Construction of an autonomously concatenated hybridization chain reaction for signal amplification and intracellular imaging

Abstract

The concatenated hybridization chain reaction (C-HCR) was constructed as a versatile and robust tool for signal amplification and intracellular imaging, which was attributed to the synergistic amplification effect between HCR-1 and HCR-2.

A DNAzyme-amplified DNA circuit for highly accurate microRNA detection and intracellular imaging

“…51,52 Yet these individual ampliers are always confronted with limited versatilities and unsatisfactory signal gains, which could be solved by their integration with other amplication strategies. [53][54][55] The in-depth integration of these different amplication methods attracts more attention since a higher signal gain is expected to be realized in live cell analysis.…”

Programmable intracellular DNA biocomputing circuits for reliable cell recognitions

“…It provides a general and efficient amplification principle for assembling dsDNA copolymeric nanowires via trigger-initiated successive cross-opening of multiple hairpin substrates. The HCR has been successfully utilized for detecting various nucleic acid analytes44–47 while little attention has been given to HCR-based DNA biocircuits especially in the complex intracellular environment 48–50. The facile design and intrinsic amplification features of the HCR are expected to promote the integration of the HCR into intracellular DNA computing circuits for diagnosis purposes.…”

Single-molecule dynamic DNA junctions for engineering robust molecular switches

“…[7][8][9][10] Strand displacement-based amplication strategies are also frequently adopted for molecular switches. [11][12][13] However, the greatest challenge for a TMSD-based molecular switch is the initiation of the reaction in the absence of an input, known as leakage, contributing to a non-negligible amount of background, which limits the engineering of more specic and sensitive tools. [14][15][16] Efforts have been made to reduce the leakage, such as scalable junction congurations, 17 toeless strategies, 16 and mismatch involved TMSD.…”