Cost structure and estimation for the recycling of salt in a protein extraction process

Greve, Arend; Kula, Maria‐Regina

doi:10.1007/bf00369255

Cited by 37 publications

(17 citation statements)

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“…Due to the high cost of the polymers and difficulty in isolating the extracted molecules from the polymer phase by back extraction, these systems can not be used in a large scale, especially for production of bulk chemicals. Recently, short chain alcohol/salt system has been used as a novel aqueous twophase system to purify natural compounds because of its advantages such as low cost, easy recovery of alcohol by evaporation and simple scale-up [16]. Ethanol can form stable and adjustable aqueous twophase system with inorganic salt solutions, which might be due to salting-out and the low solubility of inorganic salt in ethanol.…”

Section: Introductionmentioning

confidence: 99%

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths using an ethanol/phosphate system

Jiang

Dai

et al. 2009

Process Biochemistry

117

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Section: Introductionmentioning

confidence: 99%

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths using an ethanol/phosphate system

Jiang

Dai

et al. 2009

Process Biochemistry

117

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“…However, recycling higher percentages runs the risk of recycling contaminant species. There have also been studies on the alternative of recycling salt34, 35 that reported the technical and economical feasibility. Most of these flowsheet calculation studies were specific to certain cases and used embedded empirical correlations to represent equilibrium behavior in ATPE 15–20.…”

Section: Introductionmentioning

confidence: 99%

Flowsheet simulation of aqueous two‐phase extraction systems for protein purification

Ahmad

Hauan

Przybycien

2010

J of Chemical Tech & Biotech

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BACKGROUND: Aqueous two-phase extraction (ATPE) has many advantages as an efficient, inexpensive large-scale liquid-liquid extraction technique for protein separation. However, the realization of ATPE as a protein separation technology at industrial scales is rather limited due to the large, multidimensional design space and the paucity of design approaches to predict phase and product behavior in an integrated fashion with overall system performance. This paper describes a framework designed to calculate suitable flowsheets for the extraction of a target protein from a complex protein feed using ATPE. The framework incorporated a routine to set up flowsheets according to target protein partitioning behavior in specific ATPE systems and a calculation of the amounts of phase-forming components needed to extract the target protein. The thermodynamics of phase formation and partitioning were modeled using Flory-Huggins theory and calculated using a Gibbs energy difference minimization approach.

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“…A new type of aqueous two-phase (ATP) extraction method has been recently used for purification of biologically produced biomolecules [18]. ATP extraction has been widely applied in the separation of biomacromolecules, such as proteins and nucleic acids because of its mild conditions and high capacity [18][19][20][21][22]. Up to now, most ATP systems used for purification were based either on PEG/salt systems or polymer/polymer (e.g.…”

Section: Introductionmentioning

confidence: 99%

Selection and optimization of an aqueous two‐phase system for the recovery of 1,3‐propandiol from fermentation broth

Aydoğan¹,

Bayraktar²,

Mehmetoǧlu³

et al. 2010

Engineering in Life Sciences

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In this study a suitable alcohol/salt aqueous two‐phase (ATP) system was selected for the recovery of 1,3‐propandiol (1,3‐PD) from fermentation broth. From the different alcohol/salt systems studied the ethanol and dipotassium hydrogen phosphate ATP system appeared to be favorable. To examine the potential of this ATP system the partition coefficient of 1,3‐PD in synthetic solutions was first optimized with the response surface methodology. The parameters studied were concentrations of ethanol (21.99–38.81% w/w), dipotassium hydrogen phosphate (14.99–31.81% w/w) and 1,3‐PD (6.36–73.64 g/L). The optimum conditions were found to be 35.39% w/w for ethanol, 28.40% w/w for dipotassium hydrogen phosphate and 73.6 g/L for 1,3‐PD. Under these conditions the maximum partition coefficient of 1,3‐PD and the extraction yield were determined as 23.14 and 97.82%, respectively. The optimum extraction conditions were then used to guide the recovery of 1,3‐PD from a real fermentation broth. The partition coefficient and extraction yield of 1,3‐PD reached 20.28–97.20% in this case, respectively. A favorable partition of the organic acids lactate, acetate and butyrate in the bottom phase was also achieved. We have also studied the removal of cells and macromolecules from the broth. Removal ratio of cells and proteins were 96.47 and 93.05%, respectively. Thus, the ethanol/dipotassium hydrogen phosphate ATP system appears to be an interesting alternative or can be used as one useful step in the downstream processing of 1,3‐PD from fermentation broth.

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Cost structure and estimation for the recycling of salt in a protein extraction process

Cited by 37 publications

References 1 publication

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths using an ethanol/phosphate system

Aqueous two-phase extraction of 2,3-butanediol from fermentation broths using an ethanol/phosphate system

Flowsheet simulation of aqueous two‐phase extraction systems for protein purification

Selection and optimization of an aqueous two‐phase system for the recovery of 1,3‐propandiol from fermentation broth

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