Cortinarius section orellani: Isolation and culture of Cortinarius orellanus

Rapior, Sylvie; Andary, Claude; Mousain, Daniel

doi:10.1016/s0007-1536(87)80055-4

Cited by 13 publications

(6 citation statements)

References 6 publications

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“…Mycelia were grown in the dark, at 24°C, in standard medium (modified Pachlevski-Oddoux medium (22)) either in liquid cultures without shaking (23) or on agar-solidified medium (13). For Northern blot analyses, mycelia were grown in standard liquid medium containing ϳ5 mM K ϩ at pH 6.0 for 15 days and then transferred for 3 further days either in fresh standard medium (control treatment), in K ϩ -free medium (potassium salts being replaced by ammonium salts in the standard medium), in Na ϩ supplemented medium (standard medium supplemented with 3 mM NaCl), or in standard medium adjusted to pH 4.5 or 8.0 with HCl or KOH, respectively.…”

Section: Methodsmentioning

confidence: 99%

Molecular and Functional Characterization of a Na+-K+ Transporter from the Trk Family in the Ectomycorrhizal Fungus Hebeloma cylindrosporum

Corratgé

Zimmermann

Lambilliotte

et al. 2007

Journal of Biological Chemistry

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Ectomycorrhizal symbiosis between fungi and woody plants strongly improves plant mineral nutrition and constitutes a major biological process in natural ecosystems. Molecular identification and functional characterization of fungal transport systems involved in nutrient uptake are crucial steps toward understanding the improvement of plant nutrition and the symbiotic relationship itself. In the present report a transporter belonging to the Trk family is identified in the model ectomycorrhizal fungus Hebeloma cylindrosporum and named HcTrk1. The Trk family is still poorly characterized, although it plays crucial roles in K ؉ transport in yeasts and filamentous fungi. In Saccharomyces cerevisiae K ؉ uptake is mainly dependent on the activity of Trk transporters thought to mediate H ؉ :K ؉ symport. The ectomycorrhizal HcTrk1 transporter was functional when expressed in Xenopus oocytes, enabling the first electrophysiological characterization of a transporter from the Trk family. HcTrk1 mediates instantaneously activating inwardly rectifying currents, is permeable to both K ؉ and Na ؉ , and displays channel-like functional properties. The whole set of data and particularly a phenomenon reminiscent of the anomalous mole fraction effect suggest that the transport does not occur according to the classical alternating access model. Permeation appears to occur through a single-file pore, where interactions between Na ؉ and K ؉ might result in Na ؉ :K ؉ co-transport activity. HcTrk1 is expressed in external hyphae that explore the soil when the fungus grows in symbiotic condition. Thus, it could play a major role in both the K ؉ and Na ؉ nutrition of the fungus (and of the plant) in nutrient-poor soils.

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Section: Methodsmentioning

confidence: 99%

Molecular and Functional Characterization of a Na+-K+ Transporter from the Trk Family in the Ectomycorrhizal Fungus Hebeloma cylindrosporum

Corratgé

Zimmermann

Lambilliotte

et al. 2007

Journal of Biological Chemistry

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“…Thin layer chromatography (TLC) methods for the determination of orellanine have been developed [5][6][7]; however, most of these methods have been applied to mushroom tissues. Even electron spin resonance spectroscopy (ESR) analysis of the oxidation product from orellanine was described in [6] as a diagnostic tool.…”

Section: Introductionmentioning

confidence: 99%

A direct gas chromatography-mass spectrometry (GC-MS) method for the detection of orellanine present in stomach content (Part I)

Brondz¹,

Nevo²,

Wasser³

et al. 2012

JBPC

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Intoxication with Cortinarius orellanus Fr. is often lethal or, at the least, disabling for the victim. Orellanine is recognized as the prime toxic substance in this mushroom. Poisoning by other toxic mushrooms can be occasionally mistaken as poisoning by C. orellanus or vice versa. The C. orellanus toxins have a prolonged latent period after ingestion and onset in the appearance of symptoms. These properties of the toxin together with the chemo-, thermo- and photolability have made it difficult to develop a direct analytical method for diagnosing poisoning with orellanine, which in turn is needed to administer the correct medication. The aim of this study was to develop a direct analytical method for the detection of orellanine present in stomach content. Gas chromatography-mass spectrometry with supersonic molecular beams (GC-MS with SMB) was used for the direct detection of orellanine in the stomach fluids of rats after they were fed with food containing C. orellanus. This method can be used as a platform for the future development of analytical procedures for the direct analytical detection of orellanine in humans intoxicated by ingestion of toxic mushrooms. The standard orellanine was isolated from C. orellanus following the procedure described by Prast et al. [1] and was used as an authentic comparison

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“…var. cedretorum Bon obtenu sur milieu Melin-Norkrans modifié par Marx [20] (MNM), à partir d'un basidiome récolté dans la cédraie de Seheb (Moyen Atlas, Maroc) établie sur des limons argileux (pH 6,7) ; (ii) un isolat d'Hebeloma sinapizans (Paulet) Gillet obtenu sur le milieu PO utilisé par Rapior et al [32], à partir d'un basidiome récolté dans la cédraie de Belvezet (garrigue gardoise, France) sur rendzine plus au moins décarbo-natée, argileuse à argilo-limoneuse (pH 5,9). …”

Section: Matériel Végétal Et Fongiqueunclassified

Ectomycorhization de Cedrus atlantica en conditions contr�l�es : efficacit� de deux formes d'inoculum myc�lien

2002

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Résumé -L'effet de la forme de l'inoculum (obtenu sur tourbe-vermiculite ou par inclusion dans l'alginate) de Tricholoma tridentinum var. cedretorum et Hebeloma sinapizans sur la mycorhization de semis du Cèdre de l'Atlas, cultivés sur un substrat minéral, a été expérimenté en conditions contrôlées. L'utilisation de mycélium de T. tridentinum développé sur tourbe-vermiculite permet d'obtenir des degrés de mycorhization supérieurs à ceux enregistrés en employant du mycélium du même champignon inclus dans l'alginate, un an après l'inoculation. Le résultat inverse a été obtenu avec H. sinapizans. Les pourcentages d'apex mycorhizés et les teneurs en mycélium viable des racines des plants inoculés par T. tridentinum ont été supérieurs à ceux des plants inoculés avec H. sinapizans. L'obtention de mycorhizes à l'aide d'inocula mycéliens, a été réalisée sur le Cèdre de l'Atlas dans des conditions contrôlées et reproductibles. L'inoculation des semis par du mycélium d'H. sinapizans cultivé sur substrat solide a stimulé la biomasse de leurs parties aériennes et racinaires alors que l'inoculum de T. tridentinum, produit sur le même substrat, n'a permis d'augmenter significativement que la biomasse des racines. L'inoculation des plants par du mycélium d'H. sinapizans inclus dans l'alginate n'a été bénéfique que pour la biomasse de leurs parties aériennes. Cedrus atlantica / Tricholoma tridentinum var. cedretorum / mycorhization / inoculum mycélien / ergostérolAbstract -Ectomycorrhization of Cedrus atlantica seedlings under controlled conditions: Efficiency of two forms of mycelial inocula. The effect of two mycelial inocula (mycelia grown in peat-vermiculite saturated by a nutritive medium, or entrapped into alginate) of two ectomycorrhizal species (Tricholoma tridentinum var. cedretorum and Hebeloma sinapizans) was tested, under controlled conditions, on the mycorrhization of Cedar seedlings grown on mineral substrate. One year after inoculation, the percentage of infected root tips and root ergosterol contents were higher with cedars inoculated using T. tridentinum peat-vermiculite inoculum than using alginate one. The opposite results were obtained with H. sinapizans. The degrees of mycorrhization of the seedlings inoculated with T. tridentinum were higher than those of the seedlings inoculated with H. sinapizans. The mycorrhization of Cedrus atlantica seedlings using mycelial inocula was thus performed under controlled conditions which may be reproducible. The consequences of the inoculation of seedlings on their shoot and/or root biomass vary with the inoculum form and fungal species.Cedrus atlantica / Tricholoma tridentinum var. cedretorum / mycorrhization / mycelial inocula / ergosterol

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Cortinarius section orellani: Isolation and culture of Cortinarius orellanus

Cited by 13 publications

References 6 publications

Molecular and Functional Characterization of a Na+-K+ Transporter from the Trk Family in the Ectomycorrhizal Fungus Hebeloma cylindrosporum

Molecular and Functional Characterization of a Na+-K+ Transporter from the Trk Family in the Ectomycorrhizal Fungus Hebeloma cylindrosporum

A direct gas chromatography-mass spectrometry (GC-MS) method for the detection of orellanine present in stomach content (Part I)

Ectomycorhization de Cedrus atlantica en conditions contr�l�es : efficacit� de deux formes d'inoculum myc�lien

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