Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry

Abstract

Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) is a Chilean endemic plant popularly known as “quiscal” and produces an edible fruit consumed by the local Mapuche communities named as “chupón”. In this study, several metabolites including phenolic acids, organic acids, sugar derivatives, catechins, proanthocyanidins, fatty acids, iridoids, coumarins, benzophenone, flavonoids, and terpenes were identified in G. sphacelata fruits using ultrahigh performance liquid chromatography-photodiode array detection coupled with a Orbitrap mass spectrometry (UHPLC-PDA-Orbitrap-MS) analysis for the first time. The fruits showed moderate antioxidant capacities (i.e., 487.11 ± 26.22 μmol TE/g dry weight) in the stable radical DPPH assay, 169.08 ± 9.81 TE/g dry weight in the ferric reducing power assay, 190.32 ± 6.23 TE/g dry weight in the ABTS assay, and 76.46 ± 3.18% inhibition in the superoxide anion scavenging assay. The cholinesterase inhibitory potential was evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). From the findings, promising results were observed for pulp and seeds. Our findings suggest that G. sphacelata fruits are a rich source of diverse secondary metabolites with antioxidant capacities. In addition, the inhibitory effects against AChE and BChE suggest that natural products or food supplements derived from G. sphacelata fruits are of interest for their neuroprotective potential.

UHPLC-MS Chemical Fingerprinting and Antioxidant, Antiproliferative, and Enzyme Inhibition Potential of Gaultheria pumila Berries

“…Ultra-high-resolution chromatography (UHPLC) coupled to mass spectrometry (MS) is a fast and modern technique that serves to provide information and compare the chemical profile of different metabolites that are naturally produced by plants [ 17 , 18 ]. Our group recently investigated the fingerprinting of Chilean native plants as well as their antioxidant and enzyme inhibitory properties [ 19 , 20 ]. In the present work, we report for the first time the chemical fingerprinting of G. pumila berries by UHPLC-MS analysis and the enzyme inhibition (against acetylcholinesterase, butyrylcholinesterase, and tyrosinase), as well as the antioxidant and antiproliferative activities.…”

Antihyperlipidemic and Antioxidant Capacities, Nutritional Analysis and UHPLC-PDA-MS Characterization of Cocona Fruits (Solanum sessiliflorum Dunal) from the Peruvian Amazon

“…Some oxylipins or unsaturated fatty acids were previously reported in edible fruits by some of us using TOF mass spectrometry [18]. In this study peak 37 [21], and peak 44 as syringaresinol 4-gentiobioside (C34H46O18), peak 53 as naringenin-7-Oglucoside (C21H22O10), [22] and peak 54 as methyl chlorogenate (C17H20O9) [23], finally, peak 59 was assigned as syringaresinol-glucoside (C28H36O13), and peak 65 as phloretin (C15H14O5). Several acyl quinic acids such as ferulic, sinapic and chlorogenic acid and derivatives showed different interesting bioactivities such as anti-inflammatory, vasorelaxant in mice aorta, platelet activation in human blood samples neuroprotective effects among others [24], while the glycoside derivative of the lignan syringaresinol enhanced ß-endorphin levels in rat plasma, [25] while syringaresinol aglycone was cytotoxic against human breast and lung cancer cells [26].…”

UHPLC-MS Metabolomic Fingerprinting, Antioxidant, and Enzyme Inhibition Activities of Himantormia lugubris from Antarctica

“…Ultra-high-resolution chromatography (UHPLC) coupled to mass spectrometry (MS) is a rapid and modern tool providing exact information and comparison of the chemical profile of different metabolites present in shrubs and fruits [18,19]. Our group has investigated the metabolome fingerprinting of Chilean native lichens and plants using this technique, and the antioxidant and enzyme inhibitory properties were also explored [20][21][22][23][24]. In this work, we report the metabolome fingerprinting of H. lugubris ethanolic extract by UHPLC-MS analysis, plus its antioxidant activity and enzyme inhibitory potential (against cholinesterase and tyrosinase), with full docking experiments of the extract and four isolated compounds for the first time.…”