Cost Efficiency of Cryopreservation as a Long‐Term Conservation Method for Coffee Genetic Resources

Dulloo, M. E.; Ebert, Andreas W.; Dussert, Stéphane; Gotor, Elisabetta; Astorga, Carlos; Vásquez, Nelly; Rakotomalala, Jean-Jacques; Rabemiafara, A.; Eira, M. T. S.; Bellachew, B.; Omondi, Chrispine O.; Engelmann, Florent; Anthony, François; Watts, Jamie; Qamar, Zahida; Snook, L.K.

doi:10.2135/cropsci2008.12.0736

Cited by 43 publications

(26 citation statements)

References 24 publications

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“…Safety, the need for little space and the lowest cost, as well as other advantages offered by the cryopreservation of genetic resources of coffee in relation to other methods of ex situ conservation (literally means, "off-site conservation") (Dulloo et al, 2009) has promoted research and advances of this technique for the preservation of this species. However, many questions about the behavior of these intermediate seeds, partially tolerant to desiccation and low temperatures (Ellis et al, 1990) are still researched by the scientific community in order to elucidate the obstacles that hamper their cryopreservation success.…”

Section: Introductionmentioning

confidence: 99%

Exploratory studies for cryopreservation of Coffea arabica L. seeds

et al. 2017

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-Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests.Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.Index terms: silica gel, water content, slow cooling, liquid nitrogen, coffee.Estudos exploratórios para a criopreservação de sementes de Coffea arabica L.RESUMO -A criopreservação é uma opção viável para a conservação de germoplasma de café. Entretanto, para que essa técnica apresente total sucesso é de fundamental importância realizar pesquisas que garantam a manutenção da integridade celular antes e após a imersão em NL. Portanto, o objetivo neste estudo foi investigar o teor de água, velocidade e temperatura final de resfriamento mais adequados para criopreservar sementes de Coffea arabica L. As sementes foram secadas em sílica gel até teores de água de 5, 10, 15, 20, 30 e 40% bu, submetidas a tratamentos de resfriamento lento nas velocidades -1, -3 e -5 ºC min -1 até às temperaturas finais de -40, -50 e -60 ºC e diretamente imersas em NL. Após armazenamento as sementes foram reaquecidas por dois minutos à 40 ºC. A sobrevivência e a viabilidade de sementes e embriões foram avaliadas por meio do teste de tetrazólio e de germinação. Por meio do teste de tetrazólio, observa-se que embriões excisados das sementes criopreservadas são menos sensíveis à criopreservação quando comparados às sementes inteiras. O teor de água de 20% bu permite maior sobrevivência das sementes de Coffea arabica L., bem como de embriões zigóticos após a criopreservação, dependendo das velocidades e temperaturas finais de pré-resfriamento.Termos para indexação: sílica gel, teor de água, resfriamento lento, nitrogênio líquido, café.

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Section: Introductionmentioning

confidence: 99%

Exploratory studies for cryopreservation of Coffea arabica L. seeds

et al. 2017

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“…As cost factors determine feasibility and power of any germplasm preservation, various analyses and assessments were already published (Dulloo et al 2009;Garming et al 2009;IPGRI/CIAT 1994;Koo et al 2004;Li and Pritchard 2009). Cryopreservation was also subject of preliminary economical considerations in Germany and France (Harvengt et al 2004;Keller 2006;Schäfer-Menuhr et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Comparing costs for different conservation strategies of garlic (Allium sativum L.) germplasm in genebanks

Keller

Zanke

Senula

et al. 2012

Genet Resour Crop Evol

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The maintenance of plant genetic resources in living plant collections (genebanks) causes costs due to employment of staff, usage of buildings, equipment and consumables. Since this is especially challenging in vegetatively propagated material, studies were performed for the case of garlic, which is one of the major vegetatively maintained crops in the genebank of IPK Gatersleben. Data were recorded to compare various scenarios of the main strategies field maintenance and cryopreservation. A spreadsheet tool was developed to be used for cost assessment and for drawing conclusions concerning the most effective way of maintenance. Field culture is cheaper in the short term, whereas after a break-even point cryopreservation becomes the more efficient storage method in the long term. This breakeven point depends on the particular scenario, which is determined by various factors such as field and in vitro multiplication rates of various genotypes, presence of bulbils in a part of the genepool, the sample size of the accessions as well as the number of stored accessions in cryopreservation. The comparative discussion is exemplified for a 1-year field rotation versus cryopreservation using either in vitro plantlets or a combination of bulbils and unripe inflorescence bases as organ sources. For the more expensive use of in vitro plants cryopreservation becomes less costly than field culture only after 13 years, whereas this is the case already after 8-9 years when using a combination of bulbils in winter and inflorescence bases in summer.

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“…Seeds are preferable to cuttings or plantlets from in vitro culture because they are cheap to produce and easy to obtain from large field genebanks established worldwide (Dulloo et al 2009). In addition, they represent the base propagation unit for the autogamous species C. arabica and can be efficiently used for identification of resistant accessions in allogamous species (Bertrand et al 2000b).…”

Section: Discussionmentioning

confidence: 99%

A high-throughput method for early screening of coffee (Coffea spp.) genotypes for resistance to root-knot nematodes (Meloidogyne spp.)

Villain

Aribi²,

Réversat³

et al. 2010

Eur J Plant Pathol

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Root-knot nematodes (Meloidogyne spp.) threaten the livelihood of millions of farmers producing coffee worldwide. The use of resistant plants either as cultivars or rootstocks appears to be the single most effective method of control. A screening method was developed to evaluate large populations of plants for resistance to root-knot nematodes. Two coffee cultivars, one susceptible and the other resistant to Meloidogyne paranaensis, were grown under controlled conditions in two substrates: a commercial sieved potting compost and an inert substrate containing sand with a water-absorbent synthetic polymer. Plant growth and development and nematode multiplication were compared for two inoculation dates (2 and 8 weeks after planting) and two evaluation dates (eight and 13 weeks after inoculation). Root growth, but not nematode multiplication, was influenced by the choice of substrate. Evaluation of the differences in root weight and nematode numbers between the different cultivars, substrates and dates of inoculation suggested that an optimal condition could be defined. The best discrimination between susceptible and resistant plants was found in the experiment where inoculation occurred at 2 weeks after planting and evaluation occurred at 8 weeks after inoculation. Because the total duration of this experiment was only 3 months, highthroughput evaluation was possible, opening up new possibilities for screening large germplasm collections and studying the genetic control of root-knot nematode resistance in coffee.

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Cost Efficiency of Cryopreservation as a Long‐Term Conservation Method for Coffee Genetic Resources

Cited by 43 publications

References 24 publications

Exploratory studies for cryopreservation of Coffea arabica L. seeds

Exploratory studies for cryopreservation of Coffea arabica L. seeds

Comparing costs for different conservation strategies of garlic (Allium sativum L.) germplasm in genebanks

A high-throughput method for early screening of coffee (Coffea spp.) genotypes for resistance to root-knot nematodes (Meloidogyne spp.)

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