Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132)

Abstract

BackgroundLong noncoding RNA (lncRNA) Sox2 overlapping transcript (SOX2OT) has been reported to be upregulated in various types of cancers, including non-small-cell lung cancer (NSCLC). However, the biological role and underlying mechanism of SOX2OT activity in NSCLC remain largely unknown. This study aims to investigate the function and possible molecular mechanisms of SOX2OT in NSCLC.Materials and methodsQuantitative real-time polymerase chain reaction was used to detect SOX2OT expression, and cellular proliferation, migration, and invasion were measured using cell counting kit-8, wound healing, and Transwell invasion assays, respectively. Western blotting was used to determine protein expression. Starbase 2.0 and luciferase reporter assay were utilized to identify the molecular target of SOX2OT.ResultsHere, we discovered that SOX2OT was markedly upregulated in NSCLC tissues and cell lines. Knockdown of SOX2OT inhibited the proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) process in NSCLC cells. Moreover, we explored the regulatory mechanism of SOX2OT and found that SOX2OT directly bound microRNA 132 (miR-132) in NSCLC cells. Importantly, miR-132 inhibition partially reversed the SOX2OT knockdown-mediated inhibitory effect on cell proliferation, migration, invasion, and EMT process. We also found that SOX2OT could regulate zinc finger E-box-binding homeobox 2 (a target of miR-132) expression, which played crucial roles in tumor cell proliferation and invasion.ConclusionThese findings indicated that SOX2OT was a noncoding oncogene that exerted important regulatory functions in NSCLC via sponging miR-132 and might represent a novel strategy for overcoming this disease.

Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition

“…Accordingly, LncRNA Sox2ot can upregulate the expression of zinc finger E-box-binding homeobox 2 (a target of miR-132) via sponging miR-132 and results in triggering the non-small-cell lung cancer tumor cell proliferation and invasion [ 28 ]. Intriguingly, miR-194-5p has been elucidated as a target of lncRNA Sox2ot, however, AKT2 is known to target miR-194-5p.…”

The lncRNA SOX2OT rs9839776 C>T Polymorphism Indicates Recurrent Miscarriage Susceptibility in a Southern Chinese Population

“…The rs9839776 polymorphism in the SOX2OT gene is related to SOX2OT expression in breast cancer [22]. Furthermore, SOX2OT may regulate cell migration and invasion of multiple tumor cells [25, 27–29]. Moreover, trophoblast migration function plays an important role in the process of recurrent miscarriage.…”

“…Recent studies have found that lncRNA SOX2OT acts as a carcinogenic molecule in the pathogenesis of many human cancers, such as gastric cancer, colorectal cancer, and breast cancer [23–26]. Research has found that SOX2OT may regulate the migration and invasion of multiple tumor cells, including non-small-cell lung cancer cells, hepatocellular carcinoma cells, gastric cancer cells, and colorectal cancer cells [25, 27–29]. Recently, a study by Tang et al found that SOX2OT polymorphisms (rs9839776 C>T) are associated with breast cancer susceptibility via their influence on the expression of SOX2OT [22].…”

Molecular mechanisms of the microRNA-132 during tumor progressions

“…There were significant SOX2OT up regulations in Non-small-cell lung carcinoma (NSCLC) tissues and cell lines. SOX2OT silencing significantly reduced cell proliferation, invasion, and EMT process by miR-132 sponging that resulted in ZEB2 up regulation [ 80 ]. There was also a significant miR-132 down-regulation in metastatic CRC tissues in comparison with non-metastatic tumor tissues.…”