Correlation of endogenous β-glucuronidase activity with differentiation of in vitro cultures of Scutellaria baicalensis

Solov'eva, A. I.; Evsyukov, S. V.; Sidorov, Roman A.; Stepanova, A. Yu.

doi:10.1007/s11738-020-03159-0

Cited by 7 publications

(4 citation statements)

References 42 publications

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“…As it was shown previously, the S. baicalensis hairy roots cultivated on agar and in liquid nutrient media differed significantly from each other in the growth and content of the main flavones [ 38 ]. Therefore, in this study, we also compared these indicators in hairy roots growing on media of different density.…”

Section: Resultsmentioning

confidence: 93%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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We measured and studied the growth parameters and the qualitative and quantitative composition of the flavones of hairy roots of the Scutellaria genus: S. lateriflora, S. przewalskii and S. pycnoclada. Hairy roots were obtained using wild-type Agrobacterium rhizogenes A4 by co-cultivation of explants (cotyledons) in a suspension of Agrobacterium. The presence of the rol-genes was confirmed by PCR analysis. The hairy roots of the most studied plant from the Scutellaria genus, S. baicalensis, were obtained earlier and used as a reference sample. HPLC-MS showed the predominance of four main flavones (baicalin, baicalein, wogonin and wogonoside) in the methanol extracts of the studied hairy roots. In addition to the four main flavones, the other substances which are typical to the aerial part of plants were found in all the extracts: apigenin, apigetrin, scutellarin and chrysin-7-O-β-d-glucuronide. According to the total content of flavones, the hairy roots of the studied skullcaps form the following series: S. przewalskii (33 mg/g dry weight) > S. baicalensis (17.04 mg/g dry weight) > S. pycnoclada (12.9 mg/g dry weight) > S. lateriflora (4.57 mg/g dry weight). Therefore, the most promising producer of anti-coronavirus flavones is S. przewalskii.

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Section: Resultsmentioning

confidence: 93%

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

et al. 2021

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“…It should be noted that undifferentiated cultures (calli and suspensions) of S. baicalensis had a different ratio of flavones with a predominance of the baicalin/baicalein pair, in contrast to hairy roots. Interestingly, sGUS activity in hairy roots was 10 times higher than in undifferentiated cultures [137]. In the future, we plan to study the possibilities of sGUS activation and an increase in the level of biologically active flavones-aglycones.…”

Section: The Collection Of Hairy Roots Of the Institute Of Plant Phys...mentioning

confidence: 94%

“…The genetic stability of hairy roots and their ability to maintain the synthesis of low molecular weight metabolites under in vitro conditions at the level of the roots of the whole plant formed the basis for studies conducted with the roots of valuable medicinal plants of the genus Scutellaria. The roots of Baikal skullcap, which have been steadily growing for more than 25 years, synthesize flavones typical of the roots of this plant-glucuronides: baicalin and wogonoside, and aglycones: baicalein and wogonin [134][135][136][137][138] (Figure 7). The genetic stability of hairy roots and their ability to maintain the synthesis of low molecular weight metabolites under in vitro conditions at the level of the roots of the whole plant formed the basis for studies conducted with the roots of valuable medicinal plants of the genus Scutellaria.…”

Section: The Collection Of Hairy Roots Of the Institute Of Plant Phys...mentioning

confidence: 99%

Collection of Hairy Roots as a Basis for Fundamental and Applied Research

et al. 2022

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Due to population growth, instability of climatic conditions, and reduction of the areas of natural ecosystems, it becomes necessary to involve modern biotechnological approaches to obtain highly productive plant material. This statement applies both to the creation of plant varieties and the production of new pharmaceutical raw materials. Genetic transformation of valuable medicinal plants using Agrobacterium rhizogenes ensures the production of stable and rapidly growing hairy roots cultures that have a number of advantages compared with cell culture and, above all, can synthesize root-specific substances at the level of the roots of the intact plant. In this regard, special attention should be paid to the collection of hairy roots of the Institute of Plant Physiology RAS, Russian Academy of Sciences, the founder of which was Dr. Kuzovkina I.N. Currently, the collection contains 38 hairy roots lines of valuable medicinal and forage plants. The review discusses the prospects of creating a hairy roots collection as a basis for fundamental research and commercial purposes.

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“…Some improvement in yields of flavones from the cultures was achieved as well by an optimization of the nutrient medium composition [ 96 , 105 , 106 ]. Aglycones:glucuronides ratios in the cultures may be at least partially controlled by the activities of the endogenous β-glucuronidase of Baikal skullcap (baicalinase, sGUS) and baicalein 7- O -glucuronosyltransferase (UBGAT) [ 107 , 108 ]. Hydroxylation of chrysin is the step in the biosynthesis of baicalein and wogonin that requires the respective CYP450 enzymes: flavone 6-hydroxylase (F6H) and flavone 8-hydroxylase (F8H).…”

Section: Polyphenolic Antioxidants In Hrsmentioning

confidence: 99%

Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Malarz

Michalska

Yudina

et al. 2022

Plants

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Due to their chemical properties and biological activity, antioxidants of plant origin have gained interest as valuable components of the human diet, potential food preservatives and additives, ingredients of cosmetics and factors implicated in tolerance mechanisms against environmental stress. Plant polyphenols are the most prominent and extensively studied, albeit not only group of, secondary plant (specialized) metabolites manifesting antioxidative activity. Because of their potential economic importance, the productive and renewable sources of the compounds are desirable. Over thirty years of research on hairy root cultures, as both producers of secondary plant metabolites and experimental systems to investigate plant biosynthetic pathways, brought about several spectacular achievements. The present review focuses on the Rhizobium rhizogenes-transformed roots that either may be efficient sources of plant-derived antioxidants or were used to elucidate some regulatory mechanisms responsible for the enhanced accumulation of antioxidants in plant tissues.

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Correlation of endogenous β-glucuronidase activity with differentiation of in vitro cultures of Scutellaria baicalensis

Cited by 7 publications

References 42 publications

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Hairy Roots of Scutellaria spp. (Lamiaceae) as Promising Producers of Antiviral Flavones

Collection of Hairy Roots as a Basis for Fundamental and Applied Research

Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

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