Aqueous two‐phase extraction systems were developed to separate intracellular proteins from cell debris. To economize on the use of chemicals as well as to minimize environmental pollution, a closed mode of operation was sought. Various approaches to achieve recycling of salt from the primary lower phase containing 30–60% (w/w) of the added salt together with cell debris, proteins and nucleic acids were studied. Techniques assessed included microfiltration, heat treatment and the extraction with phase systems formed by aliphatic alcohols. With 20% (w/w) primary lower phase it was found to be possible to separate 95% of the salt in 3–4 theoretical steps using counter‐current extraction.
Im Rahmen eines Projektes soll eine Salpetersaure-Kolonne fur eine Glyoxalsaure-Anlage optimiert werden. In der Kolonne werden die NO,-haltigen Abgase durch Absorption in Wasser gereinigt. Die dabei anfallende Salpetersaure wird wieder in den ProzeB zuriickgefahren. Aufbauend auf der umfangreichen Literatur zu dieser Fragestellung sollte ein Modell der Reaktionskolonne erstellt werden. Die dazu notwendigen Ansatze fur die Kinetiken und Stoffubergangsberechnungen sollen vollstandig der Literatur entstammen, das heist, da8 keine Anpassungen durchgefuhrt werden sollen. Das Modell sol1 anhand einer Gegenuberstellung der berechneten Konzentrationen mit dem vermessenen Konzentrationsprofil der Salpetersaure uber die Kolonne uberpruft werden.
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