Covalent attachment of laccase to carboxymethyl-botryosphaeran in aqueous solution for the construction of a voltammetric biosensor to quantify quercetin

Gomes, Adriana; Mattos, Gabriel Junquetti; Coldibeli, Bruna; Dekker, Robert F. H.; Dekker, Aneli M. Barbosa; Sartori, Elen Romão

doi:10.1016/j.bioelechem.2020.107543

Cited by 17 publications

(11 citation statements)

References 44 publications

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“…The stable covalent coupling led to very little loss in enzyme activity and consequently resulted in an excellent analytical performance with promising stability and selectivity. The electrochemical biosensor exhibited good agreement with HPLC results with a confidence level of 95% . The extensive coupling chemistry and the vast variety of immobilization supports available can be exploited in modulating the enzymatic activity of oxidoreductases upon covalent immobilization.…”

Section: Immobilization Techniquesmentioning

confidence: 66%

“…On the other hand, many researchers have reported the use of neat samples of beverages such as wines, tea, and fruit juices without any pretreatment step. 5,17 The excellent agreement between the values obtained from biosensors and HPLC analysis suggests that the effect of the sample matrix is insignificant during the proposed biosensing analysis. In contrast, for solid samples, the quantitative analysis of antioxidants is normally achieved by preparing and analyzing sample extracts.…”

Section: Oxidoreductases As Bioreceptorsmentioning

confidence: 71%

“…Juices, wines, and tea infusions are routinely diluted with distilled water or buffer before use. − There are varied dilutions that have been performed; for instance, for wines, dilutions as high as 1:100 to as low as 1:5 are stated in the literature. On the other hand, many researchers have reported the use of neat samples of beverages such as wines, tea, and fruit juices without any pretreatment step. , The excellent agreement between the values obtained from biosensors and HPLC analysis suggests that the effect of the sample matrix is insignificant during the proposed biosensing analysis. In contrast, for solid samples, the quantitative analysis of antioxidants is normally achieved by preparing and analyzing sample extracts.…”

Section: Oxidoreductases As Bioreceptorsmentioning

confidence: 91%

“…This suggests the presence of interaction among the polyphenols that can provide a synergistic effect and improve or modify the signal. 8 On the other hand, researchers have constructed B. rhodina laccase-based biosensors that are capable of detecting rutin 5 and quercetin 17 in beverages. The results for the determination of rutin and quercetin in beverages as well as biological samples were in agreement with HPLC quantifications at a confidence level of 95%.…”

Section: Oxidoreductases As Bioreceptorsmentioning

confidence: 99%

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Recent Advances in the Development of Oxidoreductase-Based Biosensors for Detection of Phenolic Antioxidants in Food and Beverages

Lzaod

Dutta

2022

ACS Omega

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Antioxidants are known to exhibit a protective effect against reactive oxygen species (ROS)-related oxidative damage. As a result, inclusion of exogenous antioxidants in the diet has greatly increased. In this sense, detection and quantification of such antioxidants in various food and beverage items are of eminent importance. Monophenols and polyphenols are among the most prominent natural antioxidants. In this regard, biosensors have emerged as a simple, fast, and economical method for determination of such antioxidants. Owing to the fact that majority of the phenolic antioxidants are electroactive, oxidoreductase enzymes are the most extensively availed bioreceptors for their detection. Herein, the different types of oxidoreductases that have been utilized in biosensors for the biorecognition and quantification of natural phenolic compounds commonly present in foods and beverages are discussed. Apart from the most accustomed electrochemical biosensors, this review sheds light on the alternative transduction systems for the detection of phenolic antioxidants. Recent advances in the strategies involved in enzyme immobilization and surface modification of the biosensing platform are analyzed. This review aims to provide a brief overview of the latest developments in biosensor technology for phenolic antioxidant analysis in foodstuffs and future directions in this field.

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Section: Immobilization Techniquesmentioning

confidence: 66%

Section: Oxidoreductases As Bioreceptorsmentioning

confidence: 71%

Section: Oxidoreductases As Bioreceptorsmentioning

confidence: 91%

Section: Oxidoreductases As Bioreceptorsmentioning

confidence: 99%

See 2 more Smart Citations

Recent Advances in the Development of Oxidoreductase-Based Biosensors for Detection of Phenolic Antioxidants in Food and Beverages

Lzaod

Dutta

2022

ACS Omega

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“…Furthermore, another covalent immobilization strategy widely used in many laccasebased biosensors is the EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/Nhydroxysuccinimide) activation reaction [67,68,70,77]. We have schematically drawn the typical EDC/NHS immobilization strategy to inform readers (Figure 11).…”

Section: Covalent-based Laccase Immobilizationmentioning

confidence: 99%

Recent Advances in the Development of Laccase-Based Biosensors via Nano-Immobilization Techniques

et al. 2022

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Monitoring phenolic compounds is critical in the environmental, food, and medical sectors. Among many recent advanced detection platforms, laccase-based biosensing platforms gave very rapid, effective, online, and in situ sensing of phenolic compounds. In laccase-based biosensors, laccase immobilization techniques have a vital role. However, a detailing of the advancements in laccase immobilization techniques employed in laccase-based biosensors is lacking in the literature. Thus, in this review, we assessed how the nano-immobilization techniques shaped the laccase biosensing platforms. We discussed novel developments in laccase immobilization techniques such as entrapment, adsorption, cross-linking, and covalent over new nanocomposites in laccase biosensors. We made a comprehensive assessment based on the current literature for future perspectives of nano-immobilized laccase biosensors. We found the important key areas toward which future laccase biosensor research seems to be heading. These include 1. A focus on the development of multi-layer laccase over electrode surface, 2. The need to utilize more covalent immobilization routes, as they change the laccase specificity toward phenolic compounds, 3. The advancement in polymeric matrices with electroconductive properties, and 4. novel entrapment techniques like biomineralization using laccase molecules. Thus, in this review, we provided a detailed account of immobilization in laccase biosensors and their feasibility in the future for the development of highly specific laccase biosensors in industrial, medicinal, food, and environmental applications.

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Botryosphaeran

Dekker¹,

Barbosa-Dekker²

2021

Polysaccharides of Microbial Origin

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Covalent attachment of laccase to carboxymethyl-botryosphaeran in aqueous solution for the construction of a voltammetric biosensor to quantify quercetin

Cited by 17 publications

References 44 publications

Recent Advances in the Development of Oxidoreductase-Based Biosensors for Detection of Phenolic Antioxidants in Food and Beverages

Recent Advances in the Development of Oxidoreductase-Based Biosensors for Detection of Phenolic Antioxidants in Food and Beverages

Recent Advances in the Development of Laccase-Based Biosensors via Nano-Immobilization Techniques

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