Corryocactus brevistylus (K. Schum. ex Vaupel) Britton & Rose (Cactaceae): Antioxidant, Gastroprotective Effects, and Metabolomic Profiling by Ultrahigh-Pressure Liquid Chromatography and Electrospray High Resolution Orbitrap Tandem Mass Spectrometry

Abstract

Corryocactus brevistylus (K. Schum. ex Vaupel) Britton & Rose (Cactaceae) is a shrubby or often arborescent cactus popularly known as "sancayo" and produce an edible fruit known as "Sanky" which is consumed in Arequipa-Perú. The purpose of this study was to report the gastroprotective activity and relate this activity to the antioxidant capacity and presence of phenolic compounds for the first time. A metabolomic profiling based on Ultrahigh-pressure liquid chromatography and electrospray high resolution mass spectrometry, and the antioxidant activities (DPPH, ABTS, and FRAP), ascorbic acid content, total phenolics and flavonoids contents, and the mode of gastroprotective action of the Sanky fruit including the involvement of prostaglandins, nitric oxide, and sulfhydryl compounds is reported. Thirty-eight compounds were detected in the ethanolic extract including 12 organic acids, nine hydroxycinnamic acids, three isoamericanol derivatives, six flavonoids, five fatty acids, and two sterols. The results of the biological tests showed that the ethanolic extract had antioxidant capacity and gastroprotective activity on the model of HCl/EtOH-induced gastric lesions in mice (at 10, 25, 50, and 100 mg/kg). The effect elicited by the extract at 50 mg/kg was reversed by indometacin and Nethylmaleimide but not by N G-nitro-L-arginine methyl ester suggesting that sulfhydryl groups and prostaglandins are involved in the mode of gastroprotective action. In

Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability

“…Both solutions were mixed in a 1:1 ratio ( v / v ) and incubated for 16 h in the dark for the formation of the radical ABTS •+ by the oxidation of ABTS with potassium persulfate at room temperature. Then, to 20 μL of the extract or standard to be measured (curve from 10–250 μg/mL), 200 μL of the previously ABTS •+ solution (adjusted with 80% methanol to an absorbance of 0.70 ± 0.02) was added, mixed using a vortex mixer and subsequently allowed to react in darkness at room temperature [ 35 ]. The absorbance was then measured at 765 nm after 6 min of reaction, and astaxanthin was used as a reference standard (curve from 10–250 μg/mL).…”

Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae)

“…Ultra-highperformance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HR-MS) is a modern and fast technique that allows the identification of accurate mass analysis. Moreover, is today a key tool for plant chemotaxonomy or metabolic profiling of plant samples (Simirgiotis et al, 2016b;Echiburu-Chau et al, 2017;Areche et al, 2020;Rodriguez et al, 2020).…”

“…Since herbal native infusions are receiving increasing attention for the number of physiological benefits they can bring to human health (Coelho et al, 2016) and as part of our continuing research of medicinal plants from Atacama Desert (Echiburu-Chau et al, 2017;Ardiles et al, 2018;Areche et al, 2020;Rodriguez et al, 2020) and native plants with potentiality as cholinesterase inhibitors (Barrientos et al, 2020a;Barrientos et al, 2020b), we investigated the infusions of the medicinal plant A. copa using screening tests to evaluate their antioxidant, antiprotozoal and enzyme inhibitory properties against cholinesterase and high-resolution UHPLC-MS/MS for the chemical profiling for the first time. Additionally, molecular docking of the major compounds was investigated.…”

Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry

“…Plant-based foods, especially fruits, have a great impact on human health [1,2]. Indeed, there is a growing body of scientific literature describing curative effects of fruits against a broad spectrum of diseases including diabetes, obesity, neurodegenerative, gastric and cardiovascular disorders, and certain types of cancers [3][4][5][6]. Different types of secondary metabolites have been reported in fruits [4,[7][8][9].…”

“…certain types of cancers [3][4][5][6]. Different types of secondary metabolites have been reported in fruits [4,[7][8][9].…”