Correlations Between
            <i>LNCRNA-SOX2OT</i>
            Polymorphism and Susceptibility to Breast Cancer in a Chinese Population

Tang, Xiuwu; Gao, Yan; Yu, Lixin; Lü, Yan; Zhou, Guanglin; Liu, Cheng; Sun, Kai; Zhu, Baoli; Xu, Ming; Liu, Jingjing

doi:10.2217/bmm-2016-0238

Cited by 28 publications

(25 citation statements)

References 22 publications

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“…In another previous study, lncRNA Sox2ot expression was associated with T stage, distant metastasis, differentiation and a poorer OS and DFS time in breast cancer (17). Tang et al (19) reported that lncRNA Sox2ot was overexpressed in breast cancer tissues, and that a higher expression of lncRNA Sox2ot increased the risk of breast cancer for Chinese women. Shi et al (20) determined that the high expression of lncRNA Sox2ot was associated with the histological grade, the Tumor-Node-Metastasis stage, vein invasion and a relatively poor 5-year OS time in HCC, which was consistent with the results from the present study.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of lncRNA Sox2ot indicates a poor prognosis for patients with hepatocellular carcinoma and promotes cell invasion

Sun

Wei

2018

Oncol Lett

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Long non-coding RNA Sox2 overlapping transcript (lncRNA Sox2ot) expression has been demonstrated to be upregulated in a number of types of tumor, and may act as an oncogene. The present study aimed to evaluate the clinical role of lncRNA Sox2ot and its association with the epithelial-mesenchymal transition in hepatocellular carcinoma (HCC). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to determine the expression of lncRNA Sox2ot in 86 cases of HCC tissues and adjacent non-tumor tissues. Kaplan-Meier and log-rank test was used to analyze the association between lncRNA Sox2ot expression and disease-free (DFS) or overall (OS) survival time. In addition, the capacity of HCC cells with lncRNA Sox2ot knockdown for invasion was evaluated via transwell cell invasion assays. RT-qPCR and western blot analyses were also performed to determine the mRNA and protein expression of Twist1, E-cadherin and N-cadherin in the HCC cells. It was indicated that lncRNA Sox2ot expression levels were significantly higher in HCC tissues compared with adjacent non-tumor tissues. Increased expression levels of lncRNA Sox2ot were associated with the tumor size, the tumor number and vein invasion in patients with HCC. An association was observed between lncRNA Sox2ot and the DFS and OS of patients with HCC. Furthermore, it was determined that cell invasion was inhibited following the siRNA knockdown of lncRNA Sox2ot in MHCC97H and SMCC-7721 cells via transwell cell invasion assays. Furthermore, it was demonstrated that knockdown of lncRNA Sox2ot downregulated the mRNA and protein expression of Twist1 and N-cadherin, but upregulated the E-cadherin expression levels in MHCC97H and SMCC-7721 cells. Thus, it was indicated that lncRNA Sox2ot may be a novel predictive biomarker and a potential therapeutic target for HCC.

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Section: Discussionmentioning

confidence: 99%

Upregulation of lncRNA Sox2ot indicates a poor prognosis for patients with hepatocellular carcinoma and promotes cell invasion

Sun

Wei

2018

Oncol Lett

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“…Sox2 overlapping transcript (SOX2OT), a lncRNA transcribed in the same orientation as Sox2, has been shown to be upregulated and functions as oncogene in multiple types of cancers, including ovarian cancer,10 glioblastoma,11 breast cancer,12 gastric cancer,13 colorectal cancer,14 esophageal squamous cell carcinoma,15 and hepatocellular carcinoma 16. A study has shown that SOX2OT expression was upregulated in NSCLC tissues, closely associated with tumor-node-metastasis (TNM) stage, lymph node metastasis, and poor overall survival rate and that SOX2OT significantly promoted NSCLC cell proliferation by regulating cell cycle arrest at the G2/M phase 17.…”

Section: Introductionmentioning

confidence: 99%

Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132)

Zhang¹,

Yang²,

Qu³

et al. 2018

OTT

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BackgroundLong noncoding RNA (lncRNA) Sox2 overlapping transcript (SOX2OT) has been reported to be upregulated in various types of cancers, including non-small-cell lung cancer (NSCLC). However, the biological role and underlying mechanism of SOX2OT activity in NSCLC remain largely unknown. This study aims to investigate the function and possible molecular mechanisms of SOX2OT in NSCLC.Materials and methodsQuantitative real-time polymerase chain reaction was used to detect SOX2OT expression, and cellular proliferation, migration, and invasion were measured using cell counting kit-8, wound healing, and Transwell invasion assays, respectively. Western blotting was used to determine protein expression. Starbase 2.0 and luciferase reporter assay were utilized to identify the molecular target of SOX2OT.ResultsHere, we discovered that SOX2OT was markedly upregulated in NSCLC tissues and cell lines. Knockdown of SOX2OT inhibited the proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) process in NSCLC cells. Moreover, we explored the regulatory mechanism of SOX2OT and found that SOX2OT directly bound microRNA 132 (miR-132) in NSCLC cells. Importantly, miR-132 inhibition partially reversed the SOX2OT knockdown-mediated inhibitory effect on cell proliferation, migration, invasion, and EMT process. We also found that SOX2OT could regulate zinc finger E-box-binding homeobox 2 (a target of miR-132) expression, which played crucial roles in tumor cell proliferation and invasion.ConclusionThese findings indicated that SOX2OT was a noncoding oncogene that exerted important regulatory functions in NSCLC via sponging miR-132 and might represent a novel strategy for overcoming this disease.

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“…4A demonstrates that most of the results of RT-qPCR were consistent with microarray results. Out of these 5 lncRNAs, Pvt1 and SOX2OT have already been reported to exist in humans ( 20 , 21 ). Notably, SOX2OT is the most markedly downregulated gene, with a fold-change of −32.…”

Section: Resultsmentioning

confidence: 99%

“…RT-qPCR was introduced to validate microarray results and a total of 10 lncRNAs and mRNAs were included. In these lncRNAs, PVT1 and SOX2OT have already been reported and researched in humans, especially in cancer ( 20 , 21 ). PVT1 has also been studied intensively in DN ( 34 ).…”

Section: Discussionmentioning

confidence: 99%

Microarray analysis reveals long non‑coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy

et al. 2018

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Diabetic nephropathy (DN) is a highly complex syndrome involving multiple dysregulated biological processes. Long non-coding RNAs (lncRNAs) are now believed to have an important function in various diseases. However, their roles in DN remain largely unknown. Therefore, the present study was performed in order to investigate the lncRNAs that have a crucial role in DN. db/db mice were used as a DN model while db/m mice served as a control to search for lncRNAs which may have important roles in DN. Microarray and bioinformatics analysis gave an overview of the features of differentially expressed genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis demonstrated the typical biological alterations in DN. A co-expression network of lncRNAs and mRNAs revealed the complex interaction pattern in DN conditions. Further data investigation indicated that SOX2-overlapping transcript (SOX2OT), which was significantly downregulated in DN mice, may be the potentially functional lncRNA contributing to the onset of DN. The UCSC database demonstrated that SOX2OT was highly conserved in mice and humans. Additionally further study using cultured human podocytes and mesangial cells confirmed the downregulation of SOX2OT using reverse transcription-quantitative polymerase chain reaction and fluorescence in situ hybridization. However, the cellular location of SOX2OT depended on certain cell types. Taken together, the results of the present study indicated that SOX2OT may act as an important regulator in the pathogenesis of DN by interacting with various mRNAs with critical roles in DN.

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Correlations Between LNCRNA-SOX2OT Polymorphism and Susceptibility to Breast Cancer in a Chinese Population

Abstract: These results confirmed that BC was conspicuously associated with higher SOX2OT expression. SOX2OT SNP rs9839776 was significantly associated with the onset of BC possibly via influencing the expression of SOX2OT.

Cited by 28 publications

References 22 publications

Upregulation of lncRNA Sox2ot indicates a poor prognosis for patients with hepatocellular carcinoma and promotes cell invasion

Upregulation of lncRNA Sox2ot indicates a poor prognosis for patients with hepatocellular carcinoma and promotes cell invasion

Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132)

Microarray analysis reveals long non‑coding RNA SOX2OT as a novel candidate regulator in diabetic nephropathy

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