miR-431-5p alters the epithelial-to-mesenchymal transition markers by targeting <em>UROC28</em> in hepatoma cells

Abstract

ObjectiveMicroRNA (miR)-431 plays an essential role in various human cancer types, particularly in the process of invasion. However, the function and mechanism of miR-431-5p in the invasion of hepatocellular carcinoma (HCC) remain undefined.MethodsThe expression levels of miR-431-5p and its potential target protein UROC28 in hepatocellular carcinoma cells and tissues were detected, and the levels of EMT markers in vivo and in vitro were also detected.ResultsMiR-431-5p was downregulated in HCC cell lines and tissues and associated with vascular invasion and tumor encapsulation. Furthermore, miR-431-5p was able to influence the epithelialto-mesenchymal transition (EMT) process in HCCLM3 and HUH7 cells. Mechanistically, it was discovered that miR-431-5p repressed invasion by targeting UROC28. Furthermore, miR-431-5p influenced the EMT markers in HCCLM3 and HUH7 cells by downregulating UROC28 expression. Similarly, in vivo assays confirmed that miR-431-5p upregulation in HCC cells remarkably inhibited tumor proliferation and influenced the EMT markers.ConclusionThe current study has demonstrated that the miR-431-5p/UROC28 axis acts possible influence on the EMT in HCC. Upregulation of miR-431-5p could be an original approach for inhibiting tumor invasion.

miR-431-5p regulates cell proliferation and apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis by targeting XIAP

“…Upregulation of miR-431-5p prohibits cell proliferation and invasion via ATG3 in colon cancer [19]. Furthermore, miR-431-5p suppresses invasion by targeting UROC28 in hepatocellular carcinoma [18]. Consistent with previous studies, we observed that miR-431-5p overexpression suppresses cell proliferation and G0/G1-to-S phase transition but promoted apoptosis in RA FLSs, suggesting the potential of miR-431-5p in targeting excessively proliferative FLSs in patients with RA.…”

“…In this study, we have demonstrated that miR-431-5p is downregulated in synovial tissues and FLSs from patients with RA, indicating its potential role in the development of RA. miR-431-5p is associated with the development of various cancers, such as lung cancer, glioblastoma multiforme, colon cancer, and hepatocellular carcinoma [17][18][19]25]. miR-431-5p modulated cell proliferation, apoptosis, autophagy, migration, invasion, and angiogenesis.…”

“…We have shown that miRNAs are important for FLSs proliferation, apoptosis, and inflammation in individuals with RA [15,16]. Studies have recently demonstrated that miR-431-5p is dysregulated in various human cancers, such as lung, liver, colon cancer, and squamous cell carcinoma [17][18][19][20]. However, the role of miR-431-5p in RA remains to be understood.…”

miR-431-5p regulates cell proliferation and apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis by targeting XIAP

“…Upregulation of miR-431-5p prohibits cell proliferation and invasion via ATG3 in colon cancer (18). Furthermore, miR-431-5p suppresses invasion by targeting UROC28 in hepatocellular carcinoma (17). Consistent with previous studies, we observed that miR-431-5p overexpression suppresses cell proliferation and G0/G1-to-S phase transition, but promoted apoptosis in RA FLSs, suggesting the potential of miR-431-5p in targeting excessively proliferative FLSs in patients with RA.…”

miR-431-5p regulates cell proliferation and apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis by targeting XIAP

“…Studies have recently demonstrated that miR-431-5p is dysregulated in various human cancers, such as lung, liver, colon cancer, and squamous cell carcinoma (17)(18)(19)(20). However, the role of miR-431-5p in RA remains to be understood.…”