Correlation of in vitro platelet quality measurements with in vivo platelet viability in human subjects

Abstract

Background and Objectives Changes in in vitro platelet quality parameters during platelet storage are associated with a decrease of in vivo platelet viability after platelet transfusion. Many attempts have been made to identify the most predictable in vitro parameters for in vivo performance. We used a riboflavin-based ultraviolet (UV) light treatment process designed to inactivate pathogens and white blood cell (WBC) contaminants in blood products as a model system in which to study the correlation of in vitro cell quality with in vivo viability. Materials and MethodsPlatelet products ( n = 18) were collected by a standard Trima apheresis procedure and treated with one of three dose levels of UV light (0, 7·2 or 12·4 J/ml) in the presence of 50 µ M riboflavin. Lactate production, glucose consumption and P-selectin expression, pH, pCO 2 , pO 2 , hypotonic shock response and swirl were measured during 5 days of platelet storage post-UV/RB treatment. Aliquots of these products were radiolabelled on day 5 of storage and were subsequently used to determine platelet recovery and survival time in autologous subjects. ResultsThe responses of in vitro cell quality were observed to occur in a UV dosedependent manner. Lactate production and pH were identified as the parameters most strongly correlated with platelet in vivo recovery, which ranged from 5 to 82%. The correlation coefficients ( r ) for lactate production and pH with in vivo recovery in human subjects were 0·9090 and 0·8831 with P -values of 0·007 and 0·031, respectively. Lactate production and pH were also found to be correlated with platelet survival time, with correlation coefficients of 0·8063 and 0·8384 (the P values were 0·01 and 0·001, respectively).Conclusions Using conditions of riboflavin-based UV light treatment, lactate production and pH were identified as having the highest correlations with recovery and survival of radiolabelled platelets in healthy subjects.Key words: in vitro platelet quality, in vivo platelet recovery, platelet survival, platelet viability prediction, UV light treatment.

Treatment of Buffy Coat Platelets in Platelet Additive Solution with the Mirasol^® Pathogen Reduction Technology System

“…1 and 2), which were, however, well within the limits previously established for acceptable in vivo recovery and survival [11]. LDH measurements yielded comparable results for control and test units through day 5, with a slight increase in Mirasol-treated units observed on day 7.…”

Quality control of riboflavin-treated platelet concentrates using Mirasol® PRT system: Polish experience

“…21 In the 1970s, Valeri demonstrated a correlation between HSR and platelet viability in the recipient's circulatory system. 22 A similar correlation was reported by Handin, who recognized HSR as the most significant in vitro parameter to reflect in vivo platelet functions.…”

“…After 5 days of storage, the PCs in group M showed no statistically significant HSR differences compared to control PCs, which only shows that platelets are capable of "removing water out of the cell." 21,24 The turbidimetric method was used to evaluate platelet aggregation capacity by inducing with ADP, an agonist which defines the adhesion and aggregation capacity. ADPrelated aggregation depends on the presence of ADP receptors, IIb/IIIa receptors for fibrinogen and proper alpha granule release.…”

Pathogen-Inaktivierungssysteme für Thrombozytenkonzentrate

“…Glukoseverbrauch eine Korrelation zur Wiederfindung und Überlebensra-te der Thrombozyten in Probanden [118,119].…”