Collection of Hairy Roots as a Basis for Fundamental and Applied Research

Abstract

Due to population growth, instability of climatic conditions, and reduction of the areas of natural ecosystems, it becomes necessary to involve modern biotechnological approaches to obtain highly productive plant material. This statement applies both to the creation of plant varieties and the production of new pharmaceutical raw materials. Genetic transformation of valuable medicinal plants using Agrobacterium rhizogenes ensures the production of stable and rapidly growing hairy roots cultures that have a number of advantages compared with cell culture and, above all, can synthesize root-specific substances at the level of the roots of the intact plant. In this regard, special attention should be paid to the collection of hairy roots of the Institute of Plant Physiology RAS, Russian Academy of Sciences, the founder of which was Dr. Kuzovkina I.N. Currently, the collection contains 38 hairy roots lines of valuable medicinal and forage plants. The review discusses the prospects of creating a hairy roots collection as a basis for fundamental research and commercial purposes.

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

“…The authors declare no conflict of interest. * The collection of hairy root culture was comprehensively covered earlier [17].…”

“…A collection of HR cultures was organized at IPPRAS in the 1980s [ 94 ] and currently includes 38 HR lines belonging to 25 plant species and nine families. The history, composition, and use of the HR collection have been recently reviewed [ 17 ].…”

“…Passport development for new cell lines 3. Cell culture deposit (maintenance) for the patent purpose Currently not available Long-term conservation of botanical, agricultural, and biotechnological collections * The collection of hairy root culture was comprehensively covered earlier [ 17 ]. …”

“…Unlike crop genebanks and botanic gardens, little is known about genetic collections of biotechnologically valuable plant materials in vitro, e.g., plant cell and hairy root cultures, embryogenic cell lines, in vitro clones with superior qualities, etc. Information about some of these collections can be driven from publications [ 15 , 16 , 17 ] or open online resources, while others belong to commercial companies and are protected by patents and commercial confidentiality laws.…”