperature, ionic strength, and chemical etching. Streptavidin was attached to [PS-co-PVBP]-b-PAA coated particles using the same noncovalent and covalent conjugation protocols used to bind biomolecules to divinylbenzene-crosslinked polystyrene microspheres. We expect that these particles will be useful as plasmonic, highly light-scattering and light-absorbing analogs to fluorescently labeled polystyrene nanospheres.

show abstract

“…For each application, NPs of different size and shape are needed (Jin et al 2001;Sun and Xia 2002), thereby synthetic protocols for the production of size and shape controlled monodisperse NPs are required. Since Faraday's pioneering work in 1857 on the synthesis of colloidal gold by reducing NaAuCl 4 with a solution of phosphorus in carbon disulphide ( citrate or sodium borohydride, followed by surface modification of the produced particles with suitable capping ligands and organic solvents (Niemeyer et al 1998;Lévy et al 2004), raised environmental concerns, because of the toxic compounds used in the process. Also, it is difficult to obtain NPs of defined size and shapes (e.g.…”

Section: Introductionmentioning

confidence: 99%

A green chemical approach for the synthesis of gold nanoparticles: characterization and mechanistic aspect

Das

Marsili

2010

Rev Environ Sci Biotechnol

102

View full text Add to dashboard Cite

This IRCSET-EMPOWER (Irish Research Council for Science, Engineering andTechnology Postdoctoral Research Grant) project aims to improve current methodology for the synthesis of metal nanoparticles (NPs). The development of efficient methodology for metal nanomaterials synthesis is an economical and environmental challenge. While the current methods for NPs synthesis are often energy-intensive and involve toxic chemicals, NPs biosynthesis can be carried on at circumneutral pH and mild temperature, resulting in low cost and environmental impact. Nanomaterial biosynthesis has been already observed in magnetotactic bacteria, diatoms, and S-layer bacteria, however, controlled NPs biosynthesis is a relatively new area of research with considerable potential for development. A thorough understanding of the biochemical mechanism involved in NPs biosynthesis is needed, before biosynthetic methods can be economically competitive. The analysis and identification of active species in the nucleation and growth of metal NPs is a daunting task, due to the complexity of the microbial system. This project work focuses on the controlled biosynthesis of gold NPs by fungal microorganisms and aims to determine the biochemical mechanism involved in nucleation and growth of the particles.

show abstract

Covalent DNA-Streptavidin Conjugates as Building Blocks for Novel Biometallic Nanostructures

Cited by 229 publications

References 5 publications

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Surface modification, functionalization and bioconjugation of colloidal inorganic nanoparticles

Enhanced Stability and Bioconjugation of Photo-Cross-Linked Polystyrene-Shell, Au-Core Nanoparticles

A green chemical approach for the synthesis of gold nanoparticles: characterization and mechanistic aspect

Contact Info

Product

Resources

About