Coupling hybridization chain reaction with catalytic hairpin assembly enables non-enzymatic and sensitive fluorescent detection of microRNA cancer biomarkers

Wei, Yulian; Zhou, Wenjiao; Li, Xin; Chai, Yaqin; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.bios.2015.09.053

Cited by 69 publications

(28 citation statements)

References 41 publications

(36 reference statements)

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“… 42 , 43 miRs have been demonstrated to be associated with the occurrence and development of human cancer, by behaving as tumor suppressors or oncogenes. 44 The association between miR deregulation and cancer has led many scientists to do a lot of research to clarify the role of miRs in various cancers. More and more research results support the idea that aberrant of miR expression level is the rule rather than the exception in cancer development.…”

Section: Discussionmentioning

confidence: 99%

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

Kong

Han

Deng

et al. 2018

OTT

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ObjectiveMicroRNA (miR)-431 plays an essential role in various human cancer types, particularly in the process of invasion. However, the function and mechanism of miR-431-5p in the invasion of hepatocellular carcinoma (HCC) remain undefined.MethodsThe expression levels of miR-431-5p and its potential target protein UROC28 in hepatocellular carcinoma cells and tissues were detected, and the levels of EMT markers in vivo and in vitro were also detected.ResultsMiR-431-5p was downregulated in HCC cell lines and tissues and associated with vascular invasion and tumor encapsulation. Furthermore, miR-431-5p was able to influence the epithelialto-mesenchymal transition (EMT) process in HCCLM3 and HUH7 cells. Mechanistically, it was discovered that miR-431-5p repressed invasion by targeting UROC28. Furthermore, miR-431-5p influenced the EMT markers in HCCLM3 and HUH7 cells by downregulating UROC28 expression. Similarly, in vivo assays confirmed that miR-431-5p upregulation in HCC cells remarkably inhibited tumor proliferation and influenced the EMT markers.ConclusionThe current study has demonstrated that the miR-431-5p/UROC28 axis acts possible influence on the EMT in HCC. Upregulation of miR-431-5p could be an original approach for inhibiting tumor invasion.

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Section: Discussionmentioning

confidence: 99%

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

Kong

Han

Deng

et al. 2018

OTT

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“…Hairpin chain reaction-based in situ mRNA hybridization cdk-2 mRNA FISH was performed using hairpin chain reaction as described previously (Huang et al, 2016;Wei et al, 2016;Xuan and Hsing, 2014), except that the analysis in the present study was conducted on dissected germlines that were fixed in a 3% paraformaldehyde and 0.25% glutaraldehyde solution at room temperature for 2 h. The probes were obtained from Molecular Instruments and the manufacturer's instructions were followed.…”

Section: Western Blot Analysismentioning

confidence: 99%

GSK-3 promotes S-phase entry and progression in C. elegans germline stem cells to maintain tissue output

et al. 2018

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Adult germline stem cells (GSCs) and mouse embryonic stem cells (mESCs) exhibit a non-canonical cell cycle structure with an abbreviated G1 phase and phase-independent expression of Cdk2 and cyclin E. Mechanisms that promote the abbreviated cell cycle remain unknown, as do the consequences of not maintaining an abbreviated cell cycle in these tissues. In GSCs, we discovered that loss of results in reduced GSC proliferation without changes in differentiation or responsiveness to GLP-1/Notch signaling. We find that DPL-1 transcriptional activity inhibits CDK-2 mRNA accumulation in GSCs, which leads to slower S-phase entry and progression. Inhibition of or transgenic expression of CDK-2 via a heterologous germline promoter rescues the S-phase entry and progression defects of the mutants, demonstrating that transcriptional regulation rather than post-translational control of CDK-2 establishes the abbreviated cell cycle structure in GSCs. This highlights an inhibitory cascade wherein GSK-3 inhibits DPL-1 and DPL-1 inhibits transcription. Constitutive GSK-3 activity through this cascade maintains an abbreviated cell cycle structure to permit the efficient proliferation of GSCs necessary for continuous tissue output.

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“…More recently, a cascaded HCR–CHA circuit has been successfully developed for the amplified nucleic acid detection. 43 – 45 …”

Section: Introductionmentioning

confidence: 99%

Construction of an autonomously concatenated hybridization chain reaction for signal amplification and intracellular imaging

et al. 2018

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Coupling hybridization chain reaction with catalytic hairpin assembly enables non-enzymatic and sensitive fluorescent detection of microRNA cancer biomarkers

Cited by 69 publications

References 41 publications

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

GSK-3 promotes S-phase entry and progression in C. elegans germline stem cells to maintain tissue output

Construction of an autonomously concatenated hybridization chain reaction for signal amplification and intracellular imaging

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