Correlations between single nucleotide polymorphisms in FABP4 and meat quality and lipid metabolism gene expression in Yanbian yellow cattle

Abstract

FABP4 is a candidate gene for carcass and meat quality traits in livestock and poultry. However, the effects of FABP4 have not been examined in the Yanbian yellow cattle, an economically important local cattle breed in China. In this study, we characterized single nucleotide polymorphisms (SNPs) in FABP4 in this cattle breed and their associations with meat quality traits. Six SNPs (referred to as SNP1-6) were identified in FABP4 by direct sequencing and polymerase chain reaction-restriction fragment length polymorphism. The six SNPs were significantly correlated with meat quality traits. In particular, the GG and GA genotypes of SNP1 were significantly associated with water and fat contents and GG and AA genotypes of SNP1 were significantly associated with protein contents (P < 0.05). The fat content and marbling in heterozygous individuals at SNP2-6 were significantly higher than those in wild-type or mutant individuals (P < 0.05), while protein content was significantly higher in wild-type and mutant individuals than in heterozygous individuals (P < 0.05). A gene expression analysis indicated that the lipid metabolism-related genes FABP4, PPARγ, ANGPTL4, and LPL show similar expression patterns with respect to FABP4 genotypes, with the highest levels in wild-type individuals and the lowest levels in mutants. In conclusion, FABP4 SNPs can be used for marker-assisted selection in Yanbian yellow cattle breeding.

Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

“…We found that FABP4 was a downregulated DEM, which not only regulated the differentiation of bovine adipocytes in the PPAR signaling pathway and supported the transport of extracellular FAs but was also involved in lipolysis and FA transport in different tissues ( 71 , 72 ). A recent study on the FABP4 gene of Yanbian cattle showed that a SNP in FABP4 substantially affected the fat content, protein content, and marbling level ( 73 ). Li et al ( 74 ) found that with increasing age, the concentration of SFA in Yanbian cattle decreased, and the concentration of MUFA increased proportionally with an increase in the percentage of intramuscular lipids leading, in turn, to an increase in the expression of the FABP4 gene.…”

Lipogenic Gene Single Nucleotide Polymorphic DNA Markers Associated with Intramuscular Fat, Fat Melting Point, and Health-Beneficial Omega-3 Long-Chain Polyunsaturated Fatty Acids in Australian Pasture-Based Bowen Genetics Forest Pastoral Angus, Hereford, and Wagyu Beef Cattle

“…The fatty acid-binding protein-4 (FABP4) plays a role in the absorption, transport, and metabolism of long-chain fatty acids [ 66 , 67 , 68 ]. Previous studies reported an association between FABP4 polymorphism and carcass characteristics [ 35 ] and fat deposition [ 66 , 67 ]. In Japanese Black cattle, Hoashi et al [ 69 ], reported that genotype 174V of the FABP4 gene was significantly associated with 16:1 and 18:2.…”

“…SCD, FABP4, and FASN are lipogenic genes known to influence fat deposition and fatty acid metabolism in meat and adipose tissues [ 6 ]. SNP identification may further enhance our current understanding of the contribution of lipogenic genes to intramuscular fat deposition and fatty acid composition in the muscle tissue of diverse beef cattle breeds [ 35 , 36 , 37 , 38 , 39 ]. The identified SNP can be used effectively for marker-assisted selection to improve health-beneficial n-3 LC-PUFA and meat eating quality traits.…”

“…Identified SNP in FASN and FABP4 genes have been associated with marbling score and meat quality grade in Angus, Hereford, Holstein, Hanwoo, Yanbian Yellow, Qinchuan, and Brangus beef cattle [ 11 , 35 , 40 , 41 , 42 , 43 ]. Likewise, SCD SNP were reported to be associated with fat deposition and fatty acid composition in beef cattle breeds [ 44 , 45 , 46 , 47 ].…”

Towards Sustainable Sources of Omega-3 Long-Chain Polyunsaturated Fatty Acids in Northern Australian Tropical Crossbred Beef Steers through Single Nucleotide Polymorphisms in Lipogenic Genes for Meat Eating Quality

“…The FABP4 gene is an important protein for long-chain fatty acid transport in mammals, and its polymorphism is associated with growth, fat deposition, and carcass traits in cattle [66][67][68]. Substitution of the G to C allele of the g.44677587 (rs723716479) locus was positively correlated with CLA, previously inversely linked with the risk of colorectal and breast cancer in some population-based studies [69].…”